KEGG   ORTHOLOGY: K05308Help
Entry
K05308                      KO                                     

Name
gnaD
Definition
gluconate/galactonate dehydratase [EC:4.2.1.140]
Pathway
Pentose phosphate pathway
Galactose metabolism
Carbon metabolism
Module
M00308  
Semi-phosphorylative Entner-Doudoroff pathway, gluconate => glycerate-3P
M00309  
Non-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate
M00633  
Semi-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate-3P
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01200 Carbon metabolism
    K05308  gnaD; gluconate/galactonate dehydratase
  Carbohydrate metabolism
   00030 Pentose phosphate pathway
    K05308  gnaD; gluconate/galactonate dehydratase
   00052 Galactose metabolism
    K05308  gnaD; gluconate/galactonate dehydratase
KEGG modules [BR:ko00002]
 Pathway module
  Carbohydrate and lipid metabolism
   Central carbohydrate metabolism
    M00308  Semi-phosphorylative Entner-Doudoroff pathway, gluconate => glycerate-3P
     K05308  gnaD; gluconate/galactonate dehydratase
    M00633  Semi-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate-3P
     K05308  gnaD; gluconate/galactonate dehydratase
    M00309  Non-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate
     K05308  gnaD; gluconate/galactonate dehydratase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.140  gluconate/galactonate dehydratase
     K05308  gnaD; gluconate/galactonate dehydratase
BRITE hierarchy
Other DBs
Genes
HAL: 
VNG_0442G(rspA)
HSL: 
OE_1664R(gnaD)
HHB: 
Hhub_1548(gnaD1)
HJE: 
HMA: 
rrnAC0575(dgoA4)
HHI: 
HHN: 
HAB: 
NMO: 
Nmlp_1390(gnaD)
HMU: 
HWA: 
HQ_2412A(gnaD)
HWC: 
Hqrw_2676(gnaD)
HVO: 
HVO_1488(gnaD)
HME: 
HFX_1553(gnaD)
HGI: 
ABY42_07250
HBO: 
Hbor_17420
HLA: 
Hlac_1300
HTU: 
Htur_2654
NMG: 
Nmag_1145
HXA: 
Halxa_4119 Halxa_4131
NAT: 
NJ7G_1251
NPE: 
Natpe_2057
NGE: 
Natgr_0070
HRU: 
Halru_1854
NOU: 
Natoc_2547
SALI: 
L593_03520
HLR: 
HALLA_06625
TVO: 
TVG0179275(TVG0179275)
PTO: 
PTO0485
FAC: 
FACI_IFERC01G0930
SSO: 
SSO3198
SOL: 
Ssol_0935
SSOA: 
SULA_0968
SSOL: 
SULB_0970
SSOF: 
SULC_0969
STO: 
STK_23660(gnaD)
SAI: 
Saci_0885
SACN: 
SacN8_04295
SACR: 
SacRon12I_04285
SACS: 
SUSAZ_04035
SIS: 
LS215_2325
SIA: 
M1425_2162
SIM: 
M1627_2242
SID: 
M164_2165
SIY: 
YG5714_2288
SIN: 
YN1551_0627
SII: 
LD85_2428
SIH: 
SiH_2107
SIR: 
SiRe_2039
SIC: 
SiL_2014
SULA: 
BFU36_06085
MSE: 
Msed_1303
MCN: 
Mcup_0900
AHO: 
Ahos_0261
PYR: 
P186_1932
CMA: 
Cmaq_0154
TUZ: 
TUZN_0958
TTN: 
TTX_1156(gad)
VDI: 
Vdis_1648
VMO: 
VMUT_2313
HAH: 
Halar_3730
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Kim S, Lee SB
  Title
Identification and characterization of Sulfolobus solfataricus D-gluconate dehydratase: a key enzyme in the non-phosphorylated Entner-Doudoroff pathway.
  Journal
Biochem J 387:271-80 (2005)
DOI:10.1042/BJ20041053
  Sequence
[sso:SSO3198]
KEGG   ENZYME: 4.2.1.140Help
Entry
EC 4.2.1.140                Enzyme                                 

Name
gluconate/galactonate dehydratase;
gluconate dehydratase (ambiguous);
Sso3198 (gene name);
Pto0485 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
D-gluconate/D-galactonate hydro-lyase
Reaction(IUBMB)
(1) D-gluconate = 2-dehydro-3-deoxy-D-gluconate + H2O [RN:R01538];
(2) D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H2O [RN:R03033]
Reaction(KEGG)
Substrate
D-gluconate [CPD:C00257];
D-galactonate [CPD:C00880]
Product
2-dehydro-3-deoxy-D-gluconate [CPD:C00204];
H2O [CPD:C00001];
2-dehydro-3-deoxy-D-galactonate [CPD:C01216]
Comment
The enzyme is involved in glucose and galactose catabolism via the nonphosphorylative variant of the Entner-Doudoroff pathway in Picrophilus torridus [3] and via the branched variant of the Entner-Doudoroff pathway in Sulfolobus solfataricus [1,2]. In vitro it utilizes D-gluconate with 6-10 fold higher catalytic efficiency than D-galactonate [1,3]. It requires Mg2+ for activity [1,2]. cf. EC 4.2.1.6, galactonate dehydratase, and EC 4.2.1.39, gluconate dehydratase.
History
EC 4.2.1.140 created 2013
Pathway
Pentose phosphate pathway
Galactose metabolism
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K05308  
gluconate/galactonate dehydratase
Genes
HAL: 
VNG_0442G(rspA)
HSL: 
OE_1664R(gnaD)
HHB: 
Hhub_1548(gnaD1)
HJE: 
HMA: 
rrnAC0575(dgoA4)
HHI: 
HHN: 
HAB: 
NMO: 
Nmlp_1390(gnaD)
HMU: 
HWA: 
HQ_2412A(gnaD)
HWC: 
Hqrw_2676(gnaD)
HVO: 
HVO_1488(gnaD)
HME: 
HFX_1553(gnaD)
HGI: 
ABY42_07250
HBO: 
Hbor_17420
HLA: 
Hlac_1300
HTU: 
Htur_2654
NMG: 
Nmag_1145
HXA: 
Halxa_4119 Halxa_4131
NAT: 
NJ7G_1251
NPE: 
Natpe_2057
NGE: 
Natgr_0070
HRU: 
Halru_1854
NOU: 
Natoc_2547
SALI: 
L593_03520
HLR: 
HALLA_06625
TVO: 
TVG0179275(TVG0179275)
PTO: 
PTO0485
FAC: 
FACI_IFERC01G0930
SSO: 
SSO3198
SOL: 
Ssol_0935
SSOA: 
SULA_0968
SSOL: 
SULB_0970
SSOF: 
SULC_0969
STO: 
STK_23660(gnaD)
SAI: 
Saci_0885
SACN: 
SacN8_04295
SACR: 
SacRon12I_04285
SACS: 
SUSAZ_04035
SIS: 
LS215_2325
SIA: 
M1425_2162
SIM: 
M1627_2242
SID: 
M164_2165
SIY: 
YG5714_2288
SIN: 
YN1551_0627
SII: 
LD85_2428
SIH: 
SiH_2107
SIR: 
SiRe_2039
SIC: 
SiL_2014
SULA: 
BFU36_06085
MSE: 
Msed_1303
MCN: 
Mcup_0900
AHO: 
Ahos_0261
PYR: 
P186_1932
CMA: 
Cmaq_0154
TUZ: 
TUZN_0958
TTN: 
TTX_1156(gad)
VDI: 
Vdis_1648
VMO: 
VMUT_2313
HAH: 
Halar_3730
 » show all
Taxonomy
Reference
1  [PMID:15474024]
  Authors
Lamble HJ, Milburn CC, Taylor GL, Hough DW, Danson MJ
  Title
Gluconate dehydratase from the promiscuous Entner-Doudoroff pathway in Sulfolobus solfataricus.
  Journal
FEBS. Lett. 576 (2004) 133-6.
  Sequence
[sso:SSO3198]
Reference
2  [PMID:15869466]
  Authors
Ahmed H, Ettema TJ, Tjaden B, Geerling AC, van der Oost J, Siebers B
  Title
The semi-phosphorylative Entner-Doudoroff pathway in hyperthermophilic archaea: a re-evaluation.
  Journal
Biochem. J. 390 (2005) 529-40.
  Sequence
[sso:SSO3198]
Reference
3  [PMID:20023024]
  Authors
Reher M, Fuhrer T, Bott M, Schonheit P
  Title
The nonphosphorylative Entner-Doudoroff pathway in the thermoacidophilic euryarchaeon Picrophilus torridus involves a novel 2-keto-3-deoxygluconate- specific aldolase.
  Journal
J. Bacteriol. 192 (2010) 964-74.
  Sequence
[pto:PTO0485]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R01538Help
Entry
R01538                      Reaction                               

Name
D-gluconate hydro-lyase (2-dehydro-3-deoxy-D-gluconate-forming)
Definition
D-Gluconic acid <=> 2-Dehydro-3-deoxy-D-gluconate + H2O
Equation
Reaction class
C00204_C00257
Enzyme
4.2.1.39        4.2.1.140
Pathway
Pentose phosphate pathway
Metabolic pathways
Microbial metabolism in diverse environments
Carbon metabolism
Module
M00308  
Semi-phosphorylative Entner-Doudoroff pathway, gluconate => glycerate-3P
M00309  
Non-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate
M00633  
Semi-phosphorylative Entner-Doudoroff pathway, gluconate/galactonate => glycerate-3P
Orthology
K05308  
gluconate/galactonate dehydratase [EC:4.2.1.140]
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