KEGG   ORTHOLOGY: K15814Help
Entry
K15814                      KO                                     

Name
CYP93E1
Definition
beta-amyrin 24-hydroxylase [EC:1.14.99.43]
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00909 Sesquiterpenoid and triterpenoid biosynthesis
    K15814  CYP93E1; beta-amyrin 24-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.99  Miscellaneous
    1.14.99.43  beta-amyrin 24-hydroxylase
     K15814  CYP93E1; beta-amyrin 24-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP93 family
   K15814  CYP93E1; beta-amyrin 24-hydroxylase
BRITE hierarchy
Other DBs
RN: 
Genes
GMX: 
100037459(CYP93E1)
VRA: 
CAM: 
TaxonomyKoalaUniProt
Reference
  Authors
Steele CL, Gijzen M, Qutob D, Dixon RA
  Title
Molecular characterization of the enzyme catalyzing the aryl migration reaction of isoflavonoid biosynthesis in soybean.
  Journal
Arch Biochem Biophys 367:146-50 (1999)
DOI:10.1006/abbi.1999.1238
  Sequence
[gmx:100037459]
KEGG   ENZYME: 1.14.99.43Help
Entry
EC 1.14.99.43               Enzyme                                 

Name
beta-amyrin 24-hydroxylase;
sophoradiol 24-hydroxylase;
CYP93E1
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
BRITE hierarchy
Sysname
beta-amyrin,AH2:oxygen oxidoreductase (24-hydroxylating)
Reaction(IUBMB)
(1) beta-amyrin + reduced acceptor + O2 = 24-hydroxy-beta-amyrin + acceptor + H2O [RN:R09604];
(2) sophoradiol + reduced acceptor + O2 = 24-hydroxysophoradiol + acceptor + H2O [RN:R09605]
Reaction(KEGG)
Substrate
beta-amyrin [CPD:C08616];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007];
sophoradiol [CPD:C19733]
Product
24-hydroxy-beta-amyrin [CPD:C19732];
acceptor [CPD:C00028];
H2O [CPD:C00001];
24-hydroxysophoradiol [CPD:C08980]
Comment
A heme-thiolate protein (P-450).
History
EC 1.14.99.43 created 2011
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K15814  
beta-amyrin 24-hydroxylase
Genes
GMX: 
100037459(CYP93E1)
VRA: 
CAM: 
Taxonomy
Reference
1  [PMID:16478469]
  Authors
Shibuya M, Hoshino M, Katsube Y, Hayashi H, Kushiro T, Ebizuka Y
  Title
Identification of beta-amyrin and sophoradiol 24-hydroxylase by expressed sequence tag mining and functional expression assay.
  Journal
FEBS. J. 273 (2006) 948-59.
  Sequence
[gmx:100037459]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09604Help
Entry
R09604                      Reaction                               

Name
beta-amyrin,AH2:oxygen oxidoreductase (24-hydroxylating)
Definition
beta-Amyrin + Reduced acceptor + Oxygen <=> 24-Hydroxy-beta-amyrin + Acceptor + H2O
Equation
Comment
CYP93E1
Reaction class
C08616_C19732
Enzyme
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K15814  
beta-amyrin 24-hydroxylase [EC:1.14.99.43]
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