KEGG   ORTHOLOGY: K14182Help
Entry
K14182                      KO                                     

Name
HVS
Definition
vetispiradiene synthase [EC:4.2.3.21]
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00909 Sesquiterpenoid and triterpenoid biosynthesis
    K14182  HVS; vetispiradiene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.21  vetispiradiene synthase
     K14182  HVS; vetispiradiene synthase
BRITE hierarchy
Other DBs
RN: 
GO: 
Genes
SLY: 
101264374(TPS33) 101264676(TPS35)
SPEN: 
SOT: 
102577472(PVS2) 102595695(PVS4)
AG: 
TaxonomyKoalaUniProt
Reference
PMID:7706281
  Authors
Back K, Chappell J
  Title
Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases.
  Journal
J Biol Chem 270:7375-81 (1995)
DOI:10.1074/jbc.270.13.7375
  Sequence

KEGG   ENZYME: 4.2.3.21Help
Entry
EC 4.2.3.21                 Enzyme                                 

Name
vetispiradiene synthase;
vetispiradiene-forming farnesyl pyrophosphate cyclase;
pemnaspirodiene synthase;
HVS;
vetispiradiene cyclase
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(2E,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, vetispiradiene-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = vetispiradiene + diphosphate [RN:R06523]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
vetispiradiene [CPD:C12142];
diphosphate [CPD:C00013]
Comment
The initial internal cyclization produces the monocyclic intermediate germacrene A.
History
EC 4.2.3.21 created 2004, modified 2011
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K14182  
vetispiradiene synthase
Genes
SLY: 
101264374(TPS33) 101264676(TPS35)
SPEN: 
SOT: 
102577472(PVS2) 102595695(PVS4)
Taxonomy
Reference
1  [PMID:7706281]
  Authors
Back K, Chappell J.
  Title
Cloning and bacterial expression of a sesquiterpene cyclase from Hyoscyamus muticus and its molecular comparison to related terpene cyclases.
  Journal
J. Biol. Chem. 270 (1995) 7375-81.
  Sequence
Reference
2
  Authors
Keller, H., Czernic, P., Ponchet, M., Ducrot, P.H., Back, K., Chappell, J., Ricci, P. and Marco, Y.
  Title
Sesquiterpene cyclase is not a determining factor for elicitor- and pathogen-induced capsidiol accumulation in tobacco.
  Journal
Planta 205 (1998) 467-476.
Reference
3  [PMID:9204881]
  Authors
Mathis JR, Back K, Starks C, Noel J, Poulter CD, Chappell J.
  Title
Pre-steady-state study of recombinant sesquiterpene cyclases.
  Journal
Biochemistry. 36 (1997) 8340-8.
Reference
4  [PMID:10588069]
  Authors
Yoshioka H, Yamada N, Doke N.
  Title
cDNA cloning of sesquiterpene cyclase and squalene synthase, and expression of the genes in potato tuber infected with Phytophthora infestans.
  Journal
Plant. Cell. Physiol. 40 (1999) 993-8.
  Sequence
[sot:102595695]
Reference
5  [PMID:11745124]
  Authors
Martin VJ, Yoshikuni Y, Keasling JD.
  Title
The in vivo synthesis of plant sesquiterpenes by Escherichia coli.
  Journal
Biotechnol. Bioeng. 75 (2001) 497-503.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
192465-18-2
KEGG   REACTION: R06523Help
Entry
R06523                      Reaction                               

Name
trans,trans-farnesyl-diphosphate diphosphate-lyase (cyclizing, vetispiradiene-forming)
Definition
trans,trans-Farnesyl diphosphate <=> Vetispiradiene + Diphosphate
Equation
Reaction class
C00448_C12142
Enzyme
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K14182  
vetispiradiene synthase [EC:4.2.3.21]
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