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dme03030                    Pathway                                

DNA replication - Drosophila melanogaster (fruit fly)
A complex network of interacting proteins and enzymes is required for DNA replication. Generally, DNA replication follows a multistep enzymatic pathway. At the DNA replication fork, a DNA helicase (DnaB or MCM complex) precedes the DNA synthetic machinery and unwinds the duplex parental DNA in cooperation with the SSB or RPA. On the leading strand, replication occurs continuously in a 5 to 3 direction, whereas on the lagging strand, DNA replication occurs discontinuously by synthesis and joining of short Okazaki fragments. In prokaryotes, the leading strand replication apparatus consists of a DNA polymerase (pol III core), a sliding clamp (beta), and a clamp loader (gamma delta complex). The DNA primase (DnaG) is needed to form RNA primers. Normally, during replication of the lagging-strand DNA template, an RNA primer is removed either by an RNase H or by the 5 to 3 exonuclease activity of DNA pol I, and the DNA ligase joins the Okazaki fragments. In eukaryotes, three DNA polymerases (alpha, delta, and epsilon) have been identified. DNA primase forms a permanent complex with DNA polymerase alpha. PCNA and RFC function as a clamp and a clamp loader. FEN 1 and RNase H1 remove the RNA from the Okazaki fragments and DNA ligase I joins the DNA.
Genetic Information Processing; Replication and repair
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DNA replication

All organismsOrtholog table
DNA polymerase alpha / primase complex [PATH:dme03030]
DNA polymerase epsilon complex [PATH:dme03030]
MCM complex [PATH:dme03030]
RPA complex [PATH:dme03030]
RF-C complex [PATH:dme03030]
Drosophila melanogaster (fruit fly) [GN:dme]
mtSSB; mitochondrial single stranded DNA-binding protein [KO:K03111]
rnh1; ribonuclease H1, isoform A [KO:K03469] [EC:]
RpA-70; replication protein A 70 [KO:K07466]
PCNA; proliferating cell nuclear antigen, isoform A [KO:K04802]
CG10262; uncharacterized protein [KO:K04802]
CG2990, isoform C [KO:K10742] [EC:]
Fen1; flap endonuclease 1 [KO:K04799] [EC:3.-.-.-]
DNA-ligI; DNA ligase I [KO:K10747] [EC:]
DNApol-alpha180; DNA polymerase alpha 180kD [KO:K02320] [EC:]
DNApol-alpha73; DNA polymerase alpha 73kD [KO:K02321]
DNApol-alpha50; DNA polymerase alpha 50kD [KO:K02684] [EC:2.7.7.-]
DNApol-alpha60; DNA polymerase alpha 60kD [KO:K02685] [EC:2.7.7.-]
DNApol-delta; DNA-polymerase-delta [KO:K02327] [EC:]
CG12018; uncharacterized protein [KO:K02328]
Pol32; Pol32 polymerase delta subunit, isoform B [KO:K03504]
DNApol-epsilon255; DNA polymerase epsilon 255kD subunit [KO:K02324] [EC:]
DNApol-epsilon58; DNA polymerase epsilon 58kD subunit [KO:K02325] [EC:]
Chrac-14; chromatin accessibility complex 14kD protein [KO:K02326] [EC:]
Mes4; Mesoderm-expressed 4, isoform B [KO:K03506] [EC:]
Mcm2; minichromosome maintenance 2 [KO:K02540] [EC:]
Mcm3; minichromosome maintenance 3, isoform A [KO:K02541] [EC:]
dpa; disc proliferation abnormal, isoform B [KO:K02212] [EC:]
Mcm5; minichromosome maintenance 5 [KO:K02209] [EC:]
Mcm6; minichromosome maintenance 6 [KO:K02542] [EC:]
Mcm7; minichromosome maintenance 7 [KO:K02210] [EC:]
RPA2; replication protein A2, isoform B [KO:K10739]
RPA3; replication protein A3, isoform B [KO:K10740]
Gnf1; germ line transcription factor 1, isoform A [KO:K10754]
CG8142 [KO:K10755]
RfC4; replication factor C subunit 4 [KO:K10755]
RfC3; replication factor C subunit 3 [KO:K10756]
RfC38; replication factor C 38kD subunit, isoform B [KO:K10756]
CG13690; uncharacterized protein [KO:K10743] [EC:]
CG11164; uncharacterized protein, isoform B [KO:K10744]
CG30105; uncharacterized protein [KO:K10745]
Stillman B.
Smart machines at the DNA replication fork.
Cell 78:725-8 (1994)
Waga S, Stillman B.
The DNA replication fork in eukaryotic cells.
Annu Rev Biochem 67:721-51 (1998)
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