KEGG   ENZYME: 1.14.11.23Help
Entry
EC 1.14.11.23               Enzyme                                 

Name
flavonol synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction(KEGG)
Substrate
dihydroflavonol [CPD:C15570];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
flavonol [CPD:C01495];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from the satsuma Citrus unshiu also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
History
EC 1.14.11.23 created 2004
Pathway
Flavonoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K05278  
flavonol synthase
Genes
ATH: 
AT5G08640(FLS1) AT5G43935(FLS6) AT5G63590(FLS3) AT5G63595(FLS4) AT5G63600(FLS5)
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
EGR: 
GMX: 
PVU: 
VRA: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj1g3v0705350.1(Lj1g3v0705350.1) Lj1g3v0775780.1(Lj1g3v0775780.1) Lj1g3v1380920.1(Lj1g3v1380920.1) Lj5g3v1865700.1(Lj5g3v1865700.1)
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
VVI: 
SLY: 
SPEN: 
SOT: 
SIND: 
BVG: 
NNU: 
OSA: 
4330843(OJ1004_A11.27)
DOSA: 
Os02t0767300-01(Os02g0767300)
OBR: 
BDI: 
SBI: 
SORBI_03g002040(SORBIDRAFT_03g002040) SORBI_04g034240(SORBIDRAFT_04g034240)
ZMA: 
100272992(GRMZM2G152801) 103627785(GRMZM2G069298)
SITA: 
PDA: 
EGU: 
MUS: 
ATR: 
 » show all
Taxonomy
Reference
1  [PMID:12180990]
  Authors
Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U.
  Title
Functional expression and mutational analysis of flavonol synthase from Citrus unshiu.
  Journal
Eur. J. Biochem. 269 (2002) 4134-42.
  Sequence
Reference
2  [PMID:12620339]
  Authors
Lukacin R, Wellmann F, Britsch L, Martens S, Matern U.
  Title
Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase.
  Journal
Phytochemistry. 62 (2003) 287-92.
Reference
3  [PMID:12782296]
  Authors
Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R.
  Title
Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley.
  Journal
FEBS. Lett. 544 (2003) 93-8.
  Sequence
Reference
4  [PMID:14570878]
  Authors
Turnbull JJ, Nakajima J, Welford RW, Yamazaki M, Saito K, Schofield CJ.
  Title
Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase.
  Journal
J. Biol. Chem. 279 (2004) 1206-16.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
146359-76-4

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