KEGG   ENZYME: 1.14.11.37Help
Entry
EC 1.14.11.37               Enzyme                                 

Name
kanamycin B dioxygenase;
kanJ (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
kanamycin-B,2-oxoglutarate:oxygen oxidoreductase (deaminating, 2'-hydroxylating)
Reaction(IUBMB)
kanamycin B + 2-oxoglutarate + O2 = 2'-dehydrokanamycin A + succinate + NH3 + CO2 [RN:R10312]
Reaction(KEGG)
Substrate
kanamycin B [CPD:C00825];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
2'-dehydrokanamycin A [CPD:C20509];
succinate [CPD:C00042];
NH3 [CPD:C00014];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. Found in the bacterium Streptomyces kanamyceticus where it is involved in the conversion of the aminoglycoside antibiotic kanamycin B to kanamycin A.
History
EC 1.14.11.37 created 2013, modified 2013
Reference
1  [PMID:22374809]
  Authors
Sucipto H, Kudo F, Eguchi T
  Title
The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the alpha-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK.
  Journal
Angew. Chem. Int. Ed. Engl. 51 (2012) 3428-31.
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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