KEGG   ORTHOLOGY: K14042Help
Entry
K14042                      KO                                     

Name
TPS-ISO
Definition
isopimara-7,15-diene synthase /copalyl diphosphate synthase [EC:4.2.3.44 5.5.1.12]
Pathway
Diterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K14042  TPS-ISO; isopimara-7,15-diene synthase /copalyl diphosphate synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.44  isopimara-7,15-diene synthase
     K14042  TPS-ISO; isopimara-7,15-diene synthase /copalyl diphosphate synthase
 5. Isomerases
  5.5  Intramolecular lyases
   5.5.1  Intramolecular lyases (only sub-subclass identified to date)
    5.5.1.12  copalyl diphosphate synthase
     K14042  TPS-ISO; isopimara-7,15-diene synthase /copalyl diphosphate synthase
BRITE hierarchy
Other DBs
Genes
AG: 
AAS47690(TPS-ISO)
TaxonomyKoalaUniProt
Reference
  Authors
Martin DM, Faldt J, Bohlmann J
  Title
Functional characterization of nine Norway Spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily.
  Journal
Plant Physiol 135:1908-27 (2004)
DOI:10.1104/pp.104.042028
  Sequence
Reference
  Authors
Wilderman PR, Peters RJ
  Title
A single residue switch converts abietadiene synthase into a pimaradiene specific cyclase.
  Journal
J Am Chem Soc 129:15736-7 (2007)
DOI:10.1021/ja074977g
KEGG   ENZYME: 4.2.3.44Help
Entry
EC 4.2.3.44                 Enzyme                                 

Name
isopimara-7,15-diene synthase;
PaTPS-Iso;
copalyl diphosphate-lyase (isopimara-7,15-diene-forming)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(+)-copalyl diphosphate-lyase (isopimara-7,15-diene-forming)
Reaction(IUBMB)
(+)-copalyl diphosphate = isopimara-7,15-diene + diphosphate [RN:R09111]
Reaction(KEGG)
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
isopimara-7,15-diene [CPD:C18211];
diphosphate [CPD:C00013]
Comment
The enzyme only gave isopimara-7,15-diene.
History
EC 4.2.3.44 created 2009
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K14042  
isopimara-7,15-diene synthase /copalyl diphosphate synthase
Reference
1  [PMID:15310829]
  Authors
Martin DM, Faldt J, Bohlmann J.
  Title
Functional characterization of nine Norway Spruce TPS genes and evolution of gymnosperm terpene synthases of the TPS-d subfamily.
  Journal
Plant. Physiol. 135 (2004) 1908-27.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09111Help
Entry
R09111                      Reaction                               

Name
Copalyl diphosphate-lyase (isopimara-7,15-diene-forming)
Definition
Copalyl diphosphate <=> Isopimara-7,15-diene + Diphosphate
Equation
Reaction class
C11901_C18211
Enzyme
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K14042  
isopimara-7,15-diene synthase /copalyl diphosphate synthase [EC:4.2.3.44 5.5.1.12]
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