KEGG   ORTHOLOGY: K15800Help
Entry
K15800                      KO                                     

Name
GAO1
Definition
germacrene A oxidase [EC:1.1.1.314 1.14.13.123]
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00909 Sesquiterpenoid and triterpenoid biosynthesis
    K15800  GAO1; germacrene A oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.1  Acting on the CH-OH group of donors
   1.1.1  With NAD+ or NADP+ as acceptor
    1.1.1.314  germacrene A alcohol dehydrogenase
     K15800  GAO1; germacrene A oxidase
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.123  germacrene A hydroxylase
     K15800  GAO1; germacrene A oxidase
BRITE hierarchy
Other DBs
Genes
AG: 
ADF32078(GAO1) ADF43080(CiGAO)
TaxonomyKoalaUniProt
Reference
  Authors
Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK
  Title
Biochemical conservation and evolution of germacrene A oxidase in asteraceae.
  Journal
J Biol Chem 285:16588-98 (2010)
DOI:10.1074/jbc.M110.111757
  Sequence

KEGG   ENZYME: 1.14.13.123Help
Entry
EC 1.14.13.123              Enzyme                                 

Name
germacrene A hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
(+)-germacrene-A,NADPH:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
(+)-germacrene A + NADPH + H+ + O2 = germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O [RN:R09549]
Reaction(KEGG)
Substrate
(+)-germacrene A [CPD:C16141];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
germacra-1(10),4,11(13)-trien-12-ol [CPD:C19676];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). This is probably part of the biosynthesis of many sesquiterpenoid lactones. In Lactuca sativa EC 1.14.13.123 is a mutifunctional enzyme with EC 1.1.1.314, germacrene A alcohol dehydrogenase [2].
History
EC 1.14.13.123 created 2011
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K15800  
germacrene A oxidase
Reference
1  [PMID:11299372]
  Authors
de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ
  Title
Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid  dehydrogenase(s) involved in sesquiterpene lactone biosynthesis.
  Journal
Plant. Physiol. 125 (2001) 1930-40.
  Sequence
Reference
2  [PMID:20351109]
  Authors
Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK
  Title
Biochemical conservation and evolution of germacrene A oxidase in asteraceae.
  Journal
J. Biol. Chem. 285 (2010) 16588-98.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09549Help
Entry
R09549                      Reaction                               

Name
(+)-germacrene-A,NADPH:oxygen oxidoreductase (12-hydroxylating)
Definition
(+)-Germacrene A + NADPH + H+ + Oxygen <=> Germacra-1(10),4,11(13)-trien-12-ol + NADP+ + H2O
Equation
Reaction class
C00005_C00006
C16141_C19676
Enzyme
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K15800  
germacrene A oxidase [EC:1.1.1.314 1.14.13.123]
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