KEGG   ORTHOLOGY: K10701Help
Entry
K10701                      KO                                     

Name
apc
Definition
acetophenone carboxylase [EC:6.4.1.8]
Pathway
Ethylbenzene degradation
Degradation of aromatic compounds
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K10701  apc; acetophenone carboxylase
  Xenobiotics biodegradation and metabolism
   00642 Ethylbenzene degradation
    K10701  apc; acetophenone carboxylase
Enzymes [BR:ko01000]
 6. Ligases
  6.4  Forming carbon-carbon bonds
   6.4.1  Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
    6.4.1.8  acetophenone carboxylase
     K10701  apc; acetophenone carboxylase
BRITE hierarchy
Other DBs
RN: 
GO: 
Genes
EBA: 
c1A100(apc4) c1A102(apc3) c1A105(apc1) c1A200(apc2) c1A97(apc5)
TaxonomyKoalaUniProt
Reference
  Authors
Jobst B, Schuhle K, Linne U, Heider J
  Title
ATP-dependent carboxylation of acetophenone by a novel type of carboxylase.
  Journal
J Bacteriol 192:1387-94 (2010)
DOI:10.1128/JB.01423-09
  Sequence

KEGG   ENZYME: 6.4.1.8Help
Entry
EC 6.4.1.8                  Enzyme                                 

Name
acetophenone carboxylase
Class
Ligases;
Forming carbon-carbon bonds;
Ligases that form carbon-carbon bonds (only sub-subclass identified to date)
BRITE hierarchy
Sysname
acetophenone:carbon-dioxide ligase (ADP-forming)
Reaction(IUBMB)
2 ATP + acetophenone + HCO3- + H2O + H+ = 2 ADP + 2 phosphate + 3-oxo-3-phenylpropanoate [RN:R05453]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
acetophenone [CPD:C07113];
HCO3- [CPD:C00288];
H2O [CPD:C00001];
H+ [CPD:C00080]
Product
ADP [CPD:C00008];
phosphate [CPD:C00009];
3-oxo-3-phenylpropanoate [CPD:C07114]
Comment
The enzyme is involved in anaerobic degradation of ethylbenzene. No activity with acetone, butanone, 4-hydroxy-acetophenone or 4-amino-acetophenone.
History
EC 6.4.1.8 created 2011
Pathway
Ethylbenzene degradation
Microbial metabolism in diverse environments
Orthology
K10701  
acetophenone carboxylase
Genes
EBA: 
c1A100(apc4) c1A102(apc3) c1A105(apc1) c1A200(apc2) c1A97(apc5)
Taxonomy
Reference
1  [PMID:20047908]
  Authors
Jobst B, Schuhle K, Linne U, Heider J
  Title
ATP-dependent carboxylation of acetophenone by a novel type of carboxylase.
  Journal
J. Bacteriol. 192 (2010) 1387-94.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R05453Help
Entry
R05453                      Reaction                               

Name
acetophenone:carbon-dioxide ligase (ADP-forming)
Definition
2 ATP + Acetophenone + HCO3- + H2O + H+ <=> 2 ADP + 2 Orthophosphate + 3-Oxo-3-phenylpropanoate
Equation
Reaction class
C00002_C00008
C07113_C07114
Enzyme
Pathway
Ethylbenzene degradation
Microbial metabolism in diverse environments
Degradation of aromatic compounds
Orthology
K10701  
acetophenone carboxylase [EC:6.4.1.8]
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