KEGG   ORTHOLOGY: K00320Help
Entry
K00320                      KO                                     

Name
mer
Definition
5,10-methylenetetrahydromethanopterin reductase [EC:1.5.98.2]
Pathway
Methane metabolism
Carbon metabolism
Module
M00567  
Methanogenesis, CO2 => methane
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01200 Carbon metabolism
    K00320  mer; 5,10-methylenetetrahydromethanopterin reductase
  Energy metabolism
   00680 Methane metabolism
    K00320  mer; 5,10-methylenetetrahydromethanopterin reductase
KEGG modules [BR:ko00002]
 Pathway module
  Energy metabolism
   Methane metabolism
    M00567  Methanogenesis, CO2 => methane
     K00320  mer; 5,10-methylenetetrahydromethanopterin reductase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.5  Acting on the CH-NH group of donors
   1.5.98  
    1.5.98.2  5,10-methylenetetrahydromethanopterin reductase
     K00320  mer; 5,10-methylenetetrahydromethanopterin reductase
BRITE hierarchy
Other DBs
RN: 
COG: 
GO: 
Genes
PSPW: 
BIC: 
RPB: 
SWI: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MGO: 
AFA91_27215
MVQ: 
MYVA_2357
SVL: 
Strvi_3235
SRC: 
M271_09195
SCW: 
TU94_30975
STRC: 
AA958_24575
SPRI: 
SPRI_6783
SLE: 
sle_06190(sle_06190)
SCLF: 
BB341_28080
MCW: 
A8L33_00440
BCV: 
Bcav_0157 Bcav_1677
NCA: 
Noca_1630
PSIM: 
KR76_07600
MMAR: 
MODMU_5441
PHH: 
AFB00_19275
SESP: 
BN6_46520
MAU: 
Micau_1979
MIL: 
ML5_2085
VMA: 
VAB18032_19125
ABAT: 
CFX1CAM_1029
CAP: 
CLDAP_07980 CLDAP_15430
MJA: 
MJ_1534
MFE: 
Mefer_1535
MVU: 
Metvu_0160
MFS: 
MFS40622_0752
MIF: 
Metin_0159
MJH: 
JH146_0139
MIG: 
Metig_1293
MMP: 
MMP0058(mer)
MMQ: 
MmarC5_1619
MMX: 
MmarC6_0892
MMZ: 
MmarC7_1055
MMD: 
GYY_00295
MMAK: 
MMKA1_00600(mer)
MMAO: 
MMOS7_00840(mer)
MAE: 
Maeo_0749
MVN: 
Mevan_1073
MVO: 
Mvol_1374
MOK: 
Metok_0200
MAC: 
MA_3733(mer)
MBA: 
Mbar_A0254
MBY: 
MSBRM_2698
MBW: 
MSBRW_0741
MBAR: 
MSBR2_2654
MBAK: 
MSBR3_0245 MSBR3_2771
MMA: 
MM_0628
MMJ: 
MSMAS_0681
MMAC: 
MSMAC_0682
MVC: 
MSVAZ_0679
MEK: 
MSKOL_0657
MLS: 
MSLAZ_2235
METM: 
MSMTP_0746
MEF: 
MSWH1_0878
MEQ: 
MSWHS_0937
MSJ: 
MSSAC_3543
MSZ: 
MSSIH_3084
MSW: 
MSSIT_3142
MTHE: 
MSTHC_0892
MTHR: 
MSTHT_2388
MHOR: 
MSHOH_0799
MBU: 
Mbur_2372(mer)
MMET: 
MCMEM_0517
MMH: 
Mmah_1513
MHAZ: 
BHR79_06165
MEV: 
Metev_2139
MZH: 
Mzhil_0168
MPY: 
Mpsy_2035
MHZ: 
Metho_2247
MTP: 
Mthe_0205
MCJ: 
MCON_2394(mer)
MHI: 
Mhar_0856
MHU: 
Mhun_2257
MLA: 
Mlab_0076
MEM: 
Memar_0557
MBG: 
BN140_1674(mer)
MEMA: 
MMAB1_2155(mer)
MPI: 
Mpet_2180
MBN: 
Mboo_0528
MFO: 
Metfor_2659
MPL: 
Mpal_0327
MPD: 
MCP_2681(mer)
MEZ: 
Mtc_0135(mer)
RCI: 
RCIX564(mer)
MTH: 
MTH_1752
MMG: 
MTBMA_c03270(mer)
METC: 
MTCT_1602
MWO: 
MWSIV6_0292(mer)
MST: 
Msp_1128(mer)
MSI: 
Msm_0542
MRU: 
mru_0569(mer)
MEB: 
Abm4_1417(mer)
MMIL: 
sm9_0419(mer)
MEYE: 
TL18_02180
MOL: 
YLM1_0233
MEL: 
Metbo_0510
MEW: 
MSWAN_1889
METH: 
MBMB1_1520(mer)
MFC: 
BRM9_0745(mer)
MFI: 
DSM1535_0704(mer)
MCUB: 
MCBB_1769(mer)
MFV: 
Mfer_0274
MKA: 
MK0524(mer)
AFU: 
AF_1066
AFG: 
AFULGI_00011650
APO: 
Arcpr_1585
AVE: 
Arcve_0808 Arcve_1055
AST: 
Asulf_00466 Asulf_02156
FPL: 
Ferp_0743
GAC: 
GACE_0389
GAH: 
GAH_01105
HDL: 
HALDL1_03000
HHB: 
Hhub_1106(mer)
HJE: 
HacjB3_13295
HMA: 
rrnAC2437(mer1)
HHI: 
HAH_2870(mer3)
HHN: 
HISP_14590
HAB: 
SG26_06730
NPH: 
NP_1056A(mer)
NMO: 
Nmlp_1133(mer)
HUT: 
Huta_1395
HTI: 
HTIA_1288
HMU: 
Hmuk_0341
HALI: 
BV210_10785
HSU: 
HLASF_0416(mer)
HSF: 
HLASA_0415(mer)
HWA: 
HQ_3154A(mer)
HWC: 
Hqrw_3697(mer)
HVO: 
HVO_1937(mer)
HME: 
HFX_2030(mer)
HGI: 
ABY42_09440
HBO: 
Hbor_05330
HLA: 
Hlac_1680
HTU: 
Htur_3573
HDA: 
BB347_11940
NMG: 
Nmag_1582
HXA: 
Halxa_3407
NAT: 
NJ7G_2905
NPE: 
Natpe_0251
NGE: 
Natgr_2297
HRU: 
Halru_2719
NOU: 
Natoc_2482
SALI: 
L593_00135
HLR: 
HALLA_18435
HLC: 
CHINAEXTREME06980
CSU: 
CSUB_C0377 CSUB_C0386 CSUB_C0571 CSUB_C0806 CSUB_C1220 CSUB_C1712
BARC: 
AOA65_0782(mer_2)
HAH: 
Halar_1772
 » show all
TaxonomyKoalaUniProt
Reference
PMID:2298726
  Authors
te Brommelstroet BW, Hensgens CM, Keltjens JT, van der Drift C, Vogels GD
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain  delta H.
  Journal
J Biol Chem 265:1852-7 (1990)
  Sequence
[mth:MTH_1752]
Reference
PMID:2379499
  Authors
Ma K, Thauer RK
  Title
Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg).
  Journal
Eur J Biochem 191:187-93 (1990)
DOI:10.1111/j.1432-1033.1990.tb19109.x
  Sequence

KEGG   ENZYME: 1.5.98.2Help
Entry
EC 1.5.98.2                 Enzyme                                 

Name
5,10-methylenetetrahydromethanopterin reductase;
5,10-methylenetetrahydromethanopterin cyclohydrolase;
N5,N10-methylenetetrahydromethanopterin reductase;
methylene-H4MPT reductase;
coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase;
N5,N10-methylenetetrahydromethanopterin:coenzyme-F420 oxidoreductase
Class
Oxidoreductases;
Acting on the CH-NH group of donors
BRITE hierarchy
Sysname
5-methyltetrahydromethanopterin:coenzyme-F420 oxidoreductase
Reaction(IUBMB)
5-methyltetrahydromethanopterin + oxidized coenzyme F420 = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420 [RN:R04464]
Reaction(KEGG)
Substrate
5-methyltetrahydromethanopterin [CPD:C04488];
oxidized coenzyme F420 [CPD:C00876]
Product
5,10-methylenetetrahydromethanopterin [CPD:C04377];
reduced coenzyme F420 [CPD:C01080]
Comment
Catalyses an intermediate step in methanogenesis from CO2 and H2 in methanogenic archaea.
History
EC 1.5.98.2 created 2000 as EC 1.5.99.11, modified 2004, transferred to EC 1.5.98.2 2014
Pathway
Methane metabolism
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K00320  
5,10-methylenetetrahydromethanopterin reductase
Genes
PSPW: 
BIC: 
RPB: 
SWI: 
MSM: 
MSG: 
MSB: 
MSN: 
MSH: 
MSA: 
MGO: 
AFA91_27215
MVQ: 
MYVA_2357
SVL: 
Strvi_3235
SRC: 
M271_09195
SCW: 
TU94_30975
STRC: 
AA958_24575
SPRI: 
SPRI_6783
SLE: 
sle_06190(sle_06190)
SCLF: 
BB341_28080
MCW: 
A8L33_00440
BCV: 
Bcav_0157 Bcav_1677
NCA: 
Noca_1630
PSIM: 
KR76_07600
MMAR: 
MODMU_5441
PHH: 
AFB00_19275
SESP: 
BN6_46520
MAU: 
Micau_1979
MIL: 
ML5_2085
VMA: 
VAB18032_19125
ABAT: 
CFX1CAM_1029
CAP: 
CLDAP_07980 CLDAP_15430
MJA: 
MJ_1534
MFE: 
Mefer_1535
MVU: 
Metvu_0160
MFS: 
MFS40622_0752
MIF: 
Metin_0159
MJH: 
JH146_0139
MIG: 
Metig_1293
MMP: 
MMP0058(mer)
MMQ: 
MmarC5_1619
MMX: 
MmarC6_0892
MMZ: 
MmarC7_1055
MMD: 
GYY_00295
MMAK: 
MMKA1_00600(mer)
MMAO: 
MMOS7_00840(mer)
MAE: 
Maeo_0749
MVN: 
Mevan_1073
MVO: 
Mvol_1374
MOK: 
Metok_0200
MAC: 
MA_3733(mer)
MBA: 
Mbar_A0254
MBY: 
MSBRM_2698
MBW: 
MSBRW_0741
MBAR: 
MSBR2_2654
MBAK: 
MSBR3_0245 MSBR3_2771
MMA: 
MM_0628
MMJ: 
MSMAS_0681
MMAC: 
MSMAC_0682
MVC: 
MSVAZ_0679
MEK: 
MSKOL_0657
MLS: 
MSLAZ_2235
METM: 
MSMTP_0746
MEF: 
MSWH1_0878
MEQ: 
MSWHS_0937
MSJ: 
MSSAC_3543
MSZ: 
MSSIH_3084
MSW: 
MSSIT_3142
MTHE: 
MSTHC_0892
MTHR: 
MSTHT_2388
MHOR: 
MSHOH_0799
MBU: 
Mbur_2372(mer)
MMET: 
MCMEM_0517
MMH: 
Mmah_1513
MHAZ: 
BHR79_06165
MEV: 
Metev_2139
MZH: 
Mzhil_0168
MPY: 
Mpsy_2035
MHZ: 
Metho_2247
MTP: 
Mthe_0205
MCJ: 
MCON_2394(mer)
MHI: 
Mhar_0856
MHU: 
Mhun_2257
MLA: 
Mlab_0076
MEM: 
Memar_0557
MBG: 
BN140_1674(mer)
MEMA: 
MMAB1_2155(mer)
MPI: 
Mpet_2180
MBN: 
Mboo_0528
MFO: 
Metfor_2659
MPL: 
Mpal_0327
MPD: 
MCP_2681(mer)
MEZ: 
Mtc_0135(mer)
RCI: 
RCIX564(mer)
MTH: 
MTH_1752
MMG: 
MTBMA_c03270(mer)
METC: 
MTCT_1602
MWO: 
MWSIV6_0292(mer)
MST: 
Msp_1128(mer)
MSI: 
Msm_0542
MRU: 
mru_0569(mer)
MEB: 
Abm4_1417(mer)
MMIL: 
sm9_0419(mer)
MEYE: 
TL18_02180
MOL: 
YLM1_0233
MEL: 
Metbo_0510
MEW: 
MSWAN_1889
METH: 
MBMB1_1520(mer)
MFC: 
BRM9_0745(mer)
MFI: 
DSM1535_0704(mer)
MCUB: 
MCBB_1769(mer)
MFV: 
Mfer_0274
MKA: 
MK0524(mer)
AFU: 
AF_1066
AFG: 
AFULGI_00011650
APO: 
Arcpr_1585
AVE: 
Arcve_0808 Arcve_1055
AST: 
Asulf_00466 Asulf_02156
FPL: 
Ferp_0743
GAC: 
GACE_0389
GAH: 
GAH_01105
HDL: 
HALDL1_03000
HHB: 
Hhub_1106(mer)
HJE: 
HacjB3_13295
HMA: 
rrnAC2437(mer1)
HHI: 
HAH_2870(mer3)
HHN: 
HISP_14590
HAB: 
SG26_06730
NPH: 
NP_1056A(mer)
NMO: 
Nmlp_1133(mer)
HUT: 
Huta_1395
HTI: 
HTIA_1288
HMU: 
Hmuk_0341
HALI: 
BV210_10785
HSU: 
HLASF_0416(mer)
HSF: 
HLASA_0415(mer)
HWA: 
HQ_3154A(mer)
HWC: 
Hqrw_3697(mer)
HVO: 
HVO_1937(mer)
HME: 
HFX_2030(mer)
HGI: 
ABY42_09440
HBO: 
Hbor_05330
HLA: 
Hlac_1680
HTU: 
Htur_3573
HDA: 
BB347_11940
NMG: 
Nmag_1582
HXA: 
Halxa_3407
NAT: 
NJ7G_2905
NPE: 
Natpe_0251
NGE: 
Natgr_2297
HRU: 
Halru_2719
NOU: 
Natoc_2482
SALI: 
L593_00135
HLR: 
HALLA_18435
HLC: 
CHINAEXTREME06980
CSU: 
CSUB_C0377 CSUB_C0386 CSUB_C0571 CSUB_C0806 CSUB_C1220 CSUB_C1712
BARC: 
AOA65_0782(mer_2)
HAH: 
Halar_1772
 » show all
Taxonomy
Reference
1  [PMID:2379499]
  Authors
Ma K, Thauer RK.
  Title
Purification and properties of N5, N10-methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum (strain Marburg).
  Journal
Eur. J. Biochem. 191 (1990) 187-93.
  Sequence
Reference
2  [PMID:1911853]
  Authors
te Brommelstroet BW, Geerts WJ, Keltjens JT, van der Drift C, Vogels GD.
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin dehydrogenase and 5,10-methylenetetrahydromethanopterin reductase, two coenzyme F420-dependent enzymes, from Methanosarcina barkeri.
  Journal
Biochim. Biophys. Acta. 1079 (1991) 293-302.
Reference
3  [PMID:1696553]
  Authors
Ma K, Thauer RK.
  Title
Single step purification of methylenetetrahydromethanopterin reductase from Methanobacterium thermoautotrophicum by specific binding to blue sepharose CL-6B.
  Journal
FEBS. Lett. 268 (1990) 59-62.
Reference
4  [PMID:2298726]
  Authors
te Brommelstroet BW, Hensgens CM, Keltjens JT, van der Drift C, Vogels GD.
  Title
Purification and properties of 5,10-methylenetetrahydromethanopterin reductase, a coenzyme F420-dependent enzyme, from Methanobacterium thermoautotrophicum strain delta H.
  Journal
J. Biol. Chem. 265 (1990) 1852-7.
  Sequence
[mth:MTH_1752]
Reference
5  [PMID:2298699]
  Authors
te Brommelstroet BW, Hensgens CM, Geerts WJ, Keltjens JT, van der Drift C, Vogels GD.
  Title
Purification and properties of 5,10-methenyltetrahydromethanopterin cyclohydrolase from Methanosarcina barkeri.
  Journal
J. Bacteriol. 172 (1990) 564-71.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R04464Help
Entry
R04464                      Reaction                               

Name
5,10-Methylenetetrahydromethanopterin:coenzyme-F420 oxidoreductase
Definition
5,10-Methylenetetrahydromethanopterin + Reduced coenzyme F420 <=> 5-Methyl-5,6,7,8-tetrahydromethanopterin + Coenzyme F420
Equation
Reaction class
C04377_C04488
C00876_C01080
Enzyme
Pathway
Methane metabolism
Metabolic pathways
Microbial metabolism in diverse environments
Carbon metabolism
Module
M00567  
Methanogenesis, CO2 => methane
Orthology
K00320  
5,10-methylenetetrahydromethanopterin reductase [EC:1.5.98.2]
DBGET integrated database retrieval system