KEGG   ENZYME: 1.1.1.375Help
Entry
EC 1.1.1.375                Enzyme                                 

Name
L-2-hydroxycarboxylate dehydrogenase [NAD(P)+];
MdhII;
lactate/malate dehydrogenase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
(2S)-2-hydroxycarboxylate:NAD(P)+ oxidoreductase
Reaction(IUBMB)
a (2S)-2-hydroxycarboxylate + NAD(P)+ = a 2-oxocarboxylate + NAD(P)H + H+ [RN:R10189 R10786]
Reaction(KEGG)
Substrate
(2S)-2-hydroxycarboxylate [CPD:C15565];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006]
Product
2-oxocarboxylate [CPD:C00161];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme from the archaeon Methanocaldococcus jannaschii catalyses the reversible oxidation of (2R)-3-sulfolactate and (S)-malate to 3-sulfopyruvate and oxaloacetate, respectively (note that (2R)-3-sulfolactate has the same stereochemical configuration as (2S)-2-hydroxycarboxylates) [1]. The enzyme can use both NADH and NADPH, although activity is higher with NADPH [1-3]. The oxidation of (2R)-3-sulfolactate was observed only in the presence of NADP+ [1]. The same organism also possesses an NAD+-specific enzyme with similar activity, cf. EC 1.1.1.337, L-2-hydroxycarboxylate dehydrogenase (NAD+).
History
EC 1.1.1.375 created 2014
Reference
1  [PMID:10850983]
  Authors
Graupner M, Xu H, White RH.
  Title
Identification of an archaeal 2-hydroxy acid dehydrogenase catalyzing reactions involved in coenzyme biosynthesis in methanoarchaea.
  Journal
J. Bacteriol. 182 (2000) 3688-92.
  Sequence
[mja:MJ_0490]
Reference
2  [PMID:11292347]
  Authors
Lee BI, Chang C, Cho SJ, Eom SH, Kim KK, Yu YG, Suh SW
  Title
Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases.
  Journal
J. Mol. Biol. 307 (2001) 1351-62.
  Sequence
[mja:MJ_0490]
Reference
3  [PMID:10998181]
  Authors
Madern D
  Title
The putative L-lactate dehydrogenase from Methanococcus jannaschii is an NADPH-dependent L-malate dehydrogenase.
  Journal
Mol. Microbiol. 37 (2000) 1515-20.
  Sequence
[mja:MJ_0490]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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