KEGG   ORTHOLOGY: K05525Help
Entry
K05525                      KO                                     

Name
linC, CYP111A
Definition
linalool 8-monooxygenase [EC:1.14.13.151]
Pathway
Monoterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K05525  linC, CYP111A; linalool 8-monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.151  linalool 8-monooxygenase
     K05525  linC, CYP111A; linalool 8-monooxygenase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, bacteria type
  CYP111 family
   K05525  linC, CYP111A; linalool 8-monooxygenase
BRITE hierarchy
Other DBs
Genes
ACI: 
ACIAD1575(linC)
SUR: 
PLA: 
NAR: 
NRE: 
SMY: 
AG: 
AAA25810(linC)
TaxonomyKoalaUniProt
Reference
PMID:8376348
  Authors
Ropp JD, Gunsalus IC, Sligar SG
  Title
Cloning and expression of a member of a new cytochrome P-450 family: cytochrome P-450lin (CYP111) from Pseudomonas incognita.
  Journal
J Bacteriol 175:6028-37 (1993)
DOI:10.1128/JB.175.18.6028-6037.1993
  Sequence

KEGG   ENZYME: 1.14.13.151Help
Entry
EC 1.14.13.151              Enzyme                                 

Name
linalool 8-monooxygenase;
P-450lin;
CYP111
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
linalool,NADH:oxygen oxidoreductase (8-hydroxylating)
Reaction(IUBMB)
linalool + 2 NADH + 2 H+ + 2 O2 = (6E)-8-oxolinalool + 2 NAD+ + 3 H2O (overall reaction) [RN:R09925];
(1a) linalool + NADH + H+ + O2 = (6E)-8-hydroxylinalool + NAD+ + H2O [RN:R04366];
(1b) (6E)-8-hydroxylinalool + NADH + H+ + O2 = (6E)-8-oxolinalool + NAD+ + 2 H2O [RN:R09924]
Reaction(KEGG)
Substrate
linalool [CPD:C03985];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007];
(6E)-8-hydroxylinalool [CPD:C04433]
Product
(6E)-8-oxolinalool [CPD:C20221];
NAD+ [CPD:C00003];
H2O [CPD:C00001];
(6E)-8-hydroxylinalool [CPD:C04433]
Comment
A heme-thiolate protein (P-450). The secondary electron donor is a specific [2Fe-2S] ferredoxin from the same bacterial strain.
History
EC 1.14.13.151 created 1989 as EC 1.14.99.28, transferred 2012 to EC 1.14.13.151
Pathway
Monoterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K05525  
linalool 8-monooxygenase
Genes
ACI: 
ACIAD1575(linC)
SUR: 
PLA: 
NAR: 
NRE: 
SMY: 
Taxonomy
Reference
1  [PMID:2295633]
  Authors
Ullah AJ, Murray RI, Bhattacharyya PK, Wagner GC, Gunsalus IC
  Title
Protein components of a cytochrome P-450 linalool 8-methyl hydroxylase.
  Journal
J. Biol. Chem. 265 (1990) 1345-51.
Reference
2  [PMID:8376348]
  Authors
Ropp JD, Gunsalus IC, Sligar SG
  Title
Cloning and expression of a member of a new cytochrome P-450 family: cytochrome P-450lin (CYP111) from Pseudomonas incognita.
  Journal
J. Bacteriol. 175 (1993) 6028-37.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09923Help
Entry
R09923                      Reaction                               

Name
(-)-linalool,NADH:oxygen oxidoreductase (8-hydroxylating)
Definition
(-)-Linalool + NADH + H+ + Oxygen <=> (E)-3,7-Dimethylocta-1,6-diene-3,8-diol + NAD+ + H2O
Equation
Reaction class
C00003_C00004
C04433_C11388
Enzyme
Pathway
Monoterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K05525  
linalool 8-monooxygenase [EC:1.14.13.151]
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