KEGG   ENZYME: 3.4.24.28Help
Entry
EC 3.4.24.28                Enzyme                                 
Name
bacillolysin;
Bacillus metalloendopeptidase;
Bacillus subtilis neutral proteinase;
anilozyme P 10;
Bacillus metalloproteinase;
Bacillus neutral proteinase;
megateriopeptidase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Similar, but not identical, to that of thermolysin
Comment
Variants of this enzyme have been found in species of Bacillus including B. subtilis [1,6], B. amyloliquefaciens [5], B. megaterium (megateriopeptidase, [2]), B. mesentericus [10], B. cereus [3,8,9] and B. stearothermophilus [7]. In peptidase family M4 (thermolysin family). Formerly included in EC 3.4.24.4
Orthology
K01400  
bacillolysin
Genes
BMA: 
BMV: 
BML: 
BMN: 
BPS: 
BPM: 
BPL: 
BPD: 
BTE: 
BUR: 
BCN: 
Bcen_3302
BCM: 
Bcenmc03_5219
BCJ: 
BCAM2307(zmpB)
BAC: 
BamMC406_5000
BGD: 
bgla_2g25190
BSU: 
BSU14700(nprE)
BSR: 
I33_1652
BSS: 
BSUW23_07570(nprE)
BST: 
GYO_1812
BSN: 
BSn5_19435
BSQ: 
B657_14700(nprE)
BAO: 
BAMF_1546(nprE)
BAY: 
RBAM_014550(nprE)
BAQ: 
BACAU_1430(npr)
BYA: 
BANAU_1393(npr)
BAZ: 
BAMTA208_09795(nprE)
BQL: 
LL3_01578(nprE)
BXH: 
BAXH7_01996(npr)
BQY: 
MUS_1567(nprE)
BAE: 
BATR1942_05125
BAH: 
BAMEG_2413
BAI: 
BAA_2244
BAL: 
BACI_c25380(nprE1)
BCE: 
BC0602 BC2167 BC2506 BC2735 BC3383 BC5036
BCA: 
BCE_2762
BCZ: 
BCZK0511(npr) BCZK1978 BCZK2310 BCZK2464(nprE) BCZK3091(npr) pE33L466_0100(npr) pE33L466_0375(npr) pE33L466_0380(npr)
BCR: 
BCAH187_A2324 BCAH187_A2624 BCAH187_C0042
BCB: 
BCB4264_A2189 BCB4264_A2534
BCU: 
BCAH820_2581
BCG: 
BCG9842_B2789 BCG9842_B3130
BCQ: 
BCQ_2150 BCQ_2445(npr) BCQ_PI139
BCX: 
BCA_2268 BCA_3311
BNC: 
BCN_P033
BCF: 
bcf_15970
BCY: 
Bcer98_1599 Bcer98_1621
BTK: 
BT9727_0509(npr) BT9727_1996 BT9727_2348(npr) BT9727_2499(nprE) BT9727_3170(npr)
BTL: 
BALH_0539(nprE) BALH_1941 BALH_2309 BALH_2452(nprE) BALH_2915(npr)
BTB: 
BMB171_C1948 BMB171_C2266
BWE: 
BcerKBAB4_2017
BJS: 
MY9_1610
HHD: 
HBHAL_4829(npr4)
NCA: 
Noca_3166
LAN: 
Lacal_0987
 » show all
Taxonomy
Reference
1  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch. Biochem. Biophys. 123 (1968) 572-88.
Reference
2  [PMID:4980359]
  Authors
Millet J, Acher R.
  Title
[Specificity of megateriopeptidase: an amino-endopeptidase with hydrophobic characteristics]
  Journal
Eur. J. Biochem. 9 (1969) 456-62.
Reference
3  [PMID:5002444]
  Authors
Feder J, Keay L, Garrett LR, Cirulis N, Moseley MH, Wildi BS.
  Title
Bacillus cereus neutral protease.
  Journal
Biochim. Biophys. Acta. 251 (1971) 74-8.
  Organism
Bacillus cereus
Reference
4  [PMID:2276]
  Authors
Holmquist B, Vallee BL.
  Title
Esterase activity of zinc neutral proteases.
  Journal
Biochemistry. 15 (1976) 101-7.
  Organism
Bacillus subtilis [GN:bsu]
Reference
5  [PMID:6090391]
  Authors
Vasantha N, Thompson LD, Rhodes C, Banner C, Nagle J, Filpula D.
  Title
Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.
  Journal
J. Bacteriol. 159 (1984) 811-9.
  Organism
Bacillus amyloliquefaciens [GN:bay]
Reference
6  [PMID:6090407]
  Authors
Yang MY, Ferrari E, Henner DJ.
  Title
Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.
  Journal
J. Bacteriol. 160 (1984) 15-21.
  Organism
Bacillus subtilis [GN:bsu]
Reference
7  [PMID:2993245]
  Authors
Takagi M, Imanaka T, Aiba S.
  Title
Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus.
  Journal
J. Bacteriol. 163 (1985) 824-31.
  Organism
Bacillus stearothermophilus
Reference
8  [PMID:3092843]
  Authors
Sidler W, Niederer E, Suter F, Zuber H.
  Title
The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase.
  Journal
Biol. Chem. Hoppe. Seyler. 367 (1986) 643-57.
  Organism
Bacillus subtilis [GN:bsu]
Reference
9  [PMID:3127592]
  Authors
Pauptit RA, Karlsson R, Picot D, Jenkins JA, Niklaus-Reimer AS, Jansonius JN.
  Title
Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin.
  Journal
J. Mol. Biol. 199 (1988) 525-37.
  Organism
Bacillus cereus
Reference
10 [PMID:2302386]
  Authors
Stoeva S, Kleinschmidt T, Mesrob B, Braunitzer G.
  Title
Primary structure of a zinc protease from Bacillus mesentericus strain 76.
  Journal
Biochemistry. 29 (1990) 527-34.
  Organism
Bacillus mesentericus
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9080-56-2
DBGET integrated database retrieval system