KEGG   ENZYME: 1.1.1.319Help
Entry
EC 1.1.1.319                Enzyme                                 

Name
isoeugenol synthase;
IGS1;
t-anol/isoeugenol synthase 1
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
eugenol:NADP+ oxidoreductase (coniferyl acetate reducing)
Reaction(IUBMB)
isoeugenol + acetate + NADP+ = coniferyl acetate + NADPH + H+ [RN:R09931]
Reaction(KEGG)
R09931;
(other) R10289
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Substrate
isoeugenol [CPD:C10469];
acetate [CPD:C00033];
NADP+ [CPD:C00006]
Product
coniferyl acetate [CPD:C20225];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme acts in the opposite direction. In Ocimum basilicum (sweet basil), Clarkia breweri and Petunia hybrida only isoeugenol is formed [1,2]. However in Pimpinella anisum (anise) only anol is formed in vivo, although the cloned enzyme does produce isoeugenol [3].
History
EC 1.1.1.319 created 2012
Pathway
Phenylpropanoid biosynthesis
Orthology
K17056  
isoeugenol synthase
Reference
1  [PMID:16782809]
  Authors
Koeduka T, Fridman E, Gang DR, Vassao DG, Jackson BL, Kish CM, Orlova I, Spassova SM, Lewis NG, Noel JP, Baiga TJ, Dudareva N, Pichersky E
  Title
Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 10128-33.
  Organism
Ocimum basilicum
  Sequence
[up:Q15GI3]
Reference
2  [PMID:18208524]
  Authors
Koeduka T, Louie GV, Orlova I, Kish CM, Ibdah M, Wilkerson CG, Bowman ME, Baiga TJ, Noel JP, Dudareva N, Pichersky E
  Title
The multiple phenylpropene synthases in both Clarkia breweri and Petunia hybrida  represent two distinct protein lineages.
  Journal
Plant. J. 54 (2008) 362-74.
  Organism
Clarkia breweri
Reference
3  [PMID:18987218]
  Authors
Koeduka T, Baiga TJ, Noel JP, Pichersky E
  Title
Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain.
  Journal
Plant. Physiol. 149 (2009) 384-94.
  Organism
Pimpinella anisum
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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