ferruginol synthase [EC:1.14.13.190]

PMID:23812755

Guo J, Zhou YJ, Hillwig ML, Shen Y, Yang L, Wang Y, Zhang X, Liu W, Peters RJ, Chen X, Zhao ZK, Huang L

CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts.

Proc Natl Acad Sci U S A 110:12108-13 (2013)
DOI:10.1073/pnas.1218061110

[ag:AGN04215]

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor

miltiradiene,NADPH:oxygen oxidoreductase (ferruginol-forming)

abieta-8,11,13-triene + NADPH + H+ + O2 = ferruginol + NADP+ + H2O [RN:R10727]

R10727

abieta-8,11,13-triene [CPD:C21425];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]

The enzyme is found in some members of the Lamiaceae (mint family). The enzyme from Rosmarinus officinalis (rosemary) is involved in biosynthesis of carnosic acid, while the enzyme from the Chinese medicinal herb Salvia miltiorrhiza is involved in the biosynthesis of the tanshinones, abietane-type norditerpenoid naphthoquinones that are the main lipophilic bioactive components found in the plant.

EC 1.14.13.190 created 2014, modified 2015

1  [PMID:23812755]

Guo J, Zhou YJ, Hillwig ML, Shen Y, Yang L, Wang Y, Zhang X, Liu W, Peters RJ, Chen X, Zhao ZK, Huang L

CYP76AH1 catalyzes turnover of miltiradiene in tanshinones biosynthesis and enables heterologous production of ferruginol in yeasts.

Proc. Natl. Acad. Sci. U. S. A. 110 (2013) 12108-13.

[ag:AGN04215]

2  [PMID:24108414]

Zi J, Peters RJ

Characterization of CYP76AH4 clarifies phenolic diterpenoid biosynthesis in the Lamiaceae.

Org. Biomol. Chem. 11 (2013) 7650-2.

3  [PMID:26020634]

Bozic D, Papaefthimiou D, Bruckner K, de Vos RC, Tsoleridis CA, Katsarou D, Papanikolaou A, Pateraki I, Chatzopoulou FM, Dimitriadou E, Kostas S, Manzano D, Scheler U, Ferrer A, Tissier A, Makris AM, Kampranis SC, Kanellis AK

Towards Elucidating Carnosic Acid Biosynthesis in Lamiaceae: Functional Characterization of the Three First Steps of the Pathway in Salvia fruticosa and  Rosmarinus officinalis.

PLoS. One. 10 (2015) e0124106.