KEGG   ORTHOLOGY: K20512Help
Entry
K20512                      KO                                     

Name
K20512
Definition
taxoid 14beta-hydroxylase [EC:1.14.13.146]
Pathway
Diterpenoid biosynthesis
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Metabolism of terpenoids and polyketides
   00904 Diterpenoid biosynthesis
    K20512  K20512; taxoid 14beta-hydroxylase
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.146  taxoid 14beta-hydroxylase
     K20512  K20512; taxoid 14beta-hydroxylase
Cytochrome P450 [BR:ko00199]
 Cytochrome P450, plant type
  CYP725 family
   K20512  K20512; taxoid 14beta-hydroxylase
BRITE hierarchy
Other DBs
RN: 
GO: 
Genes
AG: 
TaxonomyKoalaUniProt
Reference
  Authors
Jennewein S, Rithner CD, Williams RM, Croteau R
  Title
Taxoid metabolism: Taxoid 14beta-hydroxylase is a cytochrome P450-dependent monooxygenase.
  Journal
Arch Biochem Biophys 413:262-70 (2003)
DOI:10.1016/S0003-9861(03)00090-0
  Sequence

KEGG   ENZYME: 1.14.13.146Help
Entry
EC 1.14.13.146              Enzyme                                 

Name
taxoid 14beta-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl-acetate,NADPH:oxygen 14-oxidoreductase
Reaction(IUBMB)
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + O2 + NADPH + H+ = 10beta,14beta-dihydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O [RN:R09867]
Reaction(KEGG)
Substrate
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate [CPD:C11898];
O2 [CPD:C00007];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Product
10beta,14beta-dihydroxytaxa-4(20),11-dien-5alpha-yl acetate [CPD:C20151];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
Requires cytochrome P450. From the yew Taxus cuspidata. Also acts on taxa-4(20),11-dien-5alpha-yl acetate.
History
EC 1.14.13.146 created 2012
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K20512  
taxoid 14beta-hydroxylase
Reference
1  [PMID:12729625]
  Authors
Jennewein S, Rithner CD, Williams RM, Croteau R
  Title
Taxoid metabolism: Taxoid 14beta-hydroxylase is a cytochrome P450-dependent monooxygenase.
  Journal
Arch. Biochem. Biophys. 413 (2003) 262-70.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
KEGG   REACTION: R09867Help
Entry
R09867                      Reaction                               

Name
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl-acetate,NADPH:oxygen 14-oxidoreductase
Definition
Taxa-4(20),11(12)-dien-5alpha-acetoxy-10beta-ol + Oxygen + NADPH + H+ <=> 10beta,14beta-Dihydroxytaxa-4(20),11-dien-5alpha-yl acetate + NADP+ + H2O
Equation
Reaction class
C00005_C00006
C11898_C20151
Enzyme
Pathway
Diterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K20512  
taxoid 14beta-hydroxylase [EC:1.14.13.146]
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