KEGG   ORTHOLOGY: K21726Help
Entry
K21726                      KO                                     

Name
npcA
Definition
4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, oxygenase component [EC:1.14.13.29 1.14.13.166]
Pathway
Aminobenzoate degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K21726  npcA; 4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, oxygenase component
Enzymes [BR:ko01000]
 1. Oxidoreductases
  1.14  Acting on paired donors, with incorporation or reduction of molecular oxygen
   1.14.13  With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
    1.14.13.29  4-nitrophenol 2-monooxygenase
     K21726  npcA; 4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, oxygenase component
    1.14.13.166  4-nitrocatechol 4-monooxygenase
     K21726  npcA; 4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, oxygenase component
BRITE hierarchy
Other DBs
Genes
ARY: 
ACH: 
AG: 
BAD30042(npcA)
TaxonomyKoalaUniProt
Reference
  Authors
Kitagawa W, Kimura N, Kamagata Y
  Title
A novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, Rhodococcus opacus SAO101.
  Journal
J Bacteriol 186:4894-902 (2004)
DOI:10.1128/JB.186.15.4894-4902.2004
  Sequence

KEGG   ORTHOLOGY: K21727Help
Entry
K21727                      KO                                     

Name
npcB
Definition
4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, reductase component
Pathway
Aminobenzoate degradation
Brite
KEGG Orthology (KO) [BR:ko00001]
 Metabolism
  Xenobiotics biodegradation and metabolism
   00627 Aminobenzoate degradation
    K21727  npcB; 4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, reductase component
BRITE hierarchy
Other DBs
RN: 
COG: 
Genes
SRC: 
MIH: 
ARY: 
ACH: 
RXY: 
CWO: 
AG: 
BAD30041(npcB)
TaxonomyKoalaUniProt
Reference
  Authors
Kitagawa W, Kimura N, Kamagata Y
  Title
A novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, Rhodococcus opacus SAO101.
  Journal
J Bacteriol 186:4894-902 (2004)
DOI:10.1128/JB.186.15.4894-4902.2004
  Sequence

KEGG   ENZYME: 1.14.13.29Help
Entry
EC 1.14.13.29               Enzyme                                 

Name
4-nitrophenol 2-monooxygenase;
4-nitrophenol hydroxylase;
4-nitrophenol-2-hydroxylase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor
BRITE hierarchy
Sysname
4-nitrophenol,NADH:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
4-nitrophenol + NADH + H+ + O2 = 4-nitrocatechol + NAD+ + H2O [RN:R03023]
Reaction(KEGG)
Substrate
4-nitrophenol [CPD:C00870];
NADH [CPD:C00004];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
4-nitrocatechol [CPD:C02235];
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Comment
A flavoprotein (FAD).
History
EC 1.14.13.29 created 1989
Pathway
Aminobenzoate degradation
Microbial metabolism in diverse environments
Orthology
K21726  
4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, oxygenase component
Genes
ARY: 
ACH: 
Taxonomy
Reference
1  [PMID:6477623]
  Authors
Mitra D, Vaidyanathan CS.
  Title
A new 4-nitrophenol 2-hydroxylase from a Nocardia sp.
  Journal
Biochem. Int. 8 (1984) 609-15.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
UM-BBD (Biocatalysis/Biodegradation Database): 
BRENDA, the Enzyme Database: 
CAS: 
91116-87-9
KEGG   REACTION: R03023Help
Entry
R03023                      Reaction                               

Name
4-nitrophenol,NADH:oxygen oxidoreductase (2-hydroxylating)
Definition
4-Nitrophenol + Oxygen + NADH + H+ <=> 4-Nitrocatechol + NAD+ + H2O
Equation
Reaction class
C00003_C00004
C00870_C02235
Enzyme
Pathway
Aminobenzoate degradation
Microbial metabolism in diverse environments
Orthology
K21726  
4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, oxygenase component [EC:1.14.13.29 1.14.13.166]
K21727  
4-nitrophenol 2-monooxygenase / 4-nitrocatechol 4-monooxygenase, reductase component
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