KEGG   ORTHOLOGY: K15766Help
Entry
K15766                      KO                                     

Name
absAa
Definition
2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha [EC:1.14.12.14]
Pathway
Benzoate degradation
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K15766  absAa; 2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
   00623 Toluene degradation
    K15766  absAa; 2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.14  2-aminobenzenesulfonate 2,3-dioxygenase
     K15766  absAa; 2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
BRITE hierarchy
Other DBs
Genes
AG: 
AAF14227(absAa)
TaxonomyKoalaUniProt
Reference
  Authors
Mampel J, Ruff J, Junker F, Cook AM.
  Title
The oxygenase component of the 2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1.
  Journal
Microbiology 145 ( Pt 11):3255-64 (1999)
DOI:10.1099/00221287-145-11-3255
  Sequence
Reference
PMID:8075807
  Authors
Junker F, Leisinger T, Cook AM
  Title
3-Sulphocatechol 2,3-dioxygenase and other dioxygenases (EC 1.13.11.2 and EC 1.14.12.-) in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. strain O-1.
  Journal
Microbiology 140 ( Pt 7):1713-22 (1994)
DOI:10.1099/13500872-140-7-1713
  Sequence

KEGG   ORTHOLOGY: K15767Help
Entry
K15767                      KO                                     

Name
absAb
Definition
2-aminobenzenesulfonate 2,3-dioxygenase subunit beta [EC:1.14.12.14]
Pathway
Benzoate degradation
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Xenobiotics biodegradation and metabolism
   00362 Benzoate degradation
    K15767  absAb; 2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
   00623 Toluene degradation
    K15767  absAb; 2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.12  With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
    1.14.12.14  2-aminobenzenesulfonate 2,3-dioxygenase
     K15767  absAb; 2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
BRITE hierarchy
Other DBs
Genes
AG: 
AAF14228(absAb)
TaxonomyKoalaUniProt
Reference
  Authors
Mampel J, Ruff J, Junker F, Cook AM.
  Title
The oxygenase component of the 2-aminobenzenesulfonate dioxygenase system from Alcaligenes sp. strain O-1.
  Journal
Microbiology 145 ( Pt 11):3255-64 (1999)
DOI:10.1099/00221287-145-11-3255
  Sequence
Reference
PMID:8075807
  Authors
Junker F, Leisinger T, Cook AM
  Title
3-Sulphocatechol 2,3-dioxygenase and other dioxygenases (EC 1.13.11.2 and EC 1.14.12.-) in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. strain O-1.
  Journal
Microbiology 140 ( Pt 7):1713-22 (1994)
DOI:10.1099/13500872-140-7-1713
  Sequence

KEGG   ENZYME: 1.14.12.14Help
Entry
EC 1.14.12.14               Enzyme                                 

Name
2-aminobenzenesulfonate 2,3-dioxygenase;
2-aminosulfobenzene 2,3-dioxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
BRITE hierarchy
Sysname
2-aminobenzenesulfonate,NADH:oxygen oxidoreductase (2,3-hydroxylating, ammonia-forming)
Reaction(IUBMB)
2-aminobenzenesulfonate + NADH + H+ + O2 = 2,3-dihydroxybenzenesulfonate + NH3 + NAD+ [RN:R05156]
Reaction(KEGG)
R05156;
(other) R05294
Show
Substrate
2-aminobenzenesulfonate [CPD:C06333];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
2,3-dihydroxybenzenesulfonate [CPD:C06336];
NH3 [CPD:C00014];
NAD+ [CPD:C00003]
History
EC 1.14.12.14 created 1999
Pathway
Benzoate degradation
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K15766  
2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha
K15767  
2-aminobenzenesulfonate 2,3-dioxygenase subunit beta
Reference
1  [PMID:8002948]
  Authors
Junker F, Field JA, Bangerter F, Ramsteiner K, Kohler HP, Joannou CL, Mason JR, Leisinger T, Cook AM.
  Title
Oxygenation and spontaneous deamination of 2-aminobenzenesulphonic acid in Alcaligenes sp. strain O-1 with subsequent meta ring cleavage and spontaneous desulphonation to 2-hydroxymuconic acid.
  Journal
Biochem. J. 300 ( Pt 2) (1994) 429-36.
Reference
2  [PMID:8075807]
  Authors
Junker F, Leisinger T, Cook AM.
  Title
3-Sulphocatechol 2,3-dioxygenase and other dioxygenases (EC 1.13.11.2 and EC 1.14.12.-) in the degradative pathways of 2-aminobenzenesulphonic, benzenesulphonic and 4-toluenesulphonic acids in Alcaligenes sp. strain O-1.
  Journal
Microbiology. 140 ( Pt 7) (1994) 1713-22.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
156621-16-8

KEGG   REACTION: R05294Help
Entry
R05294                      Reaction                               

Name
toluene-4-sulfonate,NADH:oxygen oxidoreductase (3,4-hydroxylating, sulfite-forming)
Definition
Toluene-4-sulfonate + Oxygen + NADH + H+ <=> 4-Methylcatechol + Sulfite + NAD+
Equation
Reaction class
C00003_C00004
C06677_C06730
Enzyme
Pathway
Toluene degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K15766  
2-aminobenzenesulfonate 2,3-dioxygenase subunit alpha [EC:1.14.12.14]
K15767  
2-aminobenzenesulfonate 2,3-dioxygenase subunit beta [EC:1.14.12.14]

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