KEGG   ENZYME: 1.11.1.16Help
Entry
EC 1.11.1.16                Enzyme                                 

Name
versatile peroxidase;
VP;
hybrid peroxidase;
polyvalent peroxidase
Class
Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases
BRITE hierarchy
Sysname
reactive-black-5:hydrogen-peroxide oxidoreductase
Reaction(IUBMB)
(1) Reactive Black 5 + H2O2 = oxidized Reactive Black 5 + 2 H2O [RN:R07612];
(2) donor + H2O2 = oxidized donor + 2 H2O [RN:R03532]
Reaction(KEGG)
Substrate
Reactive Black 5 [CPD:C16001];
H2O2 [CPD:C00027];
donor [CPD:C00030]
Product
oxidized Reactive Black 5 [CPD:C16002];
H2O [CPD:C00001];
oxidized donor [CPD:C00028]
Comment
A hemoprotein. This ligninolytic peroxidase combines the substrate-specificity characteristics of the two other ligninolytic peroxidases, EC 1.11.1.13, manganese peroxidase and EC 1.11.1.14, lignin peroxidase. It is also able to oxidize phenols, hydroquinones and both low- and high-redox-potential dyes, due to a hybrid molecular architecture that involves multiple binding sites for substrates [2,4].
History
EC 1.11.1.16 created 2006
Reference
1  [PMID:8647081]
  Authors
Martinez MJ, Ruiz-Duenas FJ, Guillen F, Martinez AT.
  Title
Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii.
  Journal
Eur. J. Biochem. 237 (1996) 424-32.
  Organism
Pleurotus eryngii
Reference
2  [PMID:9654123]
  Authors
Heinfling A, Ruiz-Duenas FJ, Martinez MJ, Bergbauer M, Szewzyk U, Martinez AT.
  Title
A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta.
  Journal
FEBS. Lett. 428 (1998) 141-6.
  Organism
Pleurotus eryngii, Bjerkandera adusta
Reference
3  [PMID:9987124]
  Authors
Ruiz-Duenas FJ, Martinez MJ, Martinez AT.
  Title
Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii.
  Journal
Mol. Microbiol. 31 (1999) 223-35.
  Organism
Pleurotus eryngii
  Sequence
Reference
4  [PMID:10187820]
  Authors
Camarero S, Sarkar S, Ruiz-Duenas FJ, Martinez MJ, Martinez AT.
  Title
Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites.
  Journal
J. Biol. Chem. 274 (1999) 10324-30.
  Organism
Pleurotus eryngii, Phanerochaete chrysosporium
  Sequence
[up:Q9UVP6]
Reference
5  [PMID:10508113]
  Authors
Ruiz-Duenas FJ, Martinez MJ, Martinez AT.
  Title
Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn(2+) and different aromatic substrates.
  Journal
Appl. Environ. Microbiol. 65 (1999) 4705-7.
  Organism
Pleurotus eryngii
Reference
6  [PMID:11004397]
  Authors
Camarero S, Ruiz-Duenas FJ, Sarkar S, Martinez MJ, Martinez AT.
  Title
The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
  Journal
FEMS. Microbiol. Lett. 191 (2000) 37-43.
  Organism
Pleurotus eryngii
  Sequence
[up:Q9UVP6]
Reference
7  [PMID:11356138]
  Authors
Ruiz-Duenas FJ, Camarero S, Perez-Boada M, Martinez MJ, Martinez AT.
  Title
A new versatile peroxidase from Pleurotus.
  Journal
Biochem. Soc. Trans. 29 (2001) 116-22.
  Organism
Pleurotus eryngii, Bjerkandera adusta, Phanerochaete chrysosporium
Reference
8  [PMID:12884090]
  Authors
Banci L, Camarero S, Martinez AT, Martinez MJ, Perez-Boada M, Pierattelli R, Ruiz-Duenas FJ.
  Title
NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii.
  Journal
J. Biol. Inorg. Chem. 8 (2003) 751-60.
  Organism
Pleurotus eryngii
  Sequence
[up:O94753]
Reference
9  [PMID:16246366]
  Authors
Perez-Boada M, Ruiz-Duenas FJ, Pogni R, Basosi R, Choinowski T, Martinez MJ, Piontek K, Martinez AT.
  Title
Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways.
  Journal
J. Mol. Biol. 354 (2005) 385-402.
  Organism
Pleurotus eryngii
  Sequence
[up:O94753]
Reference
10 [PMID:10049842]
  Authors
Caramelo L, Martinez MJ, Martinez AT.
  Title
A search for ligninolytic peroxidases in the fungus pleurotus eryngii involving alpha-keto-gamma-thiomethylbutyric acid and lignin model dimers
  Journal
Appl. Environ. Microbiol. 65 (1999) 916-22.
  Organism
Pleurotus eryngii
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
42613-30-9 114995-15-2

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