KEGG   ORTHOLOGY: K13035Help
Entry
K13035                      KO                                     

Name
NIT4
Definition
beta-cyano-L-alanine hydratase/nitrilase [EC:3.5.5.4 4.2.1.65]
Pathway
Cyanoamino acid metabolism
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of other amino acids
   00460 Cyanoamino acid metabolism
    K13035  NIT4; beta-cyano-L-alanine hydratase/nitrilase
Enzymes [BR:ko01000]
 3. Hydrolases
  3.5  Acting on carbon-nitrogen bonds, other than peptide bonds
   3.5.5  In nitriles
    3.5.5.4  cyanoalanine nitrilase
     K13035  NIT4; beta-cyano-L-alanine hydratase/nitrilase
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.1  Hydro-lyases
    4.2.1.65  3-cyanoalanine hydratase
     K13035  NIT4; beta-cyano-L-alanine hydratase/nitrilase
BRITE hierarchy
Other DBs
Genes
RSS: 
ATH: 
AT5G22300(NIT4)
ALY: 
CRB: 
EUS: 
BRP: 
103851433(NIT-T4)
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
18590263 18598346
GRA: 
105766626 105792876
GHI: 
107948779 107953166 107954122
EGR: 
104421327 104421328
GMX: 
100778110 100810230
PVU: 
PHAVU_011G096700g
VRA: 
106775914
VAR: 
108339531
CCAJ: 
109799314
MTR: 
MTR_4g052380 MTR_4g052390
CAM: 
101500877
ADU: 
107478319
AIP: 
107630108
LJA: 
Lj0g3v0205259.1(Lj0g3v0205259.1) Lj3g3v2981810.1(Lj3g3v2981810.1) Lj3g3v2981810.2(Lj3g3v2981810.2) Lj3g3v2981810.3(Lj3g3v2981810.3) Lj3g3v2981810.4(Lj3g3v2981810.4)
LANG: 
109351736(NIT4) 109353638(NIT4B)
FVE: 
101314432
PPER: 
18770202 18774569
PMUM: 
103329255 103341728
MDM: 
103407841 103424813
PXB: 
103936462 103966370
ZJU: 
107409663 107415031
CSV: 
101209360
CMO: 
103501743
RCU: 
8263319 8263320 8263321 8263322
JCU: 
105635907 105647068
POP: 
POPTR_0004s20980g POPTR_0006s22420g(POPTRDRAFT_417421) POPTR_0009s16260g(POPTRDRAFT_199891) POPTR_0016s07510g(POPTRDRAFT_835475)
VVI: 
100240765 100241354 100241359 100243086 100248203 100250098 100253338 100263621 100265384 100853652
SLY: 
101266793
SPEN: 
107004945
SOT: 
102595618
INI: 
109160194
SIND: 
105170044
BVG: 
104899831
NNU: 
104589516 104597484 104601345 104603451
OSA: 
4330077(NIT4)
DOSA: 
Os02t0635200-01(Os02g0635200)
OBR: 
102702164
BDI: 
100822856
ATS: 
F775_32076
SBI: 
8074793
ZMA: 
542093(NIT2)
SITA: 
101767363
PDA: 
103705226
EGU: 
105042779
MUS: 
103994490
ATR: 
18426973 18444517
SMO: 
SELMODRAFT_133005(NIT4-2) SELMODRAFT_171766(NIT4-1)
PPP: 
PHYPADRAFT_88254
TVS: 
TRAVEDRAFT_139011
DSQ: 
DICSQDRAFT_95867
PCO: 
PHACADRAFT_265079 PHACADRAFT_86834
SHS: 
STEHIDRAFT_117582
PSQ: 
PUNSTDRAFT_56713
FME: 
FOMMEDRAFT_140077
MRR: 
Moror_928
SCM: 
SCHCODRAFT_255433
SLA: 
SERLADRAFT_455401
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Piotrowski M, Schonfelder S, Weiler EW
  Title
The Arabidopsis thaliana isogene NIT4 and its orthologs in tobacco encode beta-cyano-L-alanine hydratase/nitrilase.
  Journal
J Biol Chem 276:2616-21 (2001)
DOI:10.1074/jbc.M007890200
  Sequence
[ath:AT5G22300]
KEGG   ENZYME: 3.5.5.4Help
Entry
EC 3.5.5.4                  Enzyme                                 

Name
cyanoalanine nitrilase;
beta-cyanoalanine nitrilase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles
BRITE hierarchy
Sysname
3-cyano-L-alanine aminohydrolase
Reaction(IUBMB)
3-cyano-L-alanine + 2 H2O = L-aspartate + NH3 (overall reaction) [RN:R00486];
(1a) 3-cyano-L-alanine + H2O = L-asparagine [RN:R01267];
(1b) L-asparagine + H2O = L-aspartate + NH3 [RN:R00485]
Reaction(KEGG)
Substrate
3-cyano-L-alanine [CPD:C02512];
H2O [CPD:C00001];
L-asparagine [CPD:C00152]
Product
L-aspartate [CPD:C00049];
NH3 [CPD:C00014];
L-asparagine [CPD:C00152]
Comment
L-Asparagine is formed as an intermediate. cf. EC 4.2.1.65, 3-cyanoalanine hydratase and EC 3.5.1.1, asparaginase.
History
EC 3.5.5.4 created 1986
Pathway
Cyanoamino acid metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K13035  
beta-cyano-L-alanine hydratase/nitrilase
Genes
RSS: 
ATH: 
AT5G22300(NIT4)
ALY: 
CRB: 
EUS: 
BRP: 
103851433(NIT-T4)
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
18590263 18598346
GRA: 
105766626 105792876
GHI: 
107948779 107953166 107954122
EGR: 
104421327 104421328
GMX: 
100778110 100810230
PVU: 
PHAVU_011G096700g
VRA: 
106775914
VAR: 
108339531
CCAJ: 
109799314
MTR: 
MTR_4g052380 MTR_4g052390
CAM: 
101500877
ADU: 
107478319
AIP: 
107630108
LJA: 
Lj0g3v0205259.1(Lj0g3v0205259.1) Lj3g3v2981810.1(Lj3g3v2981810.1) Lj3g3v2981810.2(Lj3g3v2981810.2) Lj3g3v2981810.3(Lj3g3v2981810.3) Lj3g3v2981810.4(Lj3g3v2981810.4)
LANG: 
109351736(NIT4) 109353638(NIT4B)
FVE: 
101314432
PPER: 
18770202 18774569
PMUM: 
103329255 103341728
MDM: 
103407841 103424813
PXB: 
103936462 103966370
ZJU: 
107409663 107415031
CSV: 
101209360
CMO: 
103501743
RCU: 
8263319 8263320 8263321 8263322
JCU: 
105635907 105647068
POP: 
POPTR_0004s20980g POPTR_0006s22420g(POPTRDRAFT_417421) POPTR_0009s16260g(POPTRDRAFT_199891) POPTR_0016s07510g(POPTRDRAFT_835475)
VVI: 
100240765 100241354 100241359 100243086 100248203 100250098 100253338 100263621 100265384 100853652
SLY: 
101266793
SPEN: 
107004945
SOT: 
102595618
INI: 
109160194
SIND: 
105170044
BVG: 
104899831
NNU: 
104589516 104597484 104601345 104603451
OSA: 
4330077(NIT4)
DOSA: 
Os02t0635200-01(Os02g0635200)
OBR: 
102702164
BDI: 
100822856
ATS: 
F775_32076
SBI: 
8074793
ZMA: 
542093(NIT2)
SITA: 
101767363
PDA: 
103705226
EGU: 
105042779
MUS: 
103994490
ATR: 
18426973 18444517
SMO: 
SELMODRAFT_133005(NIT4-2) SELMODRAFT_171766(NIT4-1)
PPP: 
PHYPADRAFT_88254
TVS: 
TRAVEDRAFT_139011
DSQ: 
DICSQDRAFT_95867
PCO: 
PHACADRAFT_265079 PHACADRAFT_86834
SHS: 
STEHIDRAFT_117582
PSQ: 
PUNSTDRAFT_56713
FME: 
FOMMEDRAFT_140077
MRR: 
Moror_928
SCM: 
SCHCODRAFT_255433
SLA: 
SERLADRAFT_455401
 » show all
Taxonomy
Reference
1
  Authors
Yanase, H., Sakai, T. and Tonomuro, K.
  Title
Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13.
  Journal
Agric. Biol. Chem. 47 (1983) 473-482.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
85638-44-4
KEGG   REACTION: R00486Help
Entry
R00486                      Reaction                               

Name
3-Cyano-L-alanine aminohydrolase
Definition
3-Cyano-L-alanine + 2 H2O <=> L-Aspartate + Ammonia
Equation
Reaction class
C00049_C02512
Enzyme
Pathway
Cyanoamino acid metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K13035  
beta-cyano-L-alanine hydratase/nitrilase [EC:3.5.5.4 4.2.1.65]
DBGET integrated database retrieval system