KEGG   ENZYME: 1.10.3.15Help
Entry
EC 1.10.3.15                Enzyme                                 

Name
grixazone synthase;
GriF
Class
Oxidoreductases;
Acting on diphenols and related substances as donors;
With oxygen as acceptor
BRITE hierarchy
Sysname
3-amino-4-hydroxybenzoate:N-acetyl-L-cysteine:oxygen oxidoreductase
Reaction(IUBMB)
2 3-amino-4-hydroxybenzoate + N-acetyl-L-cysteine + 2 O2 = grixazone B + 4 H2O + CO2 [RN:R10720]
Reaction(KEGG)
Substrate
3-amino-4-hydroxybenzoate [CPD:C12115];
N-acetyl-L-cysteine [CPD:C06809];
O2 [CPD:C00007]
Product
grixazone B [CPD:C20799];
H2O [CPD:C00001];
CO2 [CPD:C00011]
Comment
A type 3 multi copper protein. The enzyme, isolated from the bacterium Streptomyces griseus, catalyses an 8 electron oxidation. Activation of the enzyme requires a copper chaperone (GriE). It also acts on 3-amino-4-hydroxybenzaldehyde, giving grixazone A. The second aldehyde group is presumably lost as formate. The enzyme also catalyses the reaction of EC 1.10.3.4 o-aminophenol oxidase.
History
EC 1.10.3.15 created 2014
Orthology
K20204  
grixazone synthase / o-aminophenol oxidase
Genes
SGR: 
SGR_4246(griF)
Taxonomy
Reference
1  [PMID:17003031]
  Authors
Suzuki H, Ohnishi Y, Furusho Y, Sakuda S, Horinouchi S.
  Title
Novel benzene ring biosynthesis from C(3) and C(4) primary metabolites by two enzymes.
  Journal
J. Biol. Chem. 281 (2006) 36944-51.
  Sequence
[sgr:SGR_4246]
Reference
2  [PMID:19268377]
  Authors
Le Roes-Hill M, Goodwin C, Burton S
  Title
Phenoxazinone synthase: what's in a name?
  Journal
Trends. Biotechnol. 27 (2009) 248-58.
  Sequence
[sgr:SGR_4246]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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