KEGG   ENZYME: 1.12.1.4Help
Entry
EC 1.12.1.4                 Enzyme                                 
Name
hydrogenase (NAD+, ferredoxin);
bifurcating [FeFe] hydrogenase
Class
Oxidoreductases;
Acting on hydrogen as donor;
With NAD+ or NADP+ as acceptor
BRITE hierarchy
Sysname
hydrogen:NAD+, ferredoxin oxidoreductase
Reaction(IUBMB)
2 H2 + NAD+ + 2 oxidized ferredoxin = 5 H+ + NADH + 2 reduced ferredoxin [RN:R09508]
Reaction(KEGG)
Substrate
H2 [CPD:C00282];
NAD+ [CPD:C00003];
oxidized ferredoxin [CPD:C00139]
Product
H+ [CPD:C00080];
NADH [CPD:C00004];
reduced ferredoxin [CPD:C00138]
Comment
The enzyme from Thermotoga maritima contains a [FeFe] cluster (H-cluster) and iron-sulfur clusters. It works in the direction evolving hydrogen as a means of eliminating excess reducing equivalents.
Reference
1  [PMID:10482784]
  Authors
Verhagen MF, O'Rourke T, Adams MW
  Title
The hyperthermophilic bacterium, Thermotoga maritima, contains an unusually complex iron-hydrogenase: amino acid sequence analyses versus biochemical characterization.
  Journal
Biochim. Biophys. Acta. 1412 (1999) 212-29.
  Organism
Thermotoga maritima [GN:tma]
Reference
2  [PMID:19411328]
  Authors
Schut GJ, Adams MW
  Title
The iron-hydrogenase of Thermotoga maritima utilizes ferredoxin and NADH synergistically: a new perspective on anaerobic hydrogen production.
  Journal
J. Bacteriol. 191 (2009) 4451-7.
  Organism
Thermotoga maritima [GN:tma]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
DBGET integrated database retrieval system