KEGG   ENZYME: 1.13.11.51Help
Entry
EC 1.13.11.51               Enzyme                                 

Name
9-cis-epoxycarotenoid dioxygenase;
nine-cis-epoxycarotenoid dioxygenase;
NCED;
AtNCED3;
PvNCED1;
VP14
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
BRITE hierarchy
Sysname
9-cis-epoxycarotenoid 11,12-dioxygenase
Reaction(IUBMB)
(1) a 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal [RN:R09682];
(2) 9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al [RN:R06953];
(3) 9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al [RN:R06952]
Reaction(KEGG)
Substrate
9-cis-epoxycarotenoid [CPD:C19811];
O2 [CPD:C00007];
9-cis-violaxanthin [CPD:C13433];
9'-cis-neoxanthin [CPD:C13431]
Product
2-cis,4-trans-xanthoxin [CPD:C13453];
12'-apo-carotenal [CPD:C19812];
(3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al [CPD:C14045];
(3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al
Comment
Requires iron(II). Acts on 9-cis-violaxanthin and 9'-cis-neoxanthin but not on the all-trans isomers [2,3]. In vitro, it will cleave 9-cis-zeaxanthin. Catalyses the first step of abscisic-acid biosynthesis from carotenoids in chloroplasts, in response to water stress. The other enzymes involved in the abscisic-acid biosynthesis pathway are EC 1.1.1.288 (xanthoxin dehydrogenase), EC 1.2.3.14 (abscisic-aldehyde oxidase) and EC 1.14.13.93 [(+)-abscisic acid 8'-hydroxylase].
History
EC 1.13.11.51 created 2005
Pathway
Carotenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K09840  
9-cis-epoxycarotenoid dioxygenase
Genes
ATH: 
AT1G30100(NCED5) AT1G78390(NCED9) AT3G14440(NCED3) AT3G24220(NCED6) AT4G18350(NCED2) AT4G19170(NCED4)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
POPTR_0001s40420g POPTR_0003s17490g(POPTRDRAFT_757941) POPTR_0004s20200g POPTR_0009s15390g(POPTRDRAFT_556918) POPTR_0009s15400g POPTR_0009s15410g POPTR_0009s15430g(POPTRDRAFT_879748) POPTR_0009s15440g(POPTRDRAFT_876388) POPTR_0011s02230g(POPTRDRAFT_805840) POPTR_0011s08240g POPTR_0011s11370g(POPTRDRAFT_568831) POPTR_0019s12320g(POPTRDRAFT_913328)
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
4330451(NCED1) 4333566(NCED3) 4342424 4352125(NCED2) 9270250(NCED5)
DOSA: 
Os02t0704000-01(Os02g0704000) Os03t0645900-00(Os03g0645900) Os04t0131800-00(Os04g0131800) Os07t0154100-01(Os07g0154100) Os12t0435200-01(Os12g0435200) Os12t0617400-01(Os12g0617400)
OBR: 
BDI: 
SBI: 
SORBI_01g013520(SORBIDRAFT_01g013520) SORBI_02g003230(SORBIDRAFT_02g003230) SORBI_02g003240(SORBIDRAFT_02g003240) SORBI_06g014946(SORBIDRAFT_06g014946)
ZMA: 
100281136 100281391(GRMZM2G417954) 100383768 103626043(GRMZM5G838285) 103627610(GRMZM2G150363) 103632443 103634160 103634451(GRMZM2G330848) 103642321 103643757 103648268(GRMZM2G408158) 103652265 732819(vp14)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
SMO: 
PPP: 
VAL: 
EHX: 
 » show all
Taxonomy
Reference
1  [PMID:9188535]
  Authors
Schwartz SH, Tan BC, Gage DA, Zeevaart JA, McCarty DR.
  Title
Specific oxidative cleavage of carotenoids by VP14 of maize.
  Journal
Science. 276 (1997) 1872-4.
  Sequence
[zma:732819]
Reference
2  [PMID:9342392]
  Authors
Tan BC, Schwartz SH, Zeevaart JA, McCarty DR.
  Title
Genetic control of abscisic acid biosynthesis in maize.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 94 (1997) 12235-40.
  Sequence
[zma:732819]
Reference
3  [PMID:10611388]
  Authors
Qin X, Zeevaart JA.
  Title
The 9-cis-epoxycarotenoid cleavage reaction is the key regulatory step of abscisic acid biosynthesis in water-stressed bean.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 96 (1999) 15354-61.
  Sequence
Reference
4  [PMID:10929129]
  Authors
Thompson AJ, Jackson AC, Symonds RC, Mulholland BJ, Dadswell AR, Blake PS, Burbidge A, Taylor IB.
  Title
Ectopic expression of a tomato 9-cis-epoxycarotenoid dioxygenase gene causes over-production of abscisic acid.
  Journal
Plant. J. 23 (2000) 363-74.
Reference
5  [PMID:11532178]
  Authors
Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T, Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K.
  Title
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.
  Journal
Plant. J. 27 (2001) 325-33.
  Sequence
[ath:AT3G14440]
Reference
6
  Authors
Iuchi, S., Kobayashi, M., Taji, T., Naramoto, M., Seki, M., Kato, T., Tabata, S., Kakubari, Y., Yamaguchi-Shinozaki, K. and Shinozaki, K.
  Title
Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.
  Journal
Plant J. 30 (2002) 611.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
199877-10-6

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