KEGG   ORTHOLOGY: K14028Help
Entry
K14028                      KO                                     

Name
mdh1, mxaF
Definition
methanol dehydrogenase (cytochrome c) subunit 1 [EC:1.1.2.7]
Pathway
Glycolysis / Gluconeogenesis
Chloroalkane and chloroalkene degradation
Methane metabolism
Carbon metabolism
Module
M00174  
Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01200 Carbon metabolism
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
  Carbohydrate metabolism
   00010 Glycolysis / Gluconeogenesis
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
  Energy metabolism
   00680 Methane metabolism
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
  Xenobiotics biodegradation and metabolism
   00625 Chloroalkane and chloroalkene degradation
    K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
KEGG modules [BR:ko00002]
 Pathway module
  Energy metabolism
   Methane metabolism
    M00174  Methane oxidation, methanotroph, methane => formaldehyde
     K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.2  With a cytochrome as acceptor
    1.1.2.7  methanol dehydrogenase (cytochrome c)
     K14028  mdh1, mxaF; methanol dehydrogenase (cytochrome c) subunit 1
BRITE hierarchy
Other DBs
Genes
DKO: 
MCA: 
MCA0779(mxaF)
MMT: 
MDN: 
MDH: 
MAH: 
MPSY: 
MEJ: 
MEC: 
VEI: 
MFA: 
Mfla_2044
MEI: 
Msip34_0734
MEP: 
MPQ_0771
MEU: 
ACJ67_10810
RPC: 
RPC_1907
RPE: 
RPE_3139
SNO: 
Snov_4188
MEX: 
Mext_4150
MEA: 
Mex_1p4538(mxaF)
MDI: 
METDI5145(mxaF)
MCH: 
Mchl_4518
MRD: 
Mrad2831_4210
MPO: 
Mpop_4632
MNO: 
Mnod_8040
MOR: 
MOC_4862(mxaF)
META: 
Y590_20685
MAQU: 
Maq22A_1p33165(mxaF)
MSL: 
Msil_0471
HDN: 
Hden_1323
HMC: 
HYPMC_0485(mxaF)
HNI: 
W911_14545
FIL: 
BN1229_v1_2515(mxaF)
FIY: 
BN1229_v1_3401(mxaF)
MSC: 
BN69_2570(mxaF)
MBRY: 
B1812_04775
MCG: 
GL4_0421
PDE: 
Pden_2993
PYE: 
A6J80_13920
GBE: 
GbCGDNIH1_0344
GBH: 
GbCGDNIH2_0344
GBC: 
GbCGDNIH3_0344
GBS: 
GbCGDNIH4_0344
ALI: 
AZOLI_p10948(moxF1)
ATI: 
AL072_18670
MIN: 
Minf_0992(gcd)
MOX: 
DAMO_0112(mxaF)
 » show all
TaxonomyKoalaUniProt
Reference
PMID:1311606
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
Reference
PMID:7656012
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence

KEGG   ORTHOLOGY: K14029Help
Entry
K14029                      KO                                     

Name
mdh2, mxaI
Definition
methanol dehydrogenase (cytochrome c) subunit 2 [EC:1.1.2.7]
Pathway
Glycolysis / Gluconeogenesis
Chloroalkane and chloroalkene degradation
Methane metabolism
Carbon metabolism
Module
M00174  
Methane oxidation, methanotroph, methane => formaldehyde
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01200 Carbon metabolism
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
  Carbohydrate metabolism
   00010 Glycolysis / Gluconeogenesis
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
  Energy metabolism
   00680 Methane metabolism
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
  Xenobiotics biodegradation and metabolism
   00625 Chloroalkane and chloroalkene degradation
    K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
KEGG modules [BR:ko00002]
 Pathway module
  Energy metabolism
   Methane metabolism
    M00174  Methane oxidation, methanotroph, methane => formaldehyde
     K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.2  With a cytochrome as acceptor
    1.1.2.7  methanol dehydrogenase (cytochrome c)
     K14029  mdh2, mxaI; methanol dehydrogenase (cytochrome c) subunit 2
BRITE hierarchy
Other DBs
Genes
DKO: 
MCA: 
MCA0782(mxaI)
MMT: 
MDN: 
MDH: 
MAH: 
MPSY: 
MEJ: 
MEC: 
VEI: 
MFA: 
Mfla_2041
MEI: 
Msip34_0737
MEP: 
MPQ_0774
MEU: 
ACJ67_10795
RPC: 
RPC_1910
RPE: 
RPE_3136
SNO: 
Snov_4191
MEX: 
Mext_4147
MEA: 
Mex_1p4535(mxaI)
MDI: 
METDI5141(mxaI)
MCH: 
Mchl_4515
MRD: 
Mrad2831_4207
MPO: 
Mpop_4629
MNO: 
Mnod_8037
MOR: 
MOC_4859
META: 
Y590_20670
MAQU: 
Maq22A_1p33180(mxaI)
MSL: 
Msil_0474
HDN: 
Hden_1320
HMC: 
HYPMC_0488(mxaI)
HNI: 
W911_14530
FIL: 
BN1229_v1_2518(mxaI)
FIY: 
BN1229_v1_3398(mxaI)
MSC: 
BN69_2573
MCG: 
GL4_0424
PDE: 
Pden_2996
PYE: 
A6J80_13905
GBE: 
GbCGDNIH1_0347
GBH: 
GbCGDNIH2_0347
GBC: 
GbCGDNIH3_0347
GBS: 
GbCGDNIH4_0347
ALI: 
AZOLI_p10951(moxI)
ATI: 
AL072_18685
MOX: 
DAMO_0115(mxaI)
 » show all
TaxonomyKoalaUniProt
Reference
PMID:1311606
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim Biophys Acta 1119:97-106 (1992)
DOI:10.1016/0167-4838(92)90240-E
Reference
PMID:7656012
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat Struct Biol 1:102-5 (1994)
DOI:10.1038/nsb0294-102
  Sequence

KEGG   ENZYME: 1.1.2.7Help
Entry
EC 1.1.2.7                  Enzyme                                 

Name
methanol dehydrogenase (cytochrome c);
methanol dehydrogenase;
MDH
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With a cytochrome as acceptor
BRITE hierarchy
Sysname
methanol:cytochrome c oxidoreductase
Reaction(IUBMB)
a primary alcohol + 2 ferricytochrome cL = an aldehyde + 2 ferrocytochrome cL + 2 H+ [RN:R10713]
Reaction(KEGG)
Substrate
primary alcohol [CPD:C00226];
ferricytochrome cL [CPD:C18233]
Product
aldehyde [CPD:C00071];
ferrocytochrome cL [CPD:C18234];
H+ [CPD:C00080]
Comment
A periplasmic quinoprotein alcohol dehydrogenase that only occurs in methylotrophic bacteria. It uses the novel specific cytochrome cL as acceptor. Acts on a wide range of primary alcohols, including ethanol, duodecanol, chloroethanol, cinnamyl alcohol, and also formaldehyde. Activity is stimulated by ammonia or methylamine. It is usually assayed with phenazine methosulfate. Like all other quinoprotein alcohol dehydrogenases it has an 8-bladed 'propeller' structure, a calcium ion bound to the PQQ in the active site and an unusual disulfide ring structure in close proximity to the PQQ. It differs from EC 1.1.2.8, alcohol dehydrogenase (cytochrome c), in having a high affinity for methanol and in having a second essential small subunit (no known function).
History
EC 1.1.2.7 created 1972 as 1.1.99.8, modified 1982, part transferred 2010 to EC 1.1.2.7
Pathway
Glycolysis / Gluconeogenesis
Chloroalkane and chloroalkene degradation
Methane metabolism
Metabolic pathways
Biosynthesis of secondary metabolites
Microbial metabolism in diverse environments
Biosynthesis of antibiotics
Orthology
K14028  
methanol dehydrogenase (cytochrome c) subunit 1
K14029  
methanol dehydrogenase (cytochrome c) subunit 2
Genes
DKO: 
MCA: 
MCA0779(mxaF) MCA0782(mxaI)
MMT: 
MDN: 
MDH: 
MAH: 
MPSY: 
MEJ: 
MEC: 
VEI: 
MFA: 
Mfla_2041 Mfla_2044
MEI: 
Msip34_0734 Msip34_0737
MEP: 
MPQ_0771 MPQ_0774
MEU: 
ACJ67_10795 ACJ67_10810
RPC: 
RPC_1907 RPC_1910
RPE: 
RPE_3136 RPE_3139
SNO: 
Snov_4188 Snov_4191
MEX: 
Mext_4147 Mext_4150
MEA: 
Mex_1p4535(mxaI) Mex_1p4538(mxaF)
MDI: 
METDI5141(mxaI) METDI5145(mxaF)
MCH: 
Mchl_4515 Mchl_4518
MRD: 
Mrad2831_4207 Mrad2831_4210
MPO: 
Mpop_4629 Mpop_4632
MNO: 
Mnod_8037 Mnod_8040
MOR: 
MOC_4859 MOC_4862(mxaF)
META: 
Y590_20670 Y590_20685
MAQU: 
Maq22A_1p33165(mxaF) Maq22A_1p33180(mxaI)
MSL: 
Msil_0471 Msil_0474
HDN: 
Hden_1320 Hden_1323
HMC: 
HYPMC_0485(mxaF) HYPMC_0488(mxaI)
HNI: 
W911_14530 W911_14545
FIL: 
BN1229_v1_2515(mxaF) BN1229_v1_2518(mxaI)
FIY: 
BN1229_v1_3398(mxaI) BN1229_v1_3401(mxaF)
MSC: 
BN69_2570(mxaF) BN69_2573
MBRY: 
B1812_04775
MCG: 
GL4_0421 GL4_0424
PDE: 
Pden_2993 Pden_2996
PYE: 
A6J80_13905 A6J80_13920
GBE: 
GbCGDNIH1_0344 GbCGDNIH1_0347
GBH: 
GbCGDNIH2_0344 GbCGDNIH2_0347
GBC: 
GbCGDNIH3_0344 GbCGDNIH3_0347
GBS: 
GbCGDNIH4_0344 GbCGDNIH4_0347
ALI: 
AZOLI_p10948(moxF1) AZOLI_p10951(moxI)
ATI: 
AL072_18670 AL072_18685
MIN: 
Minf_0992(gcd)
MOX: 
DAMO_0112(mxaF) DAMO_0115(mxaI)
 » show all
Taxonomy
Reference
1  [PMID:4378696]
  Authors
Anthony C, Zatman LJ
  Title
The microbial oxidation of methanol. 2. The methanol-oxidizing enzyme of Pseudomonas sp. M 27.
  Journal
Biochem. J. 92 (1964) 614-21.
Reference
2  [PMID:6049934]
  Authors
Anthony C, Zatman LJ
  Title
The microbial oxidation of methanol. The prosthetic group of the alcohol dehydrogenase of Pseudomonas sp. M27: a new oxidoreductase prosthetic group.
  Journal
Biochem. J. 104 (1967) 960-9.
Reference
3  [PMID:6250827]
  Authors
Duine JA, Frank J, Verwiel PE.
  Title
Structure and activity of the prosthetic group of methanol dehydrogenase.
  Journal
Eur. J. Biochem. 108 (1980) 187-92.
Reference
4  [PMID:471057]
  Authors
Salisbury SA, Forrest HS, Cruse WB, Kennard O.
  Title
A novel coenzyme from bacterial primary alcohol dehydrogenases.
  Journal
Nature. 280 (1979) 843-4.
Reference
5  [PMID:1311606]
  Authors
Cox JM, Day DJ, Anthony C
  Title
The interaction of methanol dehydrogenase and its electron acceptor, cytochrome cL in methylotrophic bacteria.
  Journal
Biochim. Biophys. Acta. 1119 (1992) 97-106.
  Sequence
Reference
6  [PMID:7656012]
  Authors
Blake CC, Ghosh M, Harlos K, Avezoux A, Anthony C
  Title
The active site of methanol dehydrogenase contains a disulphide bridge between adjacent cysteine residues.
  Journal
Nat. Struct. Biol. 1 (1994) 102-5.
  Sequence
Reference
7  [PMID:9930981]
  Authors
Xia ZX, He YN, Dai WW, White SA, Boyd GD, Mathews FS
  Title
Detailed active site configuration of a new crystal form of methanol dehydrogenase from Methylophilus W3A1 at 1.9 A resolution.
  Journal
Biochemistry. 38 (1999) 1214-20.
Reference
8  [PMID:11502173]
  Authors
Afolabi PR, Mohammed F, Amaratunga K, Majekodunmi O, Dales SL, Gill R, Thompson D, Cooper JB, Wood SP, Goodwin PM, Anthony C
  Title
Site-directed mutagenesis and X-ray crystallography of the PQQ-containing quinoprotein methanol dehydrogenase and its electron acceptor, cytochrome c(L).
  Journal
Biochemistry. 40 (2001) 9799-809.
  Sequence
Reference
9  [PMID:12686102]
  Authors
Anthony C, Williams P
  Title
The structure and mechanism of methanol dehydrogenase.
  Journal
Biochim. Biophys. Acta. 1647 (2003) 18-23.
Reference
10 [PMID:15608378]
  Authors
Williams PA, Coates L, Mohammed F, Gill R, Erskine PT, Coker A, Wood SP, Anthony C, Cooper JB
  Title
The atomic resolution structure of methanol dehydrogenase from Methylobacterium extorquens.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 61 (2005) 75-9.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
37205-43-9
KEGG   REACTION: R09127Help
Entry
R09127                      Reaction                               

Name
ethanol:cytochrome c oxidoreductase
Definition
Ethanol + 2 Ferricytochrome cL <=> Acetaldehyde + 2 Ferrocytochrome cL + 2 H+
Equation
C00469 + 2 C18233 <=> C00084 + 2 C18234 + 2 C00080
Reaction class
C00084_C00469
Enzyme
Pathway
Glycolysis / Gluconeogenesis
Metabolic pathways
Biosynthesis of secondary metabolites
Microbial metabolism in diverse environments
Biosynthesis of antibiotics
Orthology
K14028  
methanol dehydrogenase (cytochrome c) subunit 1 [EC:1.1.2.7]
K14029  
methanol dehydrogenase (cytochrome c) subunit 2 [EC:1.1.2.7]
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