KEGG   ENZYME: 3.4.21.107Help
Entry
EC 3.4.21.107               Enzyme                                 

Name
peptidase Do;
DegP;
DegP protease;
HtrA;
high temperature requirement protease A;
HrtA heat shock protein;
protease Do;
Do protease
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val!Val
Comment
This serine endopeptidase is essential for the clearance of denatured or aggregated proteins from the inner-membrane and periplasmic space in Escherichia coli. Natural substrates of the enzyme include colicin A lysis protein, pilin subunits and MalS from E. coli [3]. The enzyme has weak peptidase activity with casein and other non-native substrates [3]. The peptidase acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures [1,6]. Molecular chaperones and peptidases control the folded state of proteins by recognizing hydrophobic stretches of polypeptide that become exposed by misfolding or unfolding. They then bind these hydrophobic substrates to prevent aggregation or assist in protein refolding. If attempts at refolding fail, then irreversibly damaged proteins are degraded by peptidases such as this enzyme [6]. Belongs in peptidase family S1C.
History
EC 3.4.21.107 created 2006
Orthology
K04771  
serine protease Do
Genes
ECO: 
b0161(degP)
ECJ: 
Y75_p0158(degP)
ECD: 
EBW: 
BWG_0154(degP)
ECOK: 
ECE: 
Z0173(htrA)
ECS: 
ECF: 
ETW: 
ECSP_0162(degP)
ELX: 
EOJ: 
EOI: 
EOH: 
ECG: 
EOK: 
ELR: 
ECC: 
c0197(htrA)
ECP: 
ECI: 
ECV: 
ECX: 
ECW: 
ECM: 
ECY: 
ECR: 
ECQ: 
ECK: 
ECT: 
EOC: 
CE10_0165(degP)
EUM: 
ECZ: 
ELO: 
ELN: 
ELH: 
ESE: 
ESO: 
ESM: 
ESL: 
ECL: 
EBR: 
ECB_00160(degP)
EBD: 
EKO: 
EKF: 
EAB: 
EDH: 
EDJ: 
EIH: 
ENA: 
ELU: 
EUN: 
ELW: 
ECW_m0158(degP)
ELL: 
WFL_00785(degP)
ELC: 
i14_0182(htrA)
ELD: 
i02_0182(htrA)
ELP: 
EBL: 
ECD_00160(degP)
EBE: 
B21_00159(degP)
ELF: 
LF82_0461(degP)
ECOA: 
ECOL: 
ECOI: 
ECOJ: 
EFE: 
EFER_0184(degP)
STY: 
STY0231(htrA)
STT: 
t0210(htrA)
SEX: 
SENT: 
STM: 
STM0209(htrA)
SEO: 
SEV: 
SEY: 
SEM: 
SEJ: 
SEB: 
SEF: 
SETU: 
SETC: 
SEEN: 
SENR: 
SEND: 
SPT: 
SPA0215(htrA)
SEK: 
SPQ: 
SEI: 
SPC_0225(htrA)
SEC: 
SC0209(htrA)
SEH: 
SHB: 
SENH: 
SEEH: 
SEE: 
SENN: 
SEW: 
SEA: 
SENS: 
SED: 
SeD_A0229(degP)
SEG: 
SG0213(htrA)
SEL: 
SPUL_0228(htrA)
SEGA: 
SET: 
SEN0214(htrA)
SENJ: 
SEEC: 
SEEB: 
SEEP: 
SENB: 
BN855_2250(SBOV01761)
SENE: 
SES: 
SBG: 
SBG_0199(htrA)
SBZ: 
YPE: 
YPO3382(gsrA)
YPK: 
y0807(htrA)
YPA: 
YPN: 
YPM: 
YP_0303(gsrA)
YPP: 
YPG: 
YPZ: 
YPZ3_2979(gsrA)
YPT: 
YPD: 
YPX: 
YPD8_2963(gsrA)
YPH: 
YPC_3708(htrA)
YPS: 
YPTB0749(htrA)
YPI: 
YPY: 
YPB: 
YEN: 
YE0741(degP)
YEP: 
YEY: 
SFL: 
SF0153(htrA)
SFX: 
S0156(htrA)
SFV: 
SFV_0146(htrA)
SFE: 
SFxv_0161(degP)
SSN: 
SSON_0174(htrA)
SSJ: 
SBO: 
SBO_0150(htrA)
SBC: 
SDY: 
SDY_0178(htrA)
SDZ: 
ECA: 
ECA3301(degP)
PCT: 
PCC: 
PWA: 
PEC: 
ETA: 
EPY: 
EPR: 
EAM: 
EAMY_2765(htrA)
EAY: 
EAM_0814(degP)
EBI: 
EbC_08040(gsrA)
ERJ: 
BUC: 
BU228(htrA)
BAP: 
BAU: 
BAW: 
BAJC: 
BUA: 
BUP: 
BAK: 
BAKON_229(degP)
BUH: 
BUAMB_210(degP)
BAS: 
BUsg222(htrA)
BAB: 
bbp210(htrA)
SGL: 
ENT: 
ENC: 
ENO: 
ECLO: 
EEC: 
ENL: 
ESC: 
EAS: 
EAE: 
EAR: 
ESA: 
CSK: 
ES15_3167(degP)
CSZ: 
CSI: 
CTU: 
CTU_07920(degP)
KPN: 
KPN_00176(degP)
KPU: 
KP1_1014(degP)
KPM: 
KPP: 
KPE: 
KPK_4557(degP)
KPO: 
KPR: 
KPR_1107(degP)
KPJ: 
KPI: 
KVA: 
KOX: 
KOE: 
CKO: 
CRO: 
ROD_01651(htrA)
SPE: 
SRR: 
SRL: 
SRS: 
SRA: 
SSZ: 
SCc_148(htrA)
SMAF: 
SMW: 
SLQ: 
EIC: 
ETR: 
ETD: 
ETC: 
HDE: 
HDEF_1479(degP_1) HDEF_1488(degP_2)
DDA: 
DDC: 
DDD: 
DZE: 
PAM: 
PANA_0785(degP)
PLF: 
PAJ: 
PAJ_0131(degP)
PAQ: 
PVA: 
Pvag_0196(htrA)
PAO: 
RAH: 
RAQ: 
RAA: 
EBT: 
ROR: 
EBF: 
HDU: 
HD0260(degP)
HSO: 
HS_0816(htrA)
PMU: 
PM0734(htrA)
APL: 
APL_1293(htrA)
APJ: 
APJL_1306(degP)
XFA: 
XFT: 
PD0231(htrA)
XOO: 
XOO0059(htrA)
XOM: 
VCH: 
VCE: 
VCO: 
VCR: 
VCM: 
VCI: 
VVU: 
VVY: 
VPA: 
VHA: 
VEX: 
VFU: 
VFI: 
VF_2225(degP)
CPS: 
CPS_4346(degP)
CBU: 
CBU_0755(degP.2)
CBS: 
CBD: 
CBUD_0769(degP.1)
CBG: 
CbuG_1247(htrA)
CBC: 
CbuK_0614(htrA)
LPN: 
lpg1331(htrA)
LPF: 
lpl1284(htrA)
LPP: 
lpp1285(htrA)
LPC: 
LPC_0747(htrA)
LPA: 
LLO: 
LLO_2348(htrA)
MCA: 
MCA2343(htrA)
NMU: 
HPY: 
HPJ: 
jhp0405(htrA)
HPA: 
HPS: 
HPG: 
HPG27_410(htrA)
HPP: 
HPB: 
HPL: 
HPB8_1134(htrA)
HPC: 
HPM: 
HHE: 
HH0454(htrA)
HAC: 
Hac_1123(htrA)
HMS: 
HMU01920(htrA)
HBI: 
WSU: 
WS2226(HTRA)
TDN: 
CJE: 
Cj1228c(htrA)
CJJ: 
CJU: 
C8J_1171(htrA)
CJN: 
CJR: 
CJE1363(htrA)
CJD: 
CFF: 
CCV: 
CHA: 
CCO: 
CLA: 
Cla_0779(htrA)
ABU: 
Abu_2099(htrA)
ANT: 
SDL: 
NIS: 
NAM: 
RHI: 
RET: 
RLE: 
BJA: 
blr5235(degP)
BRA: 
BRADO2695(degP)
BBT: 
BBta_3032(degP)
 » show all
Taxonomy
Reference
1  [PMID:2180903]
  Authors
Lipinska B, Zylicz M, Georgopoulos C.
  Title
The HtrA (DegP) protein, essential for Escherichia coli survival at high temperatures, is an endopeptidase.
  Journal
J. Bacteriol. 172 (1990) 1791-7.
  Sequence
[eco:b0161]
Reference
2  [PMID:2025286]
  Authors
Seol JH, Woo SK, Jung EM, Yoo SJ, Lee CS, Kim KJ, Tanaka K, Ichihara A, Ha DB, Chung CH.
  Title
Protease Do is essential for survival of Escherichia coli at high temperatures: its identity with the htrA gene product.
  Journal
Biochem. Biophys. Res. Commun. 176 (1991) 730-6.
  Sequence
[eco:b0161]
Reference
3  [PMID:12270835]
  Authors
Jones CH, Dexter P, Evans AK, Liu C, Hultgren SJ, Hruby DE.
  Title
Escherichia coli DegP protease cleaves between paired hydrophobic residues in a natural substrate: the PapA pilin.
  Journal
J. Bacteriol. 184 (2002) 5762-71.
  Sequence
[eco:b0161]
Reference
4  [PMID:6347072]
  Authors
Swamy KH, Chung CH, Goldberg AL.
  Title
Isolation and characterization of protease do from Escherichia coli, a large serine protease containing multiple subunits.
  Journal
Arch. Biochem. Biophys. 224 (1983) 543-54.
Reference
5  [PMID:9383148]
  Authors
Pallen MJ, Wren BW.
  Title
The HtrA family of serine proteases.
  Journal
Mol. Microbiol. 26 (1997) 209-21.
Reference
6  [PMID:11919638]
  Authors
Krojer T, Garrido-Franco M, Huber R, Ehrmann M, Clausen T.
  Title
Crystal structure of DegP (HtrA) reveals a new protease-chaperone machine.
  Journal
Nature. 416 (2002) 455-9.
  Sequence
[eco:b0161]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
161108-11-8

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