| Entry |
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| Name |
alcohol dehydrogenase (nicotinoprotein);
NDMA-dependent alcohol dehydrogenase;
nicotinoprotein alcohol dehydrogenase;
np-ADH;
ethanol:N,N-dimethyl-4-nitrosoaniline oxidoreductase
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| Class |
Oxidoreductases;
Acting on the CH-OH group of donors;
With other acceptors
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| Sysname |
ethanol:acceptor oxidoreductase
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| Reaction(IUBMB) |
ethanol + acceptor = acetaldehyde + reduced acceptor [RN: R09552]
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| Reaction(KEGG) |
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| Substrate |
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| Product |
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| Comment |
Contains Zn2+. Nicotinoprotein alcohol dehydrogenases are unique medium-chain dehydrogenases/reductases (MDR) alcohol dehydrogenases that have a tightly bound NAD+/NADH cofactor that does not dissociate during the catalytic process. Instead, the cofactor is regenerated by a second substrate or electron carrier. While the in vivo electron acceptor is not known, N,N-dimethyl-4-nitrosoaniline (NDMA), which is reduced to 4-(hydroxylamino)-N,N-dimethylaniline, can serve this function in vitro.
The enzyme from the Gram-positive bacterium Amycolatopsis methanolica can accept many primary alcohols as substrates, including benzylalcohol [1].
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| Reference |
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| Authors |
Van Ophem PW, Van Beeumen J, Duine JA |
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| Title |
Nicotinoprotein [NAD(P)-containing] alcohol/aldehyde oxidoreductases. Purification and characterization of a novel type from Amycolatopsis methanolica. |
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| Journal |
Eur. J. Biochem. 212 (1993) 819-26. |
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| Organism |
Amycolatopsis methanolica |
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| Reference |
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| Authors |
Piersma SR, Visser AJ, de Vries S, Duine JA |
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| Title |
Optical spectroscopy of nicotinoprotein alcohol dehydrogenase from Amycolatopsis methanolica: a comparison with horse liver alcohol dehydrogenase and UDP-galactose epimerase. |
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| Journal |
Biochemistry. 37 (1998) 3068-77. |
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| Organism |
Amycolatopsis methanolica |
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| Reference |
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| Authors |
Schenkels P, Duine JA |
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| Title |
Nicotinoprotein (NADH-containing) alcohol dehydrogenase from Rhodococcus erythropolis DSM 1069: an efficient catalyst for coenzyme-independent oxidation of a broad spectrum of alcohols and the interconversion of alcohols and aldehydes. |
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| Journal |
Microbiology. 146 ( Pt 4) (2000) 775-85. |
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| Organism |
Rhodococcus erythropolis [GN: rer] |
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| Reference |
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| Authors |
Piersma SR, Norin A, de Vries S, Jornvall H, Duine JA |
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| Title |
Inhibition of nicotinoprotein (NAD+-containing) alcohol dehydrogenase by trans-4-(N,N-dimethylamino)-cinnamaldehyde binding to the active site. |
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| Journal |
J. Protein. Chem. 22 (2003) 457-61. |
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| Organism |
Amycolatopsis methanolica |
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| Reference |
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| Authors |
Norin A, Piersma SR, Duine JA, Jornvall H |
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| Title |
Nicotinoprotein (NAD+ -containing) alcohol dehydrogenase: structural relationships and functional interpretations. |
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| Journal |
Cell. Mol. Life. Sci. 60 (2003) 999-1006. |
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| Organism |
Amycolatopsis methanolica |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: |
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