KEGG   ENZYME: 1.1.99.18Help
Entry
EC 1.1.99.18                Enzyme                                 

Name
cellobiose dehydrogenase (acceptor);
cellobiose dehydrogenase;
cellobiose oxidoreductase;
Phanerochaete chrysosporium cellobiose oxidoreductase;
CBOR;
cellobiose oxidase;
cellobiose:oxygen 1-oxidoreductase;
CDH;
cellobiose:(acceptor) 1-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With other acceptors
BRITE hierarchy
Sysname
cellobiose:acceptor 1-oxidoreductase
Reaction(IUBMB)
cellobiose + acceptor = cellobiono-1,5-lactone + reduced acceptor [RN:R01443 R06246]
Reaction(KEGG)
R01443 R06246(G) > R01442 R06244(G);
(other) R02365 R06247(G)
Show
Substrate
cellobiose [CPD:C00185];
acceptor [CPD:C00028]
Product
cellobiono-1,5-lactone [CPD:C01093];
reduced acceptor [CPD:C00030]
Comment
2,6-Dichloroindophenol can act as acceptor. Also acts, more slowly, on cello-oligosaccharides, lactose and D-glucosyl-1,4-beta-D-mannose. Includes EC 1.1.5.1, cellobiose dehydrogenase (quinone), which is now known to be a proteolytic product of this enzyme. The enzyme from the white rot fungus Phanerochaete chrysosporium is unusual in having two redoxin domains, one containing a flavin and the other a protoheme group. It transfers reducing equivalents from cellobiose to two types of redox acceptor: two-electron oxidants, including redox dyes, benzoquinones and molecular oxygen and one-electron oxidants, including semiquinone species, iron(II) complexes and the model acceptor cytochrome c [9].
History
EC 1.1.99.18 created 1983, modified 2002 (EC 1.1.5.1 created 1983, incorporated 2002; EC 1.1.3.25 created 1986, incorporated 2005)
Reference
1  [PMID:7103940]
  Authors
Coudray MR, Canevascini G, Meier H.
  Title
Characterization of a cellobiose dehydrogenase in the cellulolytic fungus Sporotrichum (Chrysosporium) thermophile.
  Journal
Biochem. J. 203 (1982) 277-84.
  Organism
Sporotrichum thermophile
Reference
2
  Authors
Dekker, R.F.H.
  Title
Induction and characterization of a cellobiose dehydrogenase produced by a species of Monilia.
  Journal
J. Gen. Microbiol. 120 (1980) 309-316.
Reference
3
  Authors
Dekker, R.F.H.
  Title
Cellobiose dehydrogenase produced by Monilia sp.
  Journal
Methods Enzymol. 160 (1988) 454-463.
Reference
4  [PMID:8392950]
  Authors
Habu N, Samejima M, Dean JF, Eriksson KE.
  Title
Release of the FAD domain from cellobiose oxidase by proteases from cellulolytic cultures of Phanerochaete chrysosporium.
  Journal
FEBS. Lett. 327 (1993) 161-4.
  Organism
Phanerochaete chrysosporium
Reference
5  [PMID:11282631]
  Authors
Baminger U, Subramaniam SS, Renganathan V, Haltrich D.
  Title
Purification and characterization of cellobiose dehydrogenase from the plant pathogen Sclerotium (Athelia) rolfsii.
  Journal
Appl. Environ. Microbiol. 67 (2001) 1766-74.
  Organism
Sclerotium rolfsii
Reference
6  [PMID:11786022]
  Authors
Hallberg BM, Henriksson G, Pettersson G, Divne C.
  Title
Crystal structure of the flavoprotein domain of the extracellular flavocytochrome cellobiose dehydrogenase.
  Journal
J. Mol. Biol. 315 (2002) 421-34.
  Organism
Phanerochaete chrysosporium
Reference
7  [PMID:710416]
  Authors
Ayers AR, Ayers SB, Eriksson KE.
  Title
Cellobiose oxidase, purification and partial characterization of a hemoprotein from Sporotrichum pulverulentum.
  Journal
Eur. J. Biochem. 90 (1978) 171-81.
  Organism
Sporotrichum pulverulentum
Reference
8  [PMID:7144569]
  Authors
Ayers AR, Eriksson KE.
  Title
Cellobiose oxidase from Sporotrichum pulverulentum.
  Journal
Methods. Enzymol. 89 Pt D (1982) 129-35.
  Organism
Sporotrichum pulverulentum
Reference
9  [PMID:12686420]
  Authors
Mason MG, Nicholls P, Divne C, Hallberg BM, Henriksson G, Wilson MT.
  Title
The heme domain of cellobiose oxidoreductase: a one-electron reducing system.
  Journal
Biochim. Biophys. Acta. 1604 (2003) 47-54.
  Organism
Phanerochaete chrysosporium
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
54576-85-1

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