KEGG   PATHWAY: dme03030Help
Entry
dme03030                    Pathway                                

Name
DNA replication - Drosophila melanogaster (fruit fly)
Description
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Class
Genetic Information Processing; Replication and repair
BRITE hierarchy
Pathway map
DNA replication
dme03030

All organismsOrtholog table
Module
DNA polymerase alpha / primase complex [PATH:dme03030]
DNA polymerase epsilon complex [PATH:dme03030]
MCM complex [PATH:dme03030]
RPA complex [PATH:dme03030]
RF-C complex [PATH:dme03030]
Organism
Drosophila melanogaster (fruit fly) [GN:dme]
Gene
mtSSB; mitochondrial single stranded DNA-binding protein [KO:K03111]
rnh1; ribonuclease H1, isoform A [KO:K03469] [EC:3.1.26.4]
RpA-70; replication protein A 70 [KO:K07466]
PCNA; proliferating cell nuclear antigen, isoform A [KO:K04802]
CG10262; uncharacterized protein [KO:K04802]
CG2990, isoform C [KO:K10742] [EC:3.6.4.12]
Fen1; flap endonuclease 1 [KO:K04799] [EC:3.-.-.-]
DNA-ligI; DNA ligase I [KO:K10747] [EC:6.5.1.7 6.5.1.6 6.5.1.1]
DNApol-alpha180; DNA polymerase alpha 180kD [KO:K02320] [EC:2.7.7.7]
DNApol-alpha73; DNA polymerase alpha 73kD [KO:K02321]
DNApol-alpha50; DNA polymerase alpha 50kD [KO:K02684] [EC:2.7.7.-]
DNApol-alpha60; DNA polymerase alpha 60kD [KO:K02685] [EC:2.7.7.-]
DNApol-delta; DNA-polymerase-delta [KO:K02327] [EC:2.7.7.7]
CG12018; uncharacterized protein [KO:K02328]
Pol32; Pol32 polymerase delta subunit, isoform B [KO:K03504]
DNApol-epsilon255; DNA polymerase epsilon 255kD subunit [KO:K02324] [EC:2.7.7.7]
DNApol-epsilon58; DNA polymerase epsilon 58kD subunit [KO:K02325] [EC:2.7.7.7]
Chrac-14; chromatin accessibility complex 14kD protein [KO:K02326] [EC:2.7.7.7]
Mes4; Mesoderm-expressed 4, isoform B [KO:K03506] [EC:2.7.7.7]
Mcm2; minichromosome maintenance 2 [KO:K02540] [EC:3.6.4.12]
Mcm3; minichromosome maintenance 3, isoform A [KO:K02541] [EC:3.6.4.12]
dpa; disc proliferation abnormal, isoform B [KO:K02212] [EC:3.6.4.12]
Mcm5; minichromosome maintenance 5 [KO:K02209] [EC:3.6.4.12]
Mcm6; minichromosome maintenance 6 [KO:K02542] [EC:3.6.4.12]
Mcm7; minichromosome maintenance 7 [KO:K02210] [EC:3.6.4.12]
RPA2; replication protein A2, isoform B [KO:K10739]
RPA3; replication protein A3, isoform B [KO:K10740]
Gnf1; germ line transcription factor 1, isoform A [KO:K10754]
CG8142 [KO:K10755]
RfC4; replication factor C subunit 4 [KO:K10755]
RfC3; replication factor C subunit 3 [KO:K10756]
RfC38; replication factor C 38kD subunit, isoform B [KO:K10756]
CG13690; uncharacterized protein [KO:K10743] [EC:3.1.26.4]
CG11164; uncharacterized protein, isoform B [KO:K10744]
CG30105; uncharacterized protein [KO:K10745]
Reference
PMID:8087839
  Authors
Stillman B.
  Title
Smart machines at the DNA replication fork.
  Journal
Cell 78:725-8 (1994)
DOI:10.1016/S0092-8674(94)90362-X
Reference
PMID:9759502
  Authors
Waga S, Stillman B.
  Title
The DNA replication fork in eukaryotic cells.
  Journal
Annu Rev Biochem 67:721-51 (1998)
DOI:10.1146/annurev.biochem.67.1.721
KO pathway
 

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