| Entry |
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| Name |
chlorophyllide a reductase;
bchX (gene name);
bchY (gene name);
bchZ (gene name);
COR
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| Class |
Oxidoreductases;
Acting on the CH-CH group of donors;
With an iron-sulfur protein as acceptor
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| Sysname |
bacteriochlorophyllide-a:ferredoxin 7,8-oxidoreductase
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| Reaction(IUBMB) |
(1) 3-deacetyl-3-vinylbacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate = chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ [RN: R09053];
(2) bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate = 3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+;
(3) 3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate = 3-devinyl-3-(1-hydroxyethyl)chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ [RN: R09060]
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| Reaction(KEGG) |
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| Substrate |
3-deacetyl-3-vinylbacteriochlorophyllide a [CPD: C18152];
oxidized ferredoxin [iron-sulfur] cluster [CPD: C00139];
ADP [CPD: C00008];
phosphate [CPD: C00009];
bacteriochlorophyllide a [CPD: C18155];
3-deacetyl-3-(1-hydroxyethyl)bacteriochlorophyllide a [CPD: C18153]
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| Product |
chlorophyllide a [CPD: C02139];
reduced ferredoxin [iron-sulfur] cluster [CPD: C00138];
ATP [CPD: C00002];
H2O [CPD: C00001];
H+ [CPD: C00080];
3-acetyl-3-devinylchlorophyllide a;
3-devinyl-3-(1-hydroxyethyl)chlorophyllide a [CPD: C18154]
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| Comment |
The enzyme, together with EC 1.1.1.396, bacteriochlorophyllide-a dehydrogenase, and EC 4.2.1.165, chlorophyllide-a 31-hydratase, is involved in the conversion of chlorophyllide a to bacteriochlorophyllide a. These enzymes can act in multiple orders, resulting in the formation of different intermediates, but the final product of the cumulative action of the three enzymes is always bacteriochlorophyllide a. This enzyme catalyses a trans-reduction of the B-ring; the product has the (7R,8R)-configuration. In addition, the enzyme has a latent activity of EC 1.3.7.13, 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (ferredoxin) [4]. The enzyme contains a [4Fe-4S] cluster, and structurally resembles the Fe protein/MoFe protein complex of nitrogenase (EC 1.18.6.1), which catalyses an ATP-driven reduction.
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| History |
EC 1.3.7.15 created 1965 as EC 1.3.99.35, modified 2012, transferred 2016 to EC 1.3.7.15
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| Pathway |
| Porphyrin and chlorophyll metabolism | | Biosynthesis of secondary metabolites |
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| Orthology |
| 3,8-divinyl chlorophyllide a/chlorophyllide a reductase subunit X | | 3,8-divinyl chlorophyllide a/chlorophyllide a reductase subunit Y | | 3,8-divinyl chlorophyllide a/chlorophyllide a reductase subunit Z |
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| Genes |
ALV: | | TVI: | | TMB: | | MPUR: | | HHA: | | HHC: | | EBS: | | PDQ: | | RAC: | | LIM: | | LIH: | | HYR: | | RGE: | | RBN: | | RDP: | | METR: | | BEB: | | AGC: | | BRA: | | BBT: | | BRS: | | AOL: | | RPA: | | RPB: | | RPC: | | RPD: | | RPE: | | RPT: | | RPX: | | BOS: | | MEX: | | MEA: | | MDI: | | MCH: | | MRD: | | MET: | | MPO: | | MOR: | | META: | | MAQU: | | MSL: | | RVA: | | BVR: | | BSB: | | BRL: | | RSP: | | RSH: | | RSQ: | | RSK: | | RCP: | | JAN: | | RDE: | | RLI: | | DSH: | | RED: | | PTP: | | RSU: | | RHM: | | RHC: | | SUAM: | | TOM: | | RMM: | | LVS: | | HBC: | | SPHI: | | SSAN: | | CIJ: | | BLAS: | | ELQ: | | PNS: | | PORL: | | AMV: | | RRU: | | RRF: | | RCE: | | RPM: | | HMO: | | RRS: | | RCA: | | CAU: | | CAG: | | CHL: | | CTM: | | GPH: | | CTE: | | CPC: | | CLZ: | | CCH: | | CPH: | | CPB: | | CLI: | | PVI: | | PLT: | | PPH: | | PAA: | | PROC: | | PRS: | | CTS: | |
» show all
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| Reference |
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| Authors |
Nomata J, Mizoguchi T, Tamiaki H, Fujita Y |
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| Title |
A second nitrogenase-like enzyme for bacteriochlorophyll biosynthesis: reconstitution of chlorophyllide a reductase with purified X-protein (BchX) and YZ-protein (BchY-BchZ) from Rhodobacter capsulatus. |
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| Journal |
J. Biol. Chem. 281 (2006) 15021-8. |
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| Sequence |
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| Reference |
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| Authors |
Tsukatani Y, Yamamoto H, Harada J, Yoshitomi T, Nomata J, Kasahara M, Mizoguchi T, Fujita Y, Tamiaki H |
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| Title |
An unexpectedly branched biosynthetic pathway for bacteriochlorophyll b capable of absorbing near-infrared light. |
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| Journal |
Sci. Rep. 3 (2013) 1217. |
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| Reference |
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| Authors |
Lange C, Kiesel S, Peters S, Virus S, Scheer H, Jahn D, Moser J |
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| Title |
Broadened Substrate Specificity of 3-Hydroxyethyl Bacteriochlorophyllide a Dehydrogenase (BchC) Indicates a New Route for the Biosynthesis of Bacteriochlorophyll a. |
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| Journal |
J. Biol. Chem. 290 (2015) 19697-709. |
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| Reference |
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| Authors |
Harada J, Mizoguchi T, Tsukatani Y, Yokono M, Tanaka A, Tamiaki H |
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| Title |
Chlorophyllide a oxidoreductase works as one of the divinyl reductases specifically involved in bacteriochlorophyll a biosynthesis. |
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| Journal |
J. Biol. Chem. 289 (2014) 12716-26. |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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