KEGG   ENZYME: 1.11.1.16Help
Entry
EC 1.11.1.16                Enzyme                                 

Name
versatile peroxidase;
VP;
hybrid peroxidase;
polyvalent peroxidase;
reactive-black-5:hydrogen-peroxide oxidoreductase
Class
Oxidoreductases;
Acting on a peroxide as acceptor;
Peroxidases
BRITE hierarchy
Sysname
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol:hydrogen-peroxide oxidoreductase
Reaction(IUBMB)
(1) 1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol + H2O2 = 4-hydroxy-3-methoxybenzaldehyde + 2-methoxyphenol + glycolaldehyde + H2O [RN:R07612];
(2) 2 manganese(II) + 2 H+ + H2O2 = 2 manganese(III) + 2 H2O [RN:R03532]
Reaction(KEGG)
Substrate
1-(4-hydroxy-3-methoxyphenyl)-2-(2-methoxyphenoxy)propane-1,3-diol;
H2O2 [CPD:C00027];
manganese [CPD:C00034];
H+ [CPD:C00080]
Product
4-hydroxy-3-methoxybenzaldehyde [CPD:C00755];
2-methoxyphenol;
glycolaldehyde [CPD:C00266];
H2O [CPD:C00001];
manganese [CPD:C00034]
Comment
A hemoprotein. This ligninolytic peroxidase combines the substrate-specificity characteristics of the two other ligninolytic peroxidases, EC 1.11.1.13, manganese peroxidase and EC 1.11.1.14, lignin peroxidase. Unlike these two enzymes, it is also able to oxidize phenols, hydroquinones and both low- and high-redox-potential dyes, due to a hybrid molecular architecture that involves multiple binding sites for substrates [2,4].
History
EC 1.11.1.16 created 2006, modified 2016
Reference
1  [PMID:8647081]
  Authors
Martinez MJ, Ruiz-Duenas FJ, Guillen F, Martinez AT.
  Title
Purification and catalytic properties of two manganese peroxidase isoenzymes from Pleurotus eryngii.
  Journal
Eur. J. Biochem. 237 (1996) 424-32.
Reference
2  [PMID:9654123]
  Authors
Heinfling A, Ruiz-Duenas FJ, Martinez MJ, Bergbauer M, Szewzyk U, Martinez AT.
  Title
A study on reducing substrates of manganese-oxidizing peroxidases from Pleurotus eryngii and Bjerkandera adusta.
  Journal
FEBS. Lett. 428 (1998) 141-6.
Reference
3  [PMID:9987124]
  Authors
Ruiz-Duenas FJ, Martinez MJ, Martinez AT.
  Title
Molecular characterization of a novel peroxidase isolated from the ligninolytic fungus Pleurotus eryngii.
  Journal
Mol. Microbiol. 31 (1999) 223-35.
Reference
4  [PMID:10187820]
  Authors
Camarero S, Sarkar S, Ruiz-Duenas FJ, Martinez MJ, Martinez AT.
  Title
Description of a versatile peroxidase involved in the natural degradation of lignin that has both manganese peroxidase and lignin peroxidase substrate interaction sites.
  Journal
J. Biol. Chem. 274 (1999) 10324-30.
  Sequence
Reference
5  [PMID:10508113]
  Authors
Ruiz-Duenas FJ, Martinez MJ, Martinez AT.
  Title
Heterologous expression of Pleurotus eryngii peroxidase confirms its ability to oxidize Mn(2+) and different aromatic substrates.
  Journal
Appl. Environ. Microbiol. 65 (1999) 4705-7.
Reference
6  [PMID:11004397]
  Authors
Camarero S, Ruiz-Duenas FJ, Sarkar S, Martinez MJ, Martinez AT.
  Title
The cloning of a new peroxidase found in lignocellulose cultures of Pleurotus eryngii and sequence comparison with other fungal peroxidases.
  Journal
FEMS. Microbiol. Lett. 191 (2000) 37-43.
  Sequence
Reference
7  [PMID:11356138]
  Authors
Ruiz-Duenas FJ, Camarero S, Perez-Boada M, Martinez MJ, Martinez AT.
  Title
A new versatile peroxidase from Pleurotus.
  Journal
Biochem. Soc. Trans. 29 (2001) 116-22.
Reference
8  [PMID:12884090]
  Authors
Banci L, Camarero S, Martinez AT, Martinez MJ, Perez-Boada M, Pierattelli R, Ruiz-Duenas FJ.
  Title
NMR study of manganese(II) binding by a new versatile peroxidase from the white-rot fungus Pleurotus eryngii.
  Journal
J. Biol. Inorg. Chem. 8 (2003) 751-60.
Reference
9  [PMID:16246366]
  Authors
Perez-Boada M, Ruiz-Duenas FJ, Pogni R, Basosi R, Choinowski T, Martinez MJ, Piontek K, Martinez AT.
  Title
Versatile peroxidase oxidation of high redox potential aromatic compounds: site-directed mutagenesis, spectroscopic and crystallographic investigation of three long-range electron transfer pathways.
  Journal
J. Mol. Biol. 354 (2005) 385-402.
Reference
10 [PMID:10049842]
  Authors
Caramelo L, Martinez MJ, Martinez AT.
  Title
A search for ligninolytic peroxidases in the fungus pleurotus eryngii involving alpha-keto-gamma-thiomethylbutyric acid and lignin model dimers
  Journal
Appl. Environ. Microbiol. 65 (1999) 916-22.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
42613-30-9 114995-15-2

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