KEGG   ENZYME: 1.1.3.28Help
Entry
EC 1.1.3.28                 Enzyme                                 

Name
nucleoside oxidase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
nucleoside:oxygen 5'-oxidoreductase
Reaction(IUBMB)
inosine + O2 = 9-riburonosylhypoxanthine + H2O [RN:R05821];
(1a) 2 inosine + O2 = 2 5'-dehydroinosine + 2 H2O [RN:R00055];
(1b) 2 5'-dehydroinosine + O2 = 2 9-riburonosylhypoxanthine [RN:R05822]
Reaction(KEGG)
Substrate
inosine [CPD:C00294];
O2 [CPD:C00007];
5'-dehydroinosine [CPD:C01993]
Product
9-riburonosylhypoxanthine [CPD:C11631];
H2O [CPD:C00001];
5'-dehydroinosine [CPD:C01993]
Comment
Other purine and pyrimidine nucleosides (as well as 2'-deoxyribonucleosides) are substrates, but ribose and nucleotides are not substrates. The overall reaction takes place in two separate steps, with the 5'-dehydro nucleoside being released from the enzyme to serve as substrate for the second reaction. This enzyme differs from EC 1.1.3.39, nucleoside oxidase (H2O2-forming), as it produces water rather than hydrogen peroxide.
History
EC 1.1.3.28 created 1992, modified 2001
Reference
1
  Authors
Isono, Y., Sudo, T. and Hoshino, M.
  Title
Purification and reaction of a new enzyme, nucleoside oxidase.
  Journal
Agric. Biol. Chem. 53 (1989) 1663-1669.
Reference
2
  Authors
Isono, Y., Sudo, T. and Hoshino, M.
  Title
Properties of a new enzyme, nucleoside oxidase, from Pseudomonas maltophilia LB-86.
  Journal
Agric. Biol. Chem. 53 (1989) 1671-1677.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
82599-71-1

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