KEGG   ENZYME: 3.4.24.28Help
Entry
EC 3.4.24.28                Enzyme                                 

Name
bacillolysin;
Bacillus metalloendopeptidase;
Bacillus subtilis neutral proteinase;
anilozyme P 10;
Bacillus metalloproteinase;
Bacillus neutral proteinase;
megateriopeptidase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Similar, but not identical, to that of thermolysin
Comment
Variants of this enzyme have been found in species of Bacillus including B. subtilis [1,6], B. amyloliquefaciens [5], B. megaterium (megateriopeptidase, [2]), B. mesentericus [10], B. cereus [3,8,9] and B. stearothermophilus [7]. In peptidase family M4 (thermolysin family). Formerly included in EC 3.4.24.4
History
EC 3.4.24.28 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.28
Orthology
K01400  
bacillolysin
Genes
BMA: 
BMV: 
BML: 
BMN: 
BPS: 
BPM: 
BPL: 
BPD: 
BTE: 
BUR: 
BCN: 
BCH: 
BCM: 
BCJ: 
BCAM2307(zmpB)
BAC: 
BGD: 
BSU: 
BSU14700(nprE)
BSR: 
BSS: 
BST: 
BSN: 
BSQ: 
BAO: 
BAMF_1546(nprE)
BAY: 
BAQ: 
BYA: 
BAZ: 
BQL: 
LL3_01578(nprE)
BXH: 
BQY: 
MUS_1567(nprE)
BAE: 
BAH: 
BAI: 
BAL: 
BCE: 
BCA: 
BCZ: 
BCR: 
BCB: 
BCU: 
BCG: 
BCQ: 
BCX: 
BNC: 
BCF: 
BCY: 
BTK: 
BTL: 
BTB: 
BTHT: 
BWE: 
BJS: 
HHD: 
NCA: 
LAN: 
 » show all
Taxonomy
Reference
1  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch. Biochem. Biophys. 123 (1968) 572-88.
Reference
2  [PMID:4980359]
  Authors
Millet J, Acher R.
  Title
[Specificity of megateriopeptidase: an amino-endopeptidase with hydrophobic characteristics]
  Journal
Eur. J. Biochem. 9 (1969) 456-62.
Reference
3  [PMID:5002444]
  Authors
Feder J, Keay L, Garrett LR, Cirulis N, Moseley MH, Wildi BS.
  Title
Bacillus cereus neutral protease.
  Journal
Biochim. Biophys. Acta. 251 (1971) 74-8.
Reference
4  [PMID:2276]
  Authors
Holmquist B, Vallee BL.
  Title
Esterase activity of zinc neutral proteases.
  Journal
Biochemistry. 15 (1976) 101-7.
Reference
5  [PMID:6090391]
  Authors
Vasantha N, Thompson LD, Rhodes C, Banner C, Nagle J, Filpula D.
  Title
Genes for alkaline protease and neutral protease from Bacillus amyloliquefaciens contain a large open reading frame between the regions coding for signal sequence and mature protein.
  Journal
J. Bacteriol. 159 (1984) 811-9.
  Sequence
[bao:BAMF_1546]
Reference
6  [PMID:6090407]
  Authors
Yang MY, Ferrari E, Henner DJ.
  Title
Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation.
  Journal
J. Bacteriol. 160 (1984) 15-21.
  Sequence
[bsu:BSU14700]
Reference
7  [PMID:2993245]
  Authors
Takagi M, Imanaka T, Aiba S.
  Title
Nucleotide sequence and promoter region for the neutral protease gene from Bacillus stearothermophilus.
  Journal
J. Bacteriol. 163 (1985) 824-31.
Reference
8  [PMID:3092843]
  Authors
Sidler W, Niederer E, Suter F, Zuber H.
  Title
The primary structure of Bacillus cereus neutral proteinase and comparison with thermolysin and Bacillus subtilis neutral proteinase.
  Journal
Biol. Chem. Hoppe. Seyler. 367 (1986) 643-57.
  Sequence
Reference
9  [PMID:3127592]
  Authors
Pauptit RA, Karlsson R, Picot D, Jenkins JA, Niklaus-Reimer AS, Jansonius JN.
  Title
Crystal structure of neutral protease from Bacillus cereus refined at 3.0 A resolution and comparison with the homologous but more thermostable enzyme thermolysin.
  Journal
J. Mol. Biol. 199 (1988) 525-37.
  Sequence
Reference
10 [PMID:2302386]
  Authors
Stoeva S, Kleinschmidt T, Mesrob B, Braunitzer G.
  Title
Primary structure of a zinc protease from Bacillus mesentericus strain 76.
  Journal
Biochemistry. 29 (1990) 527-34.
  Sequence
[up:P68734]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9080-56-2

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