KEGG   ENZYME: 1.1.1.348Help
Entry
EC 1.1.1.348                Enzyme                                 

Name
vestitone reductase;
pterocarpin synthase (incorrect);
pterocarpan synthase (incorrect)
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol:NADP+ 4-oxidoreductase
Reaction(IUBMB)
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol + NADP+ = (3R)-vestitone + NADPH + H+ [RN:R07737]
Reaction(KEGG)
Substrate
(3R,4R)-4'-methoxyisoflavan-2',4,7-triol [CPD:C16187];
NADP+ [CPD:C00006]
Product
(3R)-vestitone;
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
This plant enzyme catalyses the penultimate step in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain. This activity was previously classified as part of EC 1.1.1.246, pterocarpin synthase, which is now known to be catalysed by two enzymes, vestitone reductase and EC 4.2.1.139, medicarpin synthase.
History
EC 1.1.1.348 created 1992 as EC 1.1.1.246, part transferred to EC 1.1.1.348 2013
Pathway
Isoflavonoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K13265  
vestitone reductase
Genes
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
Taxonomy
Reference
1
  Authors
Bless, W. and Barz, W.
  Title
Isolation of pterocarpan synthase, the terminal enzyme of pterocarpan phytoalexin biosynthesis in cell-suspension cultures of Cicer arietinum.
  Journal
FEBS Lett. 235 (1988) 47-50.
Reference
2  [PMID:8071365]
  Authors
Guo L, Dixon RA, Paiva NL.
  Title
Conversion of vestitone to medicarpin in alfalfa (Medicago sativa L.) is catalyzed by two independent enzymes. Identification, purification, and characterization of vestitone reductase and 7,2'-dihydroxy-4'-methoxyisoflavanol  dehydratase.
  Journal
J. Biol. Chem. 269 (1994) 22372-8.
  Sequence
Reference
3  [PMID:7805842]
  Authors
Guo L, Dixon RA, Paiva NL
  Title
The 'pterocarpan synthase' of alfalfa: association and co-induction of vestitone  reductase and 7,2'-dihydroxy-4'-methoxy-isoflavanol (DMI) dehydratase, the two final enzymes in medicarpin biosynthesis.
  Journal
FEBS. Lett. 356 (1994) 221-5.
  Sequence
Reference
4  [PMID:7625843]
  Authors
Guo L, Paiva NL
  Title
Molecular cloning and expression of alfalfa (Medicago sativa L.) vestitone reductase, the penultimate enzyme in medicarpin biosynthesis.
  Journal
Arch. Biochem. Biophys. 320 (1995) 353-60.
  Sequence
Reference
5  [PMID:17433362]
  Authors
Shao H, Dixon RA, Wang X
  Title
Crystal structure of vestitone reductase from alfalfa (Medicago sativa L.).
  Journal
J. Mol. Biol. 369 (2007) 265-76.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
118477-70-6

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