KEGG   ORTHOLOGY: K14037Help
Entry
K14037                      KO                                     

Name
KSL10
Definition
ent-sandaracopimaradiene/labdatriene synthase [EC:4.2.3.29 4.2.3.99]
Pathway
Diterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K14037  KSL10; ent-sandaracopimaradiene/labdatriene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.29  ent-sandaracopimaradiene synthase
     K14037  KSL10; ent-sandaracopimaradiene/labdatriene synthase
    4.2.3.99  labdatriene synthase
     K14037  KSL10; ent-sandaracopimaradiene/labdatriene synthase
BRITE hierarchy
Other DBs
Genes
OSA: 
4352248(KS10)
DOSA: 
Os12t0491800-01(Os12g0491800)
TaxonomyKoalaUniProt
Reference
  Authors
Yajima A, Yamaguchi A, Nukada T, Yabuta G
  Title
Asymmetric total synthesis of ent-sandaracopimaradiene, a biosynthetic intermediate of oryzalexins.
  Journal
Biosci Biotechnol Biochem 71:2822-9 (2007)
DOI:10.1271/bbb.70468
Reference
  Authors
Morrone D, Hillwig ML, Mead ME, Lowry L, Fulton DB, Peters RJ
  Title
Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals.
  Journal
Biochem J 435:589-95 (2011)
DOI:10.1042/BJ20101429
KEGG   ENZYME: 4.2.3.99Help
Entry
EC 4.2.3.99                 Enzyme                                 

Name
labdatriene synthase;
OsKSL10 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
9alpha-copalyl-diphosphate diphosphate-lyase [(12E)-9alpha-labda-8(17),12,14-triene-forming]
Reaction(IUBMB)
9alpha-copalyl diphosphate = (12E)-9alpha-labda-8(17),12,14-triene + diphosphate [RN:R09899]
Reaction(KEGG)
Substrate
9alpha-copalyl diphosphate [CPD:C11902]
Product
(12E)-9alpha-labda-8(17),12,14-triene [CPD:C20185];
diphosphate [CPD:C00013]
Comment
The enzyme from rice (Oryza sativa), expressed in Escherichia coli, also produces ent-sandaracopimara-8(14),15-diene from ent-copalyl diphosphate, another naturally occuring copalyl isomer in rice (cf. ent-sandaracopimaradiene synthase, EC 4.2.3.29).
History
EC 4.2.3.99 created 2012
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K14037  
ent-sandaracopimaradiene/labdatriene synthase
Genes
OSA: 
4352248(KS10)
DOSA: 
Os12t0491800-01(Os12g0491800)
Taxonomy
Reference
1  [PMID:21323642]
  Authors
Morrone D, Hillwig ML, Mead ME, Lowry L, Fulton DB, Peters RJ
  Title
Evident and latent plasticity across the rice diterpene synthase family with potential implications for the evolution of diterpenoid metabolism in the cereals.
  Journal
Biochem. J. 435 (2011) 589-95.
  Sequence
[osa:4352248]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09899Help
Entry
R09899                      Reaction                               

Name
9alpha-copalyl-diphosphate diphosphate-lyase [(12E)-9alpha-labda-8(17),12,14-triene-forming]
Definition
9alpha-Copalyl diphosphate <=> (12E)-9alpha-Labda-8(17),12,14-triene + Diphosphate
Equation
Reaction class
C11902_C20185
Enzyme
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K14037  
ent-sandaracopimaradiene/labdatriene synthase [EC:4.2.3.29 4.2.3.99]
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