KEGG   ENZYME: 3.2.2.5Help
Entry
EC 3.2.2.5                  Enzyme                                 

Name
NAD+ glycohydrolase;
NAD glycohydrolase;
nicotinamide adenine dinucleotide glycohydrolase;
beta-NAD+ glycohydrolase;
DPNase (ambiguous);
NAD hydrolase (ambiguous);
diphosphopyridine nucleosidase (ambiguous);
nicotinamide adenine dinucleotide nucleosidase (ambiguous);
NAD nucleosidase (ambiguous);
DPN hydrolase (ambiguous);
NADase (ambiguous);
nga (gene name);
NAD+ nucleosidase
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
NAD+ glycohydrolase
Reaction(IUBMB)
NAD+ + H2O = ADP-D-ribose + nicotinamide [RN:R00102]
Reaction(KEGG)
Substrate
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Product
ADP-D-ribose [CPD:C00301];
nicotinamide [CPD:C00153]
Comment
This enzyme catalyses the hydrolysis of NAD+, without associated ADP-ribosyl cyclase activity (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. The enzyme from the venom of the snake Agkistrodon acutus also catalyses EC 3.6.1.5, apyrase [3].
History
EC 3.2.2.5 created 1961, modified 2013
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
Orthology
K18153  NAD+ glycohydrolase
K19980  ADP-ribosyltransferase 2 / NAD+ glycohydrolase
Genes
MMU: 11871(Art2a-ps) 11872(Art2b)
RNO: 293152(Art2b)
CGE: 100766862 100767157 100771938 100772803
NGI: 103749849
HGL: 106007650
CCAN: 109678990
TUP: 102487179
BACU: 103000152
MYB: 102252898
MYD: 102773420
PALE: 102895427
SPY: SPy_0165(nga)
SPZ: M5005_Spy0139(nga)
SPYM: M1GAS476_0178(nga)
SPM: spyM18_0163(nga)
SPG: SpyM3_0128(nga)
SPS: SPs0130
SPJ: MGAS2096_Spy0146(nga)
SPK: MGAS9429_Spy0141(nga)
SPF: SpyM50134(nga)
SPB: M28_Spy0137(nga)
STG: MGAS15252_0175(nga)
SOZ: Spy49_0144(nga)
STX: MGAS1882_0175(nga)
SPYA: A20_0190(nga)
SPYH: L897_00945
SEU: SEQ_0311
SDS: SDEG_2029(nga)
SDA: GGS_1869(nga)
SDC: SDSE_2125(nga)
SDQ: SDSE167_2131(nga)
 » show all
Taxonomy
Reference
1  [PMID:4322210]
  Authors
Fehrenbach FJ
  Title
[Purification and crystallization of NAD glycohydrolase of C streptococci].
  Journal
Eur. J. Biochem. 18 (1971) 94-102.
Reference
2  [PMID:236282]
  Authors
Grushoff PS, Shany S, Bernheimer AW
  Title
Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase.
  Journal
J. Bacteriol. 122 (1975) 599-605.
Reference
3  [PMID:18952139]
  Authors
Zhang L, Xu X, Luo Z, Shen D, Wu H
  Title
Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus.
  Journal
Biochimie. 91 (2009) 240-51.
Reference
4  [PMID:20018886]
  Authors
Ghosh J, Anderson PJ, Chandrasekaran S, Caparon MG
  Title
Characterization of Streptococcus pyogenes beta-NAD+ glycohydrolase: re-evaluation of enzymatic properties associated with pathogenesis.
  Journal
J. Biol. Chem. 285 (2010) 5683-94.
Reference
5  [PMID:21300288]
  Authors
Smith CL, Ghosh J, Elam JS, Pinkner JS, Hultgren SJ, Caparon MG, Ellenberger T
  Title
Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin.
  Journal
Structure. 19 (2011) 192-202.
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.5
IUBMB Enzyme Nomenclature: 3.2.2.5
ExPASy - ENZYME nomenclature database: 3.2.2.5
BRENDA, the Enzyme Database: 3.2.2.5
CAS: 9025-46-1

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