KEGG   ENZYME: 1.1.1.312Help
Entry
EC 1.1.1.312                Enzyme                                 

Name
2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase;
2-hydroxy-4-carboxymuconate 6-semialdehyde dehydrogenase;
4-carboxy-2-hydroxy-cis,cis-muconate-6-semialdehyde:NADP+ oxidoreductase;
alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase;
4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase;
LigC;
ProD
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal:NADP+ 2-oxidoreductase
Reaction(IUBMB)
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal + NADP+ = 2-oxo-2H-pyran-4,6-dicarboxylate + NADPH + H+ [RN:R04279]
Reaction(KEGG)
Substrate
4-carboxy-2-hydroxymuconate semialdehyde hemiacetal;
NADP+ [CPD:C00006]
Product
2-oxo-2H-pyran-4,6-dicarboxylate;
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme does not act on unsubstituted aliphatic or aromatic aldehydes or glucose; NAD+ can replace NADP+, but with lower affinity. The enzyme was initially believed to act on 4-carboxy-2-hydroxy-cis,cis-muconate 6-semialdehyde and produce 4-carboxy-2-hydroxy-cis,cis-muconate [1]. However, later studies showed that the substrate is the hemiacetal form [3], and the product is 2-oxo-2H-pyran-4,6-dicarboxylate [2,4].
History
EC 1.1.1.312 created 1978 as EC 1.2.1.45, transferred 2011 to EC 1.1.1.312
Pathway
Tyrosine metabolism
Benzoate degradation
Aminobenzoate degradation
Orthology
K10219  
2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase
Genes
VEJ: 
PAT: 
CSA: 
MMW: 
MME: 
MPC: 
GSN: 
BPH: 
BPX: 
CDN: 
RFR: 
PNA: 
ACK: 
VEI: 
DAC: 
DEL: 
CTT: 
CTES: 
RTA: 
LIM: 
LIH: 
CBAA: 
CBAB: 
LCH: 
AZO: 
azo2536(ligC)
THU: 
SHD: 
LLU: 
BRA: 
BRADO2343(ligC)
BBT: 
BBta_2703(ligC)
AOL: 
RPA: 
RPA4703(ligC)
RPB: 
RPD: 
RPE: 
RPT: 
RPX: 
RHZ: 
BSB: 
MALG: 
NAR: 
NPP: 
NPN: 
SPHI: 
SSAN: 
SCH: 
SSY: 
SLG_12490(ligC)
AAY: 
WYH_01910(ligC)
MAG: 
MAGX: 
XM1_1092(ligC)
ABS: 
ABQ: 
MAGQ: 
APB: 
KSK: 
KSE_75130(pcmB)
ART: 
APN: 
DPD: 
 » show all
Taxonomy
Reference
1  [PMID:26671]
  Authors
Maruyama K, Ariga N, Tsuda M, Deguchi K.
  Title
Purification and properties of alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase.
  Journal
J. Biochem. (Tokyo). 83 (1978) 1125-34.
Reference
2  [PMID:528534]
  Authors
Maruyama K
  Title
Isolation and identification of the reaction product of alpha-hydroxy-gamma-carboxymuconic epsilon-semialdehyde dehydrogenase.
  Journal
J. Biochem. 86 (1979) 1671-7.
Reference
3  [PMID:6841353]
  Authors
Maruyama K.
  Title
Purification and properties of 2-pyrone-4,6-dicarboxylate hydrolase.
  Journal
J. Biochem. (Tokyo). 93 (1983) 557-65.
Reference
4  [PMID:11073908]
  Authors
Masai E, Momose K, Hara H, Nishikawa S, Katayama Y, Fukuda M
  Title
Genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in Sphingomonas paucimobilis SYK-6.
  Journal
J. Bacteriol. 182 (2000) 6651-8.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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