| Entry |
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| Name |
glucosaminate ammonia-lyase;
glucosaminic dehydrase;
D-glucosaminate dehydratase;
D-glucosaminic acid dehydrase;
aminodeoxygluconate dehydratase;
2-amino-2-deoxy-D-gluconate hydro-lyase (deaminating);
aminodeoxygluconate ammonia-lyase;
2-amino-2-deoxy-D-gluconate ammonia-lyase;
D-glucosaminate ammonia-lyase;
D-glucosaminate ammonia-lyase (isomerizing;
2-dehydro-3-deoxy-D-gluconate-forming)
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| Class |
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
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| Sysname |
2-amino-2-deoxy-D-gluconate ammonia-lyase (isomerizing; 2-dehydro-3-deoxy-D-gluconate-forming)
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| Reaction(IUBMB) |
2-amino-2-deoxy-D-gluconate = 2-dehydro-3-deoxy-D-gluconate + NH3 (overall reaction) [RN: R01544];
(1a) 2-amino-2-deoxy-D-gluconate = (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate + H2O [RN: R10773];
(1b) (2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate = (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate (spontaneous) [RN: R10774];
(1c) (4S,5R)-4,5,6-trihydroxy-2-iminohexanoate + H2O = 2-dehydro-3-deoxy-D-gluconate + NH3 (spontaneous) [RN: R10775]
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| Reaction(KEGG) |
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| Substrate |
2-amino-2-deoxy-D-gluconate [CPD: C03752];
(2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate [CPD: C20844];
(4S,5R)-4,5,6-trihydroxy-2-iminohexanoate [CPD: C20845];
H2O [CPD: C00001]
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| Product |
2-dehydro-3-deoxy-D-gluconate [CPD: C00204];
NH3 [CPD: C00014];
(2Z,4S,5R)-2-amino-4,5,6-trihydroxyhex-2-enoate [CPD: C20844];
H2O [CPD: C00001];
(4S,5R)-4,5,6-trihydroxy-2-iminohexanoate [CPD: C20845]
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| Comment |
Contains pyridoxal phosphate. The enzyme releases an unstable enamine product that tautomerizes to an imine form, which undergoes spontaneous hydrolytic deamination to form the final product.
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| History |
EC 4.3.1.9 created 1972, (EC 4.3.1.21 created 1965 as EC 4.2.1.26, transferred 2002 to EC 4.3.1.21, incorporated 2004) modified 2004
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| Pathway |
| Pentose phosphate pathway | | Metabolic pathways |
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| Reference |
1 |
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| Authors |
Imanaga, Y. |
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| Title |
Metabolism of D-glucosamine. III. Enzymic degradation of D-glucosaminic acid. |
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| Journal |
J. Biochem. (Tokyo) 45 (1958) 647-650. |
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| Reference |
2 |
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| Authors |
Merrick, J.M. and Roseman, S. |
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| Title |
D-Glucosaminic acid dehydrase. |
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| Journal |
Methods Enzymol. 9 (1966) 657-660. |
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| Reference |
|
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| Authors |
Iwamoto R, Imanaga Y, Soda K. |
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| Title |
D-glucosaminate dehydratase from Agrobacterium radiobacter. Physicochemical and enzymological properties. |
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| Journal |
J. Biochem. (Tokyo). 91 (1982) 283-9. |
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| Reference |
|
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| Authors |
Iwamoto R, Taniki H, Koishi J, Nakura S. |
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| Title |
D-glucosaminate aldolase activity of D-glucosaminate dehydratase from Pseudomonas fluorescens and its requirement for Mn2+ ion. |
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| Journal |
Biosci. Biotechnol. Biochem. 59 (1995) 408-11. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 37290-91-8 |
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