KEGG   ORTHOLOGY: K18076Help
Entry
K18076                      KO                                     

Name
tphB
Definition
1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase [EC:1.3.1.53]
Pathway
Polycyclic aromatic hydrocarbon degradation
Degradation of aromatic compounds
Module
M00624  
Terephthalate degradation, terephthalate => 3,4-dihydroxybenzoate
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Overview
   01220 Degradation of aromatic compounds
    K18076  tphB; 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
  Xenobiotics biodegradation and metabolism
   00624 Polycyclic aromatic hydrocarbon degradation
    K18076  tphB; 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
KEGG modules [BR:ko00002]
 Pathway module
  Secondary metabolism
   Aromatics degradation
    M00624  Terephthalate degradation, terephthalate => 3,4-dihydroxybenzoate
     K18076  tphB; 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.3  Acting on the CH-CH group of donors
   1.3.1  With NAD+ or NADP+ as acceptor
    1.3.1.53  (3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase
     K18076  tphB; 1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
BRITE hierarchy
Other DBs
RN: 
GO: 
Genes
VPD: 
CTT: 
CTES: 
RTA: 
TaxonomyKoalaUniProt
Reference
  Authors
Sasoh M, Masai E, Ishibashi S, Hara H, Kamimura N, Miyauchi K, Fukuda M
  Title
Characterization of the terephthalate degradation genes of Comamonas sp. strain E6.
  Journal
Appl Environ Microbiol 72:1825-32 (2006)
DOI:10.1128/AEM.72.3.1825-1832.2006
  Sequence
Reference
PMID:8565920
  Authors
Wang YZ, Zhou Y, Zylstra GJ
  Title
Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D.
  Journal
Environ Health Perspect 103 Suppl 5:9-12 (1995)
  Sequence

KEGG   ENZYME: 1.3.1.53Help
Entry
EC 1.3.1.53                 Enzyme                                 

Name
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase;
(1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase;
terephthalate 1,2-cis-dihydrodiol dehydrogenase;
cis-4,5-dihydroxycyclohexa-1(6),2-diene-1,4-dicarboxylate:NAD+ oxidoreductase (decarboxylating)
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate:NAD+ oxidoreductase
Reaction(IUBMB)
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate + NAD+ = 3,4-dihydroxybenzoate + CO2 + NADH [RN:R01633]
Reaction(KEGG)
Substrate
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate [CPD:C06318];
NAD+ [CPD:C00003]
Product
3,4-dihydroxybenzoate [CPD:C00230];
CO2 [CPD:C00011];
NADH [CPD:C00004]
Comment
Requires FeII. Involved in the terephthalate degradation pathway in bacteria [2].
History
EC 1.3.1.53 created 1999 (EC 1.3.1.61 created 2000, incorporated 2007)
Pathway
Polycyclic aromatic hydrocarbon degradation
Metabolic pathways
Microbial metabolism in diverse environments
Orthology
K18076  
1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase
Genes
VPD: 
CTT: 
CTES: 
RTA: 
Taxonomy
Reference
1
  Authors
Saller, E., Laue, H.R., Schlafli Oppenberg, H.R. and Cook, A.M.
  Title
Purification and some properties of (1R,2S)-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2.
  Journal
FEMS Microbiol. Lett. 130 (1996) 97-102.
Reference
2  [PMID:8565920]
  Authors
Wang YZ, Zhou Y, Zylstra GJ.
  Title
Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D.
  Journal
Environ. Health. Perspect. 103 Suppl 5 (1995) 9-12.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
162032-77-1
KEGG   REACTION: R01633Help
Entry
R01633                      Reaction                               

Name
(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate:NAD+ oxidoreductase
Definition
(3S,4R)-3,4-Dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate + NAD+ <=> 3,4-Dihydroxybenzoate + CO2 + NADH + H+
Equation
Reaction class
C00003_C00004
C00230_C06318
Enzyme
Pathway
Polycyclic aromatic hydrocarbon degradation
Metabolic pathways
Microbial metabolism in diverse environments
Degradation of aromatic compounds
Module
M00624  
Terephthalate degradation, terephthalate => 3,4-dihydroxybenzoate
Orthology
K18076  
1,2-dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylate dehydrogenase [EC:1.3.1.53]
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