KEGG   ENZYME: 1.1.1.272Help
Entry
EC 1.1.1.272                Enzyme                                 

Name
D-2-hydroxyacid dehydrogenase (NADP+);
ddh (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(R)-2-hydroxycarboxylate:NADP+ oxidoreductase
Reaction(IUBMB)
an (R)-2-hydroxycarboxylate + NADP+ = a 2-oxocarboxylate + NADPH + H+ [RN:R10528]
Reaction(KEGG)
Substrate
(R)-2-hydroxycarboxylate [CPD:C02489];
NADP+ [CPD:C00006]
Product
2-oxocarboxylate [CPD:C00161];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
This enzyme, characterized from the halophilic archaeon Haloferax mediterranei and the mold Aspergillus oryzae, catalyses a stereospecific reduction of 2-oxocarboxylic acids into the corresponding D-2-hydroxycarboxylic acids. The enzyme prefers substrates with a main chain of 5 carbons (such as 4-methyl-2-oxopentanoate) to those with a shorter chain, and can use NADH with much lower efficiency. cf. EC 1.1.1.345, (D)-2-hydroxyacid dehydrogenase (NAD+).
History
EC 1.1.1.272 created 2002, modified 2013
Orthology
K17064  
D-2-hydroxyacid dehydrogenase (NADP+)
Genes
HMA: 
rrnAC2451(serA5)
HHI: 
HAH_2884(serA4)
HHN: 
NMO: 
HJE: 
HVO: 
HME: 
HFX_2024(serA5)
HBO: 
HTU: 
NMG: 
HXA: 
NAT: 
NPE: 
NOU: 
SALI: 
 » show all
Taxonomy
Reference
1  [PMID:17049749]
  Authors
Domenech J, Ferrer J
  Title
A new D-2-hydroxyacid dehydrogenase with dual coenzyme-specificity from Haloferax mediterranei, sequence analysis and heterologous overexpression.
  Journal
Biochim. Biophys. Acta. 1760 (2006) 1667-74.
  Sequence
[hme:HFX_2024]
Reference
2  [PMID:26615399]
  Authors
Shimizu M, Yamamoto T, Okabe N, Sakai K, Koide E, Miyachi Y, Kurimoto M, Mochizuki M, Yoshino-Yasuda S, Mitsui S, Ito A, Murano H, Takaya N, Kato M
  Title
Novel 4-methyl-2-oxopentanoate reductase involved in synthesis of the Japanese sake flavor, ethyl leucate.
  Journal
Appl. Microbiol. Biotechnol. 100 (2016) 3137-45.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
81210-65-3

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