KEGG   ENZYME: 1.1.1.334
Entry
EC 1.1.1.334                Enzyme                                 
Name
methylecgonone reductase;
MecgoR (gene name)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
ecgonine methyl ester:NADP+ oxidoreductase
Reaction(IUBMB)
ecgonine methyl ester + NADP+ = ecgonone methyl ester + NADPH + H+ [RN:R10114]
Reaction(KEGG)
R10114
Substrate
ecgonine methyl ester [CPD:C12448];
NADP+ [CPD:C00006]
Product
ecgonone methyl ester [CPD:C20371];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme from the plant Erythroxylum coca catalyses the penultimate step in the biosynthesis of cocaine. In vivo the reaction proceeds in the opposite direction. With NADH instead of NADPH the reaction rate is reduced to 14%. The enzyme also reduces tropinone, nortropinone and 6-hydroxytropinone but with lower reaction rates.
History
EC 1.1.1.334 created 2012
Orthology
K19651  methylecgonone reductase
Reference
1  [PMID:22665766]
  Authors
Jirschitzka J, Schmidt GW, Reichelt M, Schneider B, Gershenzon J, D'Auria JC
  Title
Plant tropane alkaloid biosynthesis evolved independently in the Solanaceae and Erythroxylaceae.
  Journal
Proc Natl Acad Sci U S A 109:10304-9 (2012)
DOI:10.1073/pnas.1200473109
  Sequence
[ag:ADK94763]
Other DBs
ExplorEnz - The Enzyme Database: 1.1.1.334
IUBMB Enzyme Nomenclature: 1.1.1.334
ExPASy - ENZYME nomenclature database: 1.1.1.334
BRENDA, the Enzyme Database: 1.1.1.334

  All links  
Chemical substance (5)   
   KEGG COMPOUND (5)   
Chemical reaction (2)   
   KEGG REACTION (1)   
   KEGG RCLASS (1)   
Gene (2)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (1)   
Protein sequence (19)   
   UniProt (18)   
   SWISS-PROT (1)   
Protein domain (5)   
   InterPro (5)   
All databases (33)   

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