pyranose dehydrogenase (acceptor);
pyranose dehydrogenase;
pyranose-quinone oxidoreductase;
quinone-dependent pyranose dehydrogenase;
PDH

Oxidoreductases;
Acting on the CH-OH group of donors;
With other acceptors

pyranose:acceptor oxidoreductase

(1) a pyranose + acceptor = a pyranos-2-ulose (or a pyranos-3-ulose or a pyranos-2,3-diulose) + reduced acceptor [RN:R07347];
(2) a pyranoside + acceptor = a pyranosid-3-ulose (or a pyranosid-3,4-diulose) + reduced acceptor [RN:R07348]

R07347 R07348

pyranose [CPD:C15592];
acceptor [CPD:C00028];
pyranoside [CPD:C15594]

Requires FAD. A number of aldoses and ketoses in pyranose form, as well as glycosides, gluco-oligosaccharides, sucrose and lactose can act as a donor. 1,4-Benzoquinone or ferricenium ion (ferrocene oxidized by removal of one electron) can serve as acceptor. Unlike EC 1.1.3.10, pyranose oxidase, this fungal enzyme does not interact with O2 and exhibits extremely broad substrate tolerance with variable regioselectivity (C-3, C-2 or C-3 + C-2 or C-3 + C-4) for (di)oxidation of different sugars. D-Glucose is exclusively or preferentially oxidized at C-3 (depending on the enzyme source), but can also be oxidized at C-2 + C-3. The enzyme also acts on 1->4-alpha- and 1->4-beta-gluco-oligosaccharides, non-reducing gluco-oligosaccharides and L-arabinose, which are not substrates of EC 1.1.3.10. Sugars are oxidized in their pyranose but not in their furanose form.

1  [PMID:9042751]

Volc J, Kubatova E, Wood DA, Daniel G.

Pyranose 2-dehydrogenase, a novel sugar oxidoreductase from the basidiomycete fungus Agaricus bisporus

Arch. Microbiol. 167 (1997) 119-25.

Agaricus bisporus

2

Volc, J., Sedmera, P., Halada, P., Poikyrlova, V. and Daniel, G.

C-2 and C-3 oxidation of D-Glc, and C-2 oxidation of D-Gal by pyranose dehydrogenase from Agaricus bisporus.

Carbohydr. Res. 310 (1998) 151-156.

3  [PMID:11086703]

Volc J, Sedmera P, Halada P, Prikrylova V, Haltrich D.

Double oxidation of D-xylose to D-glycero -pentos-2,3-diulose (2,3-diketo-D-xylose) by pyranose dehydrogenase from the mushroom Agaricus bisporus.

Carbohydr. Res. 329 (2000) 219-25.

Agaricus bisporus

4  [PMID:11511865]

Volc J, Kubatova E, Daniel G, Sedmera P, Haltrich D.

Screening of basidiomycete fungi for the quinone-dependent sugar C-2/C-3 oxidoreductase, pyranose dehydrogenase, and properties of the enzyme from Macrolepiota rhacodes.

Arch. Microbiol. 176 (2001) 178-86.

Macrolepiota rhacodes

5

Volc, J., Sedmera, P., Halada, P., Daniel, G., Poikyrlova, V. and Haltrich, D.

C-3 oxidation of non-reducing sugars by a fungal pyranose dehydrogenase: spectral characterization.

J. Mol. Catal., B Enzym. 17 (2002) 91-100.