KEGG   ENZYME: 1.1.1.314Help
Entry
EC 1.1.1.314                Enzyme                                 

Name
germacrene A alcohol dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
germacra-1(10),4,11(13)-trien-12-ol:NADP+ oxidoreductase
Reaction(IUBMB)
germacra-1(10),4,11(13)-trien-12-ol + 2 NADP+ + H2O = germacra-1(10),4,11(13)-trien-12-oate + 2 NADPH + 3 H+ (overall reaction) [RN:R09548];
(1a) germacra-1(10),4,11(13)-trien-12-ol + NADP+ = germacra-1(10),4,11(13)-trien-12-al + NADPH + H+ [RN:R09546];
(1b) germacra-1(10),4,11(13)-trien-12-al + NADP+ + H2O = germacra-1(10),4,11(13)-trien-12-oate + NADPH + 2 H+ [RN:R09547]
Reaction(KEGG)
Substrate
germacra-1(10),4,11(13)-trien-12-ol [CPD:C19676];
NADP+ [CPD:C00006];
H2O [CPD:C00001];
germacra-1(10),4,11(13)-trien-12-al [CPD:C19677]
Product
germacra-1(10),4,11(13)-trien-12-oate [CPD:C19678];
NADPH [CPD:C00005];
H+ [CPD:C00080];
germacra-1(10),4,11(13)-trien-12-al [CPD:C19677]
Comment
In Lactuca sativa EC 1.1.1.314 is a mutifunctional enzyme with EC 1.14.13.123, germacrene A hydroxylase [2].
History
EC 1.1.1.314 created 2011
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K15800  
germacrene A oxidase
Reference
1  [PMID:11299372]
  Authors
de Kraker JW, Franssen MC, Dalm MC, de Groot A, Bouwmeester HJ
  Title
Biosynthesis of germacrene A carboxylic acid in chicory roots. Demonstration of a cytochrome P450 (+)-germacrene a hydroxylase and NADP+-dependent sesquiterpenoid  dehydrogenase(s) involved in sesquiterpene lactone biosynthesis.
  Journal
Plant. Physiol. 125 (2001) 1930-40.
Reference
2  [PMID:20351109]
  Authors
Nguyen DT, Gopfert JC, Ikezawa N, Macnevin G, Kathiresan M, Conrad J, Spring O, Ro DK
  Title
Biochemical conservation and evolution of germacrene A oxidase in asteraceae.
  Journal
J. Biol. Chem. 285 (2010) 16588-98.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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