KEGG   ENZYME: 1.14.14.3Help
Entry
EC 1.14.14.3                Enzyme                                 
Name
alkanal monooxygenase (FMN);
bacterial luciferase;
aldehyde monooxygenase;
luciferase;
Vibrio fischeri luciferase;
alkanal,reduced-FMN:oxygen oxidoreductase (1-hydroxylating, luminescing);
alkanal,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing)
Class
Oxidoreductases;
Acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
aldehyde,FMNH2:oxygen oxidoreductase (1-hydroxylating, luminescing)
Reaction(IUBMB)
an aldehyde + FMNH2 + O2 = a carboxylate + FMN + H2O + hnu [RN:R03536]
Reaction(KEGG)
Substrate
aldehyde [CPD:C00071];
FMNH2 [CPD:C01847];
O2 [CPD:C00007]
Product
carboxylate [CPD:C00060];
FMN [CPD:C00061];
H2O [CPD:C00001];
hnu [CPD:C00205]
Comment
The reaction sequence involves incorporation of a molecule of oxygen into reduced FMN, and subsequent reaction with the aldehyde to form an activated FMN.H2O complex, which breaks down with emission of light. The enzyme is highly specific for reduced FMN, and for long-chain aliphatic aldehydes with eight carbons or more.
Orthology
K00494  
alkanal monooxygenase alpha chain
K15854  
alkanal monooxygenase beta chain
Genes
PLU: 
plu2081(luxA) plu2082(luxB)
PAY: 
PAU_02511(luxB) PAU_02512(luxA)
VHA: 
VFI: 
VF_A0920(luxB) VF_A0921(luxA)
VFM: 
VSA: 
SWD: 
Taxonomy
Reference
1  [PMID:363350]
  Authors
Hastings JW.
  Title
Bacterial bioluminescence light emission in the mixed function oxidation of reduced flavin and fatty aldehyde.
  Journal
CRC. Crit. Rev. Biochem. 5 (1978) 163-84.
Reference
2  [PMID:199107]
  Authors
Hastings JW, Nealson KH.
  Title
Bacterial bioluminescence.
  Journal
Annu. Rev. Microbiol. 31 (1977) 549-95.
Reference
3  [PMID:309549]
  Authors
Hastings JW, Presswood RP.
  Title
Bacterial luciferase: FMNH2-aldehyde oxidase.
  Journal
Methods. Enzymol. 53 (1978) 558-70.
Reference
4  [PMID:396467]
  Authors
Nealson KH, Hastings JW.
  Title
Bacterial bioluminescence: its control and ecological significance.
  Journal
Microbiol. Rev. 43 (1979) 496-518.
  Organism
Vibrio harveyi [GN:vha], Vibrio cholerae, Beneckea splendida, Photobacterium fischeri, Photobacterium phosphoreum, Photobacterium leiognathi, Vibrio albensis, Xenorhabdus luminescencens
Reference
5
  Authors
Suzuki, K., Kaidoh, T., Katagiri, M. and Tsuchiya, T.
  Title
O2 incorporation into a long-chain fatty-acid during bacterial luminescence.
  Journal
Biochim. Biophys. Acta 722 (1983) 297-301.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9014-00-0
DBGET integrated database retrieval system