KEGG   ORTHOLOGY: K08686Help
Entry
K08686                      KO                                     

Name
cbdA
Definition
2-halobenzoate 1,2-dioxygenase large subunit [EC:1.14.12.13]
Pathway
Fluorobenzoate degradation
Degradation of aromatic compounds
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K08686  cbdA; 2-halobenzoate 1,2-dioxygenase large subunit
  Xenobiotics biodegradation and metabolism
   00364 Fluorobenzoate degradation
    K08686  cbdA; 2-halobenzoate 1,2-dioxygenase large subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.13  2-halobenzoate 1,2-dioxygenase
     K08686  cbdA; 2-halobenzoate 1,2-dioxygenase large subunit
BRITE hierarchy
Other DBs
RN: 
COG: 
GO: 
Genes
AG: 
CAA55681(cbdA)
TaxonomyKoalaUniProt
Reference
PMID:7530709
  Authors
Haak B, Fetzner S, Lingens F
  Title
Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS.
  Journal
J Bacteriol 177:667-75 (1995)
DOI:10.1128/JB.177.3.667-675.1995
  Sequence

KEGG   ORTHOLOGY: K20808Help
Entry
K20808                      KO                                     

Name
cbdB
Definition
2-halobenzoate 1,2-dioxygenase small subunit [EC:1.14.12.13]
Pathway
Fluorobenzoate degradation
Degradation of aromatic compounds
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K20808  cbdB; 2-halobenzoate 1,2-dioxygenase small subunit
  Xenobiotics biodegradation and metabolism
   00364 Fluorobenzoate degradation
    K20808  cbdB; 2-halobenzoate 1,2-dioxygenase small subunit
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.13  2-halobenzoate 1,2-dioxygenase
     K20808  cbdB; 2-halobenzoate 1,2-dioxygenase small subunit
BRITE hierarchy
Other DBs
RN: 
GO: 
Genes
AG: 
CAA55682(cbdB)
TaxonomyKoalaUniProt
Reference
PMID:7530709
  Authors
Haak B, Fetzner S, Lingens F
  Title
Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS.
  Journal
J Bacteriol 177:667-75 (1995)
DOI:10.1128/JB.177.3.667-675.1995
  Sequence

KEGG   ORTHOLOGY: K20809Help
Entry
K20809                      KO                                     

Name
cbdC
Definition
2-halobenzoate 1,2-dioxygenase electron transfer component [EC:1.18.1.3]
Pathway
Fluorobenzoate degradation
Degradation of aromatic compounds
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K20809  cbdC; 2-halobenzoate 1,2-dioxygenase electron transfer component
  Xenobiotics biodegradation and metabolism
   00364 Fluorobenzoate degradation
    K20809  cbdC; 2-halobenzoate 1,2-dioxygenase electron transfer component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.18  Acting on iron-sulfur proteins as donors
   1.18.1  With NAD+ or NADP+ as acceptor
    1.18.1.3  ferredoxin---NAD+ reductase
     K20809  cbdC; 2-halobenzoate 1,2-dioxygenase electron transfer component
BRITE hierarchy
Other DBs
Genes
AG: 
CAA55683(cbdC)
TaxonomyKoalaUniProt
Reference
PMID:7530709
  Authors
Haak B, Fetzner S, Lingens F
  Title
Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS.
  Journal
J Bacteriol 177:667-75 (1995)
DOI:10.1128/JB.177.3.667-675.1995
  Sequence

KEGG   ENZYME: 1.14.12.13Help
Entry
EC 1.14.12.13               Enzyme                                 

Name
2-halobenzoate 1,2-dioxygenase;
2-chlorobenzoate 1,2-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
2-halobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dehalogenating, decarboxylating)
Reaction(IUBMB)
a 2-halobenzoate + NADH + H+ + O2 = catechol + a halide anion + NAD+ + CO2 [RN:R01033]
Reaction(KEGG)
Substrate
2-halobenzoate;
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
catechol [CPD:C00090];
halide anion;
NAD+ [CPD:C00003];
CO2 [CPD:C00011]
Comment
A multicomponent enzyme system composed of a dioxygenase component and an electron transfer component. The latter contains FAD. The enzyme, characterized from the bacterium Burkholderia cepacia 2CBS, has a broad substrate specificity. Substrates include 2-fluorobenzoate, 2-chlorobenzoate, 2-bromobenzoate, and 2-iodobenzoate, which are processed in this order of preference.
History
EC 1.14.12.13 created 1992, modified 2012
Pathway
Fluorobenzoate degradation
Microbial metabolism in diverse environments
Orthology
K08686  
2-halobenzoate 1,2-dioxygenase large subunit
K20808  
2-halobenzoate 1,2-dioxygenase small subunit
Reference
1  [PMID:2610934]
  Authors
Fetzner S, Muller R, Lingens F.
  Title
Degradation of 2-chlorobenzoate by Pseudomonas cepacia 2CBS.
  Journal
Biol. Chem. Hoppe. Seyler. 370 (1989) 1173-82.
Reference
2  [PMID:1370284]
  Authors
Fetzner S, Muller R, Lingens F
  Title
Purification and some properties of 2-halobenzoate 1,2-dioxygenase, a two-component enzyme system from Pseudomonas cepacia 2CBS.
  Journal
J. Bacteriol. 174 (1992) 279-90.
  Sequence
Reference
3  [PMID:7530709]
  Authors
Haak B, Fetzner S, Lingens F
  Title
Cloning, nucleotide sequence, and expression of the plasmid-encoded genes for the two-component 2-halobenzoate 1,2-dioxygenase from Pseudomonas cepacia 2CBS.
  Journal
J. Bacteriol. 177 (1995) 667-75.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
125268-83-9
KEGG   REACTION: R01033Help
Entry
R01033                      Reaction                               

Name
2-chlorobenzoate,NADH:oxygen oxidoreductase (1,2-hydroxylating, dechlorinating, decarboxylating)
Definition
2-Chlorobenzoate + Oxygen + NADH <=> Cl- + Catechol + NAD+ + CO2
Equation
Reaction class
C00003_C00004
C00090_C02357
Enzyme
Pathway
Fluorobenzoate degradation
Microbial metabolism in diverse environments
Degradation of aromatic compounds
Orthology
K08686  
2-halobenzoate 1,2-dioxygenase large subunit [EC:1.14.12.13]
K20808  
2-halobenzoate 1,2-dioxygenase small subunit [EC:1.14.12.13]
K20809  
2-halobenzoate 1,2-dioxygenase electron transfer component [EC:1.18.1.3]
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