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Entry
EC 1.14.11.31               Enzyme                                 

Name
thebaine 6-O-demethylase;
T6ODM
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
thebaine,2-oxoglutarate:oxygen oxidoreductase (6-O-demethylating)
Reaction(IUBMB)
thebaine + 2-oxoglutarate + O2 = neopinone + formaldehyde + succinate + CO2 [RN:R05126]
Reaction(KEGG)
R05126;
(other) R03698
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Substrate
thebaine [CPD:C06173];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
neopinone [CPD:C06172];
formaldehyde [CPD:C00067];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+. Catalyses a step in morphine biosynthesis. The product neopinione spontaneously rearranges to the more stable codeinone. The enzyme also catalyses the 6-O-demethylation of oripavine to morphinone, with lower efficiency.
History
EC 1.14.11.31 created 2010
Pathway
Isoquinoline alkaloid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K14976  
thebaine 6-O-demethylase
Reference
1  [PMID:20228795]
  Authors
Hagel JM, Facchini PJ
  Title
Dioxygenases catalyze the O-demethylation steps of morphine biosynthesis in opium poppy.
  Journal
Nat. Chem. Biol. 6 (2010) 273-5.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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