KEGG   ENZYME: 1.1.99.29Help
Entry
EC 1.1.99.29                Enzyme                                 

Name
pyranose dehydrogenase (acceptor);
pyranose dehydrogenase;
pyranose-quinone oxidoreductase;
quinone-dependent pyranose dehydrogenase;
PDH
Class
Oxidoreductases;
Acting on the CH-OH group of donors;
With other acceptors
BRITE hierarchy
Sysname
pyranose:acceptor oxidoreductase
Reaction(IUBMB)
(1) a pyranose + acceptor = a pyranos-2-ulose (or a pyranos-3-ulose or a pyranos-2,3-diulose) + reduced acceptor [RN:R07347];
(2) a pyranoside + acceptor = a pyranosid-3-ulose (or a pyranosid-3,4-diulose) + reduced acceptor [RN:R07348]
Reaction(KEGG)
Substrate
pyranose [CPD:C15592];
acceptor [CPD:C00028];
pyranoside [CPD:C15594]
Product
pyranos-2-ulose;
pyranos-3-ulose;
pyranos-2,3-diulose;
reduced acceptor [CPD:C00030];
pyranosid-3-ulose;
pyranosid-3,4-diulose
Comment
Requires FAD. A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. Unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. D-Glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. Sugars are oxidized in their pyranose but not in their furanose form.
History
EC 1.1.99.29 created 2004
Reference
1  [PMID:9042751]
  Authors
Volc J, Kubatova E, Wood DA, Daniel G.
  Title
Pyranose 2-dehydrogenase, a novel sugar oxidoreductase from the basidiomycete fungus Agaricus bisporus
  Journal
Arch. Microbiol. 167 (1997) 119-25.
  Organism
Agaricus bisporus
Reference
2
  Authors
Volc, J., Sedmera, P., Halada, P., Poikyrlova, V. and Daniel, G.
  Title
C-2 and C-3 oxidation of D-Glc, and C-2 oxidation of D-Gal by pyranose dehydrogenase from Agaricus bisporus.
  Journal
Carbohydr. Res. 310 (1998) 151-156.
Reference
3  [PMID:11086703]
  Authors
Volc J, Sedmera P, Halada P, Prikrylova V, Haltrich D.
  Title
Double oxidation of D-xylose to D-glycero -pentos-2,3-diulose (2,3-diketo-D-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus.
  Journal
Carbohydr. Res. 329 (2000) 219-25.
  Organism
Agaricus bisporus
Reference
4  [PMID:11511865]
  Authors
Volc J, Kubatova E, Daniel G, Sedmera P, Haltrich D.
  Title
Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes.
  Journal
Arch. Microbiol. 176 (2001) 178-86.
  Organism
Macrolepiota rhacodes
Reference
5
  Authors
Volc, J., Sedmera, P., Halada, P., Daniel, G., Poikyrlova, V. and Haltrich, D.
  Title
C-3 oxidation of non-reducing sugars by a fungal pyranose dehydrogenase: spectral characterization.
  Journal
J. Mol. Catal., B Enzym. 17 (2002) 91-100.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
190606-21-4

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