KEGG   ENZYME: 1.10.3.10Help
Entry
EC 1.10.3.10                Enzyme                                 

Name
ubiquinol oxidase (H+-transporting);
cytochrome bb3 oxidase;
cytochrome bo oxidase;
cytochrome bd-I oxidase;
cytochrome bd-II oxidase
Class
Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
ubiquinol:O2 oxidoreductase (H+-transporting)
Reaction(IUBMB)
2 ubiquinol + O2 + n H+[side 1] = 2 ubiquinone + 2 H2O + n H+[side 2] [RN:R09504]
Reaction(KEGG)
Substrate
ubiquinol [CPD:C00390];
O2 [CPD:C00007];
H+[side 1]
Product
ubiquinone [CPD:C00399];
H2O [CPD:C00001];
H+[side 2]
Comment
Contains a dinuclear centre comprising two hemes, or heme and copper. Reduction of O2 on the periplasmic side of the membrane leads to release of protons in the periplasm, generating a transmembrane proton gradient. In addition, the cytochrome bb3 and cytochrome bo oxidases, but not the cytochrome bd oxidases, contribute to the proton gradient by actively pumping protons across the membrane.
History
EC 1.10.3.10 created 2011
Reference
1  [PMID:11017202]
  Authors
Abramson J, Riistama S, Larsson G, Jasaitis A, Svensson-Ek M, Laakkonen L, Puustinen A, Iwata S, Wikstrom M
  Title
The structure of the ubiquinol oxidase from Escherichia coli and its ubiquinone binding site.
  Journal
Nat. Struct. Biol. 7 (2000) 910-7.
  Organism
Escherichia coli
  Sequence
[up:P0ABI8]
Reference
2  [PMID:15728392]
  Authors
Belevich I, Borisov VB, Zhang J, Yang K, Konstantinov AA, Gennis RB, Verkhovsky MI
  Title
Time-resolved electrometric and optical studies on cytochrome bd suggest a mechanism of electron-proton coupling in the di-heme active site.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 3657-62.
Reference
3  [PMID:20416270]
  Authors
Yap LL, Lin MT, Ouyang H, Samoilova RI, Dikanov SA, Gennis RB
  Title
The quinone-binding sites of the cytochrome bo3 ubiquinol oxidase from Escherichia coli.
  Journal
Biochim. Biophys. Acta. 1797 (2010) 1924-32.
Reference
4  [PMID:20392690]
  Authors
Shepherd M, Sanguinetti G, Cook GM, Poole RK
  Title
Compensations for diminished terminal oxidase activity in Escherichia coli: cytochrome bd-II-mediated respiration and glutamate metabolism.
  Journal
J. Biol. Chem. 285 (2010) 18464-72.
Reference
5  [PMID:21987791]
  Authors
Borisov VB, Murali R, Verkhovskaya ML, Bloch DA, Han H, Gennis RB, Verkhovsky MI
  Title
Aerobic respiratory chain of Escherichia coli is not allowed to work in fully uncoupled mode.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 108 (2011) 17320-4.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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