KEGG   ORTHOLOGY: K16047Help
Entry
K16047                      KO                                     

Name
hsaA
Definition
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase [EC:1.14.14.12]
Pathway
Steroid degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Xenobiotics biodegradation and metabolism
   00984 Steroid degradation
    K16047  hsaA; 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.14  With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.14.12  3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
     K16047  hsaA; 3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
BRITE hierarchy
Other DBs
RN: 
COG: 
Genes
XDO: 
XHO: 
PRE: 
PCQ: 
PcP3B5_25640(hsaA_1)
PPUT: 
PPUN: 
PFE: 
PVR: 
PPUU: 
PKC: 
PFZ: 
AV641_10560
PPSY: 
AOC04_07860
AVN: 
Avin_15060
AVL: 
AvCA_15060
AVD: 
AvCA6_15060
ACX: 
Achr_27810
SPL: 
Spea_2129
SHL: 
Shal_2101
COLW: 
A3Q33_18815
PHA: 
PSHAa0877
PTN: 
PTRA_a1004(hsaA)
ZAL: 
AZF00_12075
HJA: 
BST95_09495 BST95_19090
GBI: 
PG2T_01495 PG2T_04965
GPB: 
HDN1F_27120
AQL: 
BXU06_17040
RIN: 
ACS15_4394 ACS15_4395
REU: 
Reut_A1542
REH: 
H16_B0638(h16_B0638)
CNC: 
CNE_2c05830(hsaA)
CTI: 
RALTA_B0487
CBW: 
RR42_s2345
CGD: 
CR3_0799(hsaA)
CCUP: 
BKK81_19905
BUR: 
Bcep18194_B1413
BCJ: 
BCAM1647
BCEN: 
DM39_6202
BCEO: 
I35_5507
BCT: 
GEM_4008
BCED: 
DM42_6311
BCEP: 
APZ15_30820
BPYR: 
ABD05_27635
BCON: 
NL30_03950
BDF: 
WI26_21370
BTEI: 
WS51_05860
BPSL: 
WS57_05845
BMEC: 
WJ16_26975
BUG: 
BC1001_4364
BGE: 
BC1002_4695
BGO: 
BM43_6910
BUQ: 
AC233_10950
BPX: 
BUPH_00943
BFN: 
OI25_4616
PVE: 
UC34_06885
CTT: 
CtCNB1_1356
CTES: 
O987_07755
RBU: 
PG1C_04445
DAR: 
Daro_0902
AZI: 
AzCIB_1460
THU: 
AC731_008075
SUR: 
STAUR_5266
AVI: 
Avi_7417
BJA: 
bll6096
BJU: 
BJ6T_36130
BJP: 
RN69_17515
BRS: 
S23_21790
BRC: 
BCCGELA001_25895
BRAD: 
BF49_5537
BIC: 
LMTR13_10345
NWI: 
Nwi_0937
NHA: 
Nham_3406
BVV: 
BHK69_00515
XAU: 
Xaut_0933
RHZ: 
RHPLAN_33320 RHPLAN_36260
CSE: 
Cseg_1728
NAR: 
Saro_3648
NPP: 
PP1Y_Mpl3084
SPHK: 
SKP52_14060
SWI: 
Swit_3337
SPHM: 
G432_01990
STAX: 
MC45_16520
SPHI: 
TS85_13285
SSAN: 
NX02_12720
SSY: 
SLG_18380
SBD: 
ATN00_00945
SPHB: 
EP837_03391(hsaA)
BSM: 
BSM4216_1779
PNP: 
IJ22_48520
MTU: 
Rv3570c(hsaA)
MTV: 
RVBD_3570c
MTC: 
MT3675
MRA: 
MRA_3609
MTF: 
TBFG_13603
MTB: 
TBMG_03609
MTK: 
TBSG_03636
MTZ: 
TBXG_003584
MTG: 
MRGA327_22040
MTI: 
MRGA423_22560
MTE: 
CCDC5079_3311
MTUR: 
CFBS_3787
MTL: 
CCDC5180_3263
MTO: 
MTCTRI2_3635
MTD: 
UDA_3570c
MTN: 
ERDMAN_3917
MTJ: 
J112_19225
MTUB: 
MT7199_3633
MTUC: 
J113_25000
MTUE: 
J114_19095
MTX: 
M943_18360
MTUH: 
I917_25085
MTUL: 
TBHG_03511
MTUT: 
HKBT1_3772
MTUU: 
HKBT2_3781
MTQ: 
HKBS1_3784
MBO: 
Mb3601c
MBB: 
BCG_3635c
MBT: 
JTY_3636
MBM: 
BCGMEX_3633c
MBK: 
K60_037080
MBX: 
BCGT_3437
MBZ: 
LH58_19335
MAF: 
MAF_35830
MCE: 
MCAN_35831
MCQ: 
BN44_110065(hsaA)
MCV: 
BN43_90071(hsaA)
MCX: 
BN42_90068(hsaA)
MCZ: 
BN45_100084(hsaA)
MMIC: 
RN08_3947
MPA: 
MAP_0497
MAO: 
MAP4_3370
MAVI: 
RC58_16715
MAVU: 
RE97_16750
MAV: 
MAV_0591
MAVD: 
NF84_02655
MAVR: 
LA63_02720
MAVA: 
LA64_02730
MIT: 
OCO_05000
MIA: 
OCU_05040
MIE: 
LG41_02515
MIR: 
OCQ_05150
MID: 
MIP_00940
MYO: 
OEM_05060
MSM: 
MSMEG_6038
MSG: 
MSMEI_5877
MSB: 
LJ00_29850
MSN: 
LI99_29855
MSH: 
LI98_29860
MSA: 
Mycsm_05840
MUL: 
MUL_4141
MVA: 
Mvan_5308
MGI: 
Mflv_1463
MSP: 
Mspyr1_47040
MAB: 
MAB_0584
MABB: 
MASS_0553
MMV: 
MYCMA_0297
MAY: 
LA62_02955
MABO: 
NF82_02950
MABL: 
MMASJCM_0573
MAZ: 
LA61_02870
MAK: 
LH56_19695
MYS: 
NF92_20205
MYC: 
NF90_20210
MCHE: 
BB28_02785
MIZ: 
BAB75_03535
MMC: 
Mmcs_4709
MKM: 
Mkms_4795
MJL: 
Mjls_5094
MJD: 
JDM601_3854
MMI: 
MMAR_5065
MMAE: 
MMARE11_48800
MRH: 
MycrhN_2243
MMM: 
W7S_02440
MCB: 
Mycch_4675
MLI: 
MULP_05323
MKN: 
MKAN_12130
MKS: 
LG40_11975
MKI: 
LH54_12120
MNE: 
D174_24710
MYV: 
G155_08435 G155_12940 G155_12945 G155_24370 G155_26485
MYE: 
AB431_26555
MGO: 
AFA91_10580
MFT: 
XA26_16880 XA26_26610 XA26_49320 XA26_53630
MHAD: 
B586_04445
MPHL: 
MPHLCCUG_00491(hsaA_1) MPHLCCUG_03656(hsaA_5) MPHLCCUG_03657(hsaA_6)
MVQ: 
MYVA_3715 MYVA_5218
ASD: 
AS9A_0924
NFA: 
NFA_33590 NFA_4740
NFR: 
ERS450000_00305(hsaA_1) ERS450000_00694(hsaA_3) ERS450000_03762(hsaA_8)
NCY: 
NOCYR_0503
NBR: 
O3I_002675
NNO: 
NONO_c05010(hsaA) NONO_c58890
NSL: 
BOX37_02185
NSR: 
NS506_01262(hsaA) NS506_04326 NS506_04702 NS506_06809
RHA: 
RHA1_ro04539(hsaA)
RER: 
RER_07550 RER_51120
REY: 
O5Y_03670 O5Y_24230
REB: 
XU06_03910 XU06_24650
ROP: 
ROP_44540
ROA: 
Pd630_LPD00991 Pd630_LPD02436
REQ: 
REQ_01440 REQ_06800 REQ_08840 REQ_43720
RPY: 
Y013_19025
RAV: 
AAT18_03965
RHW: 
BFN03_15200
GBR: 
Gbro_0920
GPO: 
GPOL_c41090(hsaA)
GOR: 
KTR9_0820(hsaA)
GOQ: 
ACH46_03240
GTA: 
BCM27_05110
TPR: 
Tpau_3845
DTM: 
BJL86_0698
SCB: 
SCAB_14821
SALU: 
DC74_7445
SALL: 
SAZ_38430
SVT: 
SVTN_35900 SVTN_35905
STRM: 
M444_37895
SLS: 
SLINC_0828
CPHY: 
B5808_02315
NCA: 
Noca_2776
NDK: 
I601_3470(hsaA_2)
PSIM: 
KR76_14480
TCU: 
Tcur_3508
SRO: 
Sros_6977
AMD: 
AMED_2522
AMN: 
RAM_12820
AMM: 
AMES_2494
AMZ: 
B737_2495
AOI: 
AORI_2484
AJA: 
AJAP_26405(hsaA)
AMQ: 
AMETH_4838
ALU: 
BB31_28890
KAL: 
KALB_5124
KPHY: 
AOZ06_29015 AOZ06_31145 AOZ06_42790
LED: 
BBK82_38925 BBK82_44665
ALL: 
CRK57806 CRK59457 CRK59594
STP: 
Strop_2671
SAQ: 
Sare_2879
AMS: 
AMIS_19180
TBI: 
Tbis_1633
 » show all
TaxonomyKoalaUniProt
Reference
  Authors
Dresen C, Lin LY, D'Angelo I, Tocheva EI, Strynadka N, Eltis LD
  Title
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.
  Journal
J Biol Chem 285:22264-75 (2010)
DOI:10.1074/jbc.M109.099028
  Sequence
[mtu:Rv3570c]
Reference
  Authors
Ouellet H, Johnston JB, de Montellano PR
  Title
Cholesterol catabolism as a therapeutic target in Mycobacterium tuberculosis.
  Journal
Trends Microbiol 19:530-9 (2011)
DOI:10.1016/j.tim.2011.07.009
KEGG   ENZYME: 1.14.14.12Help
Entry
EC 1.14.14.12               Enzyme                                 

Name
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase;
HsaA
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione,FMNH2:oxygen oxidoreductase
Reaction(IUBMB)
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMNH2 + O2 = 3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O [RN:R09819]
Reaction(KEGG)
Substrate
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C19944];
FMNH2 [CPD:C01847];
O2 [CPD:C00007]
Product
3,4-dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione [CPD:C04793];
FMN [CPD:C00061];
H2O [CPD:C00001]
Comment
This bacterial enzyme participates in the degradation of several steroids, including cholesterol and testosterone. It can use either FADH or FMNH2 as flavin cofactor. The enzyme forms a two-component system with a reductase (HsaB) that utilizes NADH to reduce the flavin, which is then transferred to the oxygenase subunit.
History
EC 1.14.14.12 created 2011
Pathway
Steroid degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K16047  
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase
Genes
XDO: 
XHO: 
PRE: 
PCQ: 
PcP3B5_25640(hsaA_1)
PPUT: 
PPUN: 
PFE: 
PVR: 
PPUU: 
PKC: 
PFZ: 
AV641_10560
PPSY: 
AOC04_07860
AVN: 
Avin_15060
AVL: 
AvCA_15060
AVD: 
AvCA6_15060
ACX: 
Achr_27810
SPL: 
Spea_2129
SHL: 
Shal_2101
COLW: 
A3Q33_18815
PHA: 
PSHAa0877
PTN: 
PTRA_a1004(hsaA)
ZAL: 
AZF00_12075
HJA: 
BST95_09495 BST95_19090
GBI: 
PG2T_01495 PG2T_04965
GPB: 
HDN1F_27120
AQL: 
BXU06_17040
RIN: 
ACS15_4394 ACS15_4395
REU: 
Reut_A1542
REH: 
H16_B0638(h16_B0638)
CNC: 
CNE_2c05830(hsaA)
CTI: 
RALTA_B0487
CBW: 
RR42_s2345
CGD: 
CR3_0799(hsaA)
CCUP: 
BKK81_19905
BUR: 
Bcep18194_B1413
BCJ: 
BCAM1647
BCEN: 
DM39_6202
BCEO: 
I35_5507
BCT: 
GEM_4008
BCED: 
DM42_6311
BCEP: 
APZ15_30820
BPYR: 
ABD05_27635
BCON: 
NL30_03950
BDF: 
WI26_21370
BTEI: 
WS51_05860
BPSL: 
WS57_05845
BMEC: 
WJ16_26975
BUG: 
BC1001_4364
BGE: 
BC1002_4695
BGO: 
BM43_6910
BUQ: 
AC233_10950
BPX: 
BUPH_00943
BFN: 
OI25_4616
PVE: 
UC34_06885
CTT: 
CtCNB1_1356
CTES: 
O987_07755
RBU: 
PG1C_04445
DAR: 
Daro_0902
AZI: 
AzCIB_1460
THU: 
AC731_008075
SUR: 
STAUR_5266
AVI: 
Avi_7417
BJA: 
bll6096
BJU: 
BJ6T_36130
BJP: 
RN69_17515
BRS: 
S23_21790
BRC: 
BCCGELA001_25895
BRAD: 
BF49_5537
BIC: 
LMTR13_10345
NWI: 
Nwi_0937
NHA: 
Nham_3406
BVV: 
BHK69_00515
XAU: 
Xaut_0933
RHZ: 
RHPLAN_33320 RHPLAN_36260
CSE: 
Cseg_1728
NAR: 
Saro_3648
NPP: 
PP1Y_Mpl3084
SPHK: 
SKP52_14060
SWI: 
Swit_3337
SPHM: 
G432_01990
STAX: 
MC45_16520
SPHI: 
TS85_13285
SSAN: 
NX02_12720
SSY: 
SLG_18380
SBD: 
ATN00_00945
SPHB: 
EP837_03391(hsaA)
BSM: 
BSM4216_1779
PNP: 
IJ22_48520
MTU: 
Rv3570c(hsaA)
MTV: 
RVBD_3570c
MTC: 
MT3675
MRA: 
MRA_3609
MTF: 
TBFG_13603
MTB: 
TBMG_03609
MTK: 
TBSG_03636
MTZ: 
TBXG_003584
MTG: 
MRGA327_22040
MTI: 
MRGA423_22560
MTE: 
CCDC5079_3311
MTUR: 
CFBS_3787
MTL: 
CCDC5180_3263
MTO: 
MTCTRI2_3635
MTD: 
UDA_3570c
MTN: 
ERDMAN_3917
MTJ: 
J112_19225
MTUB: 
MT7199_3633
MTUC: 
J113_25000
MTUE: 
J114_19095
MTX: 
M943_18360
MTUH: 
I917_25085
MTUL: 
TBHG_03511
MTUT: 
HKBT1_3772
MTUU: 
HKBT2_3781
MTQ: 
HKBS1_3784
MBO: 
Mb3601c
MBB: 
BCG_3635c
MBT: 
JTY_3636
MBM: 
BCGMEX_3633c
MBK: 
K60_037080
MBX: 
BCGT_3437
MBZ: 
LH58_19335
MAF: 
MAF_35830
MCE: 
MCAN_35831
MCQ: 
BN44_110065(hsaA)
MCV: 
BN43_90071(hsaA)
MCX: 
BN42_90068(hsaA)
MCZ: 
BN45_100084(hsaA)
MMIC: 
RN08_3947
MPA: 
MAP_0497
MAO: 
MAP4_3370
MAVI: 
RC58_16715
MAVU: 
RE97_16750
MAV: 
MAV_0591
MAVD: 
NF84_02655
MAVR: 
LA63_02720
MAVA: 
LA64_02730
MIT: 
OCO_05000
MIA: 
OCU_05040
MIE: 
LG41_02515
MIR: 
OCQ_05150
MID: 
MIP_00940
MYO: 
OEM_05060
MSM: 
MSMEG_6038
MSG: 
MSMEI_5877
MSB: 
LJ00_29850
MSN: 
LI99_29855
MSH: 
LI98_29860
MSA: 
Mycsm_05840
MUL: 
MUL_4141
MVA: 
Mvan_5308
MGI: 
Mflv_1463
MSP: 
Mspyr1_47040
MAB: 
MAB_0584
MABB: 
MASS_0553
MMV: 
MYCMA_0297
MAY: 
LA62_02955
MABO: 
NF82_02950
MABL: 
MMASJCM_0573
MAZ: 
LA61_02870
MAK: 
LH56_19695
MYS: 
NF92_20205
MYC: 
NF90_20210
MCHE: 
BB28_02785
MIZ: 
BAB75_03535
MMC: 
Mmcs_4709
MKM: 
Mkms_4795
MJL: 
Mjls_5094
MJD: 
JDM601_3854
MMI: 
MMAR_5065
MMAE: 
MMARE11_48800
MRH: 
MycrhN_2243
MMM: 
W7S_02440
MCB: 
Mycch_4675
MLI: 
MULP_05323
MKN: 
MKAN_12130
MKS: 
LG40_11975
MKI: 
LH54_12120
MNE: 
D174_24710
MYV: 
G155_08435 G155_12940 G155_12945 G155_24370 G155_26485
MYE: 
AB431_26555
MGO: 
AFA91_10580
MFT: 
XA26_16880 XA26_26610 XA26_49320 XA26_53630
MHAD: 
B586_04445
MPHL: 
MPHLCCUG_00491(hsaA_1) MPHLCCUG_03656(hsaA_5) MPHLCCUG_03657(hsaA_6)
MVQ: 
MYVA_3715 MYVA_5218
ASD: 
AS9A_0924
NFA: 
NFA_33590 NFA_4740
NFR: 
ERS450000_00305(hsaA_1) ERS450000_00694(hsaA_3) ERS450000_03762(hsaA_8)
NCY: 
NOCYR_0503
NBR: 
O3I_002675
NNO: 
NONO_c05010(hsaA) NONO_c58890
NSL: 
BOX37_02185
NSR: 
NS506_01262(hsaA) NS506_04326 NS506_04702 NS506_06809
RHA: 
RHA1_ro04539(hsaA)
RER: 
RER_07550 RER_51120
REY: 
O5Y_03670 O5Y_24230
REB: 
XU06_03910 XU06_24650
ROP: 
ROP_44540
ROA: 
Pd630_LPD00991 Pd630_LPD02436
REQ: 
REQ_01440 REQ_06800 REQ_08840 REQ_43720
RPY: 
Y013_19025
RAV: 
AAT18_03965
RHW: 
BFN03_15200
GBR: 
Gbro_0920
GPO: 
GPOL_c41090(hsaA)
GOR: 
KTR9_0820(hsaA)
GOQ: 
ACH46_03240
GTA: 
BCM27_05110
TPR: 
Tpau_3845
DTM: 
BJL86_0698
SCB: 
SCAB_14821
SALU: 
DC74_7445
SALL: 
SAZ_38430
SVT: 
SVTN_35900 SVTN_35905
STRM: 
M444_37895
SLS: 
SLINC_0828
CPHY: 
B5808_02315
NCA: 
Noca_2776
NDK: 
I601_3470(hsaA_2)
PSIM: 
KR76_14480
TCU: 
Tcur_3508
SRO: 
Sros_6977
AMD: 
AMED_2522
AMN: 
RAM_12820
AMM: 
AMES_2494
AMZ: 
B737_2495
AOI: 
AORI_2484
AJA: 
AJAP_26405(hsaA)
AMQ: 
AMETH_4838
ALU: 
BB31_28890
KAL: 
KALB_5124
KPHY: 
AOZ06_29015 AOZ06_31145 AOZ06_42790
LED: 
BBK82_38925 BBK82_44665
ALL: 
CRK57806 CRK59457 CRK59594
STP: 
Strop_2671
SAQ: 
Sare_2879
AMS: 
AMIS_19180
TBI: 
Tbis_1633
 » show all
Taxonomy
Reference
1  [PMID:20448045]
  Authors
Dresen C, Lin LY, D'Angelo I, Tocheva EI, Strynadka N, Eltis LD
  Title
A flavin-dependent monooxygenase from Mycobacterium tuberculosis involved in cholesterol catabolism.
  Journal
J. Biol. Chem. 285 (2010) 22264-75.
  Sequence
[mtu:Rv3570c]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09819Help
Entry
R09819                      Reaction                               

Name
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione,FMNH2:oxygen oxidoreductase
Definition
3-Hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + Reduced FMN + Oxygen <=> 3,4-Dihydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione + FMN + H2O
Equation
Comment
FMN is re-reduced by NADH (see R05705)
Reaction class
C00061_C01847
C04793_C19944
Enzyme
Pathway
Steroid degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K16047  
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase [EC:1.14.14.12]
K16048  
3-hydroxy-9,10-secoandrosta-1,3,5(10)-triene-9,17-dione monooxygenase reductase component [EC:1.5.1.-]
DBGET integrated database retrieval system