KEGG   ENZYME: 1.14.11.6Help
Entry
EC 1.14.11.6                Enzyme                                 

Name
thymine dioxygenase;
thymine 7-hydroxylase;
5-hydroxy-methyluracil dioxygenase;
5-hydroxymethyluracil oxygenase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
thymine,2-oxoglutarate:oxygen oxidoreductase (7-hydroxylating)
Reaction(IUBMB)
thymine + 2-oxoglutarate + O2 = 5-hydroxymethyluracil + succinate + CO2 [RN:R01412]
Reaction(KEGG)
R01412;
(other) R08610 R08611
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Substrate
thymine [CPD:C00178];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
5-hydroxymethyluracil [CPD:C03088];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. Also acts on 5-hydroxymethyluracil to oxidize its -CH2OH group first to -CHO and then to -COOH.
History
EC 1.14.11.6 created 1972, modified 1976 (EC 1.14.11.5 created 1972, incorporated 1976)
Reference
1  [PMID:11946494]
  Authors
Bankel L, Holme E, Lindstedt G, Lindstedt S.
  Title
Oxygenases involved in thymine and thymidine metabolism in Neurospora crassa.
  Journal
FEBS. Lett. 21 (1972) 135-138.
Reference
2  [PMID:4274083]
  Authors
Liu CK, Hsu CA, Abbott MT.
  Title
Catalysis of three sequential dioxygenase reactions by thymine 7-hydroxylase.
  Journal
Arch. Biochem. Biophys. 159 (1973) 180-7.
Reference
3  [PMID:6684117]
  Authors
Warn-Cramer BJ, Macrander LA, Abbott MT.
  Title
Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinis.
  Journal
J. Biol. Chem. 258 (1983) 10551-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37256-67-0

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