KEGG   ORTHOLOGY: K16085Help
Entry
K16085                      KO                                     

Name
CYP99A3
Definition
9beta-pimara-7,15-diene oxidase [EC:1.14.13.144]
Pathway
Diterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K16085  CYP99A3; 9beta-pimara-7,15-diene oxidase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.144  9beta-pimara-7,15-diene oxidase
     K16085  CYP99A3; 9beta-pimara-7,15-diene oxidase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP99 family
   K16085  CYP99A3; 9beta-pimara-7,15-diene oxidase
BRITE hierarchy
Other DBs
Genes
OSA: 
4335091(CYP99A3)
DOSA: 
Os04t0178400-01(Os04g0178400)
TaxonomyKoalaUniProt
Reference
  Authors
Wang Q, Hillwig ML, Peters RJ
  Title
CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice.
  Journal
Plant J 65:87-95 (2011)
DOI:10.1111/j.1365-313X.2010.04408.x
  Sequence
[osa:4335091]
KEGG   ENZYME: 1.14.13.144Help
Entry
EC 1.14.13.144              Enzyme                                 

Name
9beta-pimara-7,15-diene oxidase;
CYP99A3
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase
Reaction(IUBMB)
9beta-pimara-7,15-diene + 3 O2 + 3 NADPH + 3 H+ = 9beta-pimara-7,15-dien-19-oate + 3 NADP+ + 4 H2O (overall reaction) [RN:R09865];
(1a) 9beta-pimara-7,15-diene + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-ol + NADP+ + H2O [RN:R09862];
(1b) 9beta-pimara-7,15-dien-19-ol + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-al + NADP+ + 2 H2O [RN:R09863];
(1c) 9beta-pimara-7,15-dien-19-al + O2 + NADPH + H+ = 9beta-pimara-7,15-dien-19-oate + NADP+ + H2O [RN:R09864]
Reaction(KEGG)
Substrate
9beta-pimara-7,15-diene [CPD:C18225];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Product
9beta-pimara-7,15-dien-19-oate [CPD:C20149];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
9beta-pimara-7,15-dien-19-ol [CPD:C20147];
9beta-pimara-7,15-dien-19-al [CPD:C20148]
Comment
Requires cytochrome P-450. A rice, Oryza sativa, enzyme involved in the phytoalexin momilactone biosynthesis. It also acts similarly on 9beta-stemod-13(17)-ene.
History
EC 1.14.13.144 created 2012
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K16085  
9beta-pimara-7,15-diene oxidase
Genes
OSA: 
4335091(CYP99A3)
DOSA: 
Os04t0178400-01(Os04g0178400)
Taxonomy
Reference
1  [PMID:21175892]
  Authors
Wang Q, Hillwig ML, Peters RJ
  Title
CYP99A3: functional identification of a diterpene oxidase from the momilactone biosynthetic gene cluster in rice.
  Journal
Plant. J. 65 (2011) 87-95.
  Sequence
[osa:4335091]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09865Help
Entry
R09865                      Reaction                               

Name
9beta-pimara-7,15-diene,NADPH:oxygen 19-oxidoreductase
Definition
9beta-Pimara-7,15-diene + 3 Oxygen + 3 NADPH + 3 H+ <=> 9beta-Pimara-7,15-dien-19-oate + 3 NADP+ + 4 H2O
Equation
C18225 + 3 C00007 + 3 C00005 + 3 C00080 <=> C20149 + 3 C00006 + 4 C00001
Comment
three-step reaction (see R09862+R09863+R09864)
Reaction class
C00005_C00006
C18225_C20149
Enzyme
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K16085  
9beta-pimara-7,15-diene oxidase [EC:1.14.13.144]
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