KEGG   ENZYME: 1.13.11.65Help
Entry
EC 1.13.11.65               Enzyme                                 

Name
carotenoid isomerooxygenase;
ninaB (gene name)
Class
Oxidoreductases;
Acting on single donors with incorporation of molecular oxygen (oxygenases);
With incorporation of two atoms of oxygen
BRITE hierarchy
Sysname
zeaxanthin:oxygen 15,15'-oxidoreductase (bond-cleaving, cis-isomerizing)
Reaction(IUBMB)
zeaxanthin + O2 = (3R)-11-cis-3-hydroxyretinal + (3R)-all-trans-3-hydroxyretinal [RN:R10192]
Reaction(KEGG)
Substrate
zeaxanthin [CPD:C06098];
O2 [CPD:C00007]
Product
(3R)-11-cis-3-hydroxyretinal [CPD:C18195];
(3R)-all-trans-3-hydroxyretinal [CPD:C18196]
Comment
The enzyme, characterized from the moth Galleria mellonella and the fruit fly Drosophila melanogaster, is involved in the synthesis of retinal from dietary caroteoids in insects. The enzyme accepts different all-trans carotenoids, including beta-carotene, alpha-carotene and lutein, and catalyses the symmetrical cleavage of the carotenoid and the simultaneous isomerization of only one of the products to a cis configuration. When the substrate is hydroxylated only in one side (as in cryptoxanthin), the enzyme preferentially isomerizes the hydroxylated part of the molecule.
History
EC 1.13.11.65 created 2012 as EC 1.14.13.164, transferred 2012 to EC 1.13.11.65
Orthology
K18048  carotenoid isomerooxygenase
Genes
DME: Dmel_CG9347(ninaB)
DPO: Dpse_GA21719
DAN: Dana_GF16875
DER: Dere_GG19789
DPE: Dper_GL23083
DSE: Dsec_GM24124
DSI: Dsimw501_GD18923(Dsim_GD18923)
DWI: Dwil_GK14193
DYA: Dyak_GE26290
DGR: Dgri_GH14266
MDE: 101891299
AAG: 5577639
AME: 410521(ninaB)
BIM: 100748194
BTER: 100650576
SOC: 105207446
AEC: 105152891
ACEP: 105627461
PBAR: 105429637
HST: 105189528
LHU: 105678748
PGC: 109851872
NVI: 100121134
TCA: 655806
DPA: 109546877
NVL: 108568592
BMOR: 101740058(ninaB-1)
PXY: 105388704
API: 100168974
DNX: 107165925
FCD: 110854110
 » show all
Taxonomy
Reference
1  [PMID:19020100]
  Authors
Oberhauser V, Voolstra O, Bangert A, von Lintig J, Vogt K
  Title
NinaB combines carotenoid oxygenase and retinoid isomerase activity in a single polypeptide.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 19000-5.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.13.11.65
IUBMB Enzyme Nomenclature: 1.13.11.65
ExPASy - ENZYME nomenclature database: 1.13.11.65
BRENDA, the Enzyme Database: 1.13.11.65

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