KEGG   ORTHOLOGY: K15088Help
Entry
K15088                      KO                                     

Name
E4.2.3.16
Definition
(4S)-limonene synthase [EC:4.2.3.16]
Pathway
Monoterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00902 Monoterpenoid biosynthesis
    K15088  E4.2.3.16; (4S)-limonene synthase
Enzymes [BR:ko01000]
 4. Lyases
  4.2  Carbon-oxygen lyases
   4.2.3  Acting on phosphates
    4.2.3.16  (4S)-limonene synthase
     K15088  E4.2.3.16; (4S)-limonene synthase
BRITE hierarchy
Other DBs
RN: 
GO: 
Genes
AG: 
TaxonomyKoalaUniProt
Reference
PMID:8226816
  Authors
Colby SM, Alonso WR, Katahira EJ, McGarvey DJ, Croteau R
  Title
4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase.
  Journal
J Biol Chem 268:23016-24 (1993)
  Sequence

KEGG   ENZYME: 4.2.3.16Help
Entry
EC 4.2.3.16                 Enzyme                                 

Name
(4S)-limonene synthase;
(-)-(4S)-limonene synthase;
4S-(-)-limonene synthase;
geranyldiphosphate diphosphate lyase (limonene forming);
geranyldiphosphate diphosphate lyase [cyclizing, (4S)-limonene-forming];
geranyl-diphosphate diphosphate-lyase [cyclizing;
(-)-(4S)-limonene-forming]
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
geranyl-diphosphate diphosphate-lyase [cyclizing; (S)-limonene-forming]
Reaction(IUBMB)
geranyl diphosphate = (S)-limonene + diphosphate [RN:R02013]
Reaction(KEGG)
Substrate
geranyl diphosphate [CPD:C00341]
Product
(S)-limonene;
diphosphate [CPD:C00013]
Comment
A recombinant enzyme (also known as a monoterpene synthase or cyclase) from the grand fir (Abies grandis) requires Mn2+ and K+ for activity. Mg2+ is essentially ineffective as the divalent metal ion cofactor.
History
EC 4.2.3.16 created 2000 as EC 4.1.99.10, transferred 2000 to EC 4.2.3.16, modified 2003
Pathway
Monoterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K15088  
(4S)-limonene synthase
K21925  
(-)-camphene/tricyclene/(4S)-limonene/myrcene synthase
K21926  
(+)-alpha-terpineol/(4S)-limonene synthase
Genes
EGR: 
RCU: 
JCU: 
SLY: 
101245212(TPS3)
SPEN: 
SOT: 
Taxonomy
Reference
1  [PMID:9268308]
  Authors
Bohlmann J, Steele CL, Croteau R.
  Title
Monoterpene synthases from grand fir (Abies grandis). cDNA isolation, characterization, and functional expression of myrcene synthase, (-)-(4S)-limonene synthase, and (-)-(1S,5S)-pinene synthase.
  Journal
J. Biol. Chem. 272 (1997) 21784-92.
  Sequence
Reference
2  [PMID:8226816]
  Authors
Colby SM, Alonso WR, Katahira EJ, McGarvey DJ, Croteau R.
  Title
4S-limonene synthase from the oil glands of spearmint (Mentha spicata). cDNA isolation, characterization, and bacterial expression of the catalytically active monoterpene cyclase.
  Journal
J. Biol. Chem. 268 (1993) 23016-24.
  Sequence
Reference
3  [PMID:8806736]
  Authors
Yuba A, Yazaki K, Tabata M, Honda G, Croteau R.
  Title
cDNA cloning, characterization, and functional expression of 4S-(-)-limonene synthase from Perilla frutescens.
  Journal
Arch. Biochem. Biophys. 332 (1996) 280-7.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
110639-20-8
KEGG   REACTION: R02013Help
Entry
R02013                      Reaction                               

Name
geranyl-diphosphate diphosphate-lyase [cyclizing; (4S)-limonene-forming]
Definition
Geranyl diphosphate <=> (-)-Limonene + Diphosphate
Equation
Comment
(-)-(4S)-limonene synthase
Reaction class
C00341_C00521
Enzyme
Pathway
Monoterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K15088  
(4S)-limonene synthase [EC:4.2.3.16]
K21925  
(-)-camphene/tricyclene/(4S)-limonene/myrcene synthase [EC:4.2.3.117 4.2.3.105 4.2.3.16 4.2.3.15]
K21926  
(+)-alpha-terpineol/(4S)-limonene synthase [EC:4.2.3.112 4.2.3.16]
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