KEGG   ENZYME: 3.4.24.27Help
Entry
EC 3.4.24.27                Enzyme                                 

Name
thermolysin;
Bacillus thermoproteolyticus neutral proteinase;
thermoase;
thermoase Y10;
TLN
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preferential cleavage: !Leu > !Phe
Comment
A thermostable extracellular metalloendopeptidase containing four calcium ions. Enzymes that may be species variants of thermolysin are reported from Micrococcus caseolyticus [4] and Aspergillus oryzae [5]. Type example of peptidase family M4. Closely related but distinct enzymes are aeromonolysin, pseudolysin, bacillolysin, aureolysin and mycolysin
Orthology
K08603  
thermolysin
Genes
BAN: 
BAR: 
BAT: 
BCY: 
BWE: 
GKA: 
ESI: 
EAT: 
FRA: 
HAU: 
Taxonomy
Reference
1  [PMID:5954368]
  Authors
Ohta Y, Ogura Y, Wada A.
  Title
Thermostable protease from thermophilic bacteria. I. Thermostability, physiocochemical properties, and amino acid composition.
  Journal
J. Biol. Chem. 241 (1966) 5919-25.
  Organism
Bacillus thermoproteolyticus
Reference
2  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch. Biochem. Biophys. 123 (1968) 572-88.
Reference
3  [PMID:5823940]
  Authors
Latt SA, Holmquist B, Vallee BL.
  Title
Thermolysin: a zinc metalloenzyme.
  Journal
Biochem. Biophys. Res. Commun. 37 (1969) 333-9.
  Organism
Bacillus thermoproteolyticus
Reference
4  [PMID:5551628]
  Authors
Desmazeaud MJ, Hermier JH.
  Title
[Specificity of the nuclear protease produced by Micrococcus caseolyticus]
  Journal
Eur. J. Biochem. 19 (1971) 51-5.
  Organism
Micrococcus caseolyticus
Reference
5  [PMID:5004124]
  Authors
Morihara K, Tsuzuki H.
  Title
Comparative study of various neutral proteinases from microorganisms: specificity with oligopeptides.
  Journal
Arch. Biochem. Biophys. 146 (1971) 291-6.
Reference
6
  Authors
Titani, K., Hermodson, M.
  Title
A., Ericson, L. H., Walsh, K. A. and Neurath, H. Amino-acid sequence of thermolysin.
  Journal
Nature New Biol. 238 (1972) 35-37.
Reference
7
  Authors
Matthews, B.
  Title
W. Structural basis of the action of thermolysin and related zinc peptidases.
  Journal
Acc. Chem. Res. 21 (1988) 333-340.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9073-78-3

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