KEGG   ENZYME: 1.14.11.9Help
Entry
EC 1.14.11.9                Enzyme                                 

Name
flavanone 3-dioxygenase;
naringenin 3-hydroxylase;
flavanone 3-hydroxylase;
flavanone 3beta-hydroxylase;
flavanone synthase I;
(2S)-flavanone 3-hydroxylase;
naringenin,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating);
F3H;
flavanone,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor
BRITE hierarchy
Sysname
(2S)-flavan-4-one,2-oxoglutarate:oxygen oxidoreductase (3-hydroxylating)
Reaction(IUBMB)
a (2S)-flavan-4-one + 2-oxoglutarate + O2 = a (2R,3R)-dihydroflavonol + succinate + CO2 [RN:R07329]
Reaction(KEGG)
Substrate
(2S)-flavan-4-one [CPD:C02099];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
(2R,3R)-dihydroflavonol [CPD:C21417];
succinate [CPD:C00042];
CO2 [CPD:C00011]
Comment
Requires Fe2+ and ascorbate. This plant enzyme catalyses an early step in the flavonoid biosynthesis pathway, leading to the production of flavanols and anthocyanins. Substrates include (2S)-naringenin, (2S)-eriodictyol, (2S)-dihydrotricetin and (2S)-pinocembrin. Some enzymes are bifuctional and also catalyse EC 1.14.11.23, flavonol synthase.
History
EC 1.14.11.9 created 1983, modified 1989, modified 2004, modified 2016
Pathway
Flavonoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K00475  
naringenin 3-dioxygenase
Genes
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
BNA: 
BOE: 
THJ: 
CIT: 
CIC: 
TCC: 
GRA: 
GHI: 
EGR: 
GMX: 
PVU: 
VRA: 
VAR: 
MTR: 
CAM: 
ADU: 
AIP: 
LJA: 
Lj3g3v1487200.1(Lj3g3v1487200.1) Lj4g3v1037630.1(Lj4g3v1037630.1)
LANG: 
FVE: 
PPER: 
PMUM: 
MDM: 
103400025(F3H) 103436139(MdF3H)
PXB: 
ZJU: 
CSV: 
CMO: 
RCU: 
JCU: 
POP: 
VVI: 
SLY: 
SPEN: 
SOT: 
CANN: 
NTA: 
INI: 
SIND: 
BVG: 
NNU: 
OSA: 
DOSA: 
Os04t0662600-00(Os04g0662600)
OBR: 
BDI: 
ATS: 
SBI: 
ZMA: 
542712(fht1)
SITA: 
PDA: 
EGU: 
MUS: 
DCT: 
ATR: 
 » show all
Taxonomy
Reference
1
  Authors
Forkmann, G., Heller, W. and Grisebach, H.
  Title
Anthocyanin biosynthesis in flowers of Matthiola incana flavanone 3- and flavonoid 3'-hydroxylases.
  Journal
Z. Naturforsch. C: Biosci. 35 (1980) 691-695.
Reference
2  [PMID:8541503]
  Authors
Charrier B, Coronado C, Kondorosi A, Ratet P
  Title
Molecular characterization and expression of alfalfa (Medicago sativa L.) flavanone-3-hydroxylase and dihydroflavonol-4-reductase encoding genes.
  Journal
Plant. Mol. Biol. 29 (1995) 773-86.
Reference
3  [PMID:8685272]
  Authors
Pelletier MK, Shirley BW
  Title
Analysis of flavanone 3-hydroxylase in Arabidopsis seedlings. Coordinate regulation with chalcone synthase and chalcone isomerase.
  Journal
Plant. Physiol. 111 (1996) 339-45.
Reference
4  [PMID:15013767]
  Authors
Wellmann F, Matern U, Lukacin R.
  Title
Significance of C-terminal sequence elements for Petunia flavanone 3beta-hydroxylase activity.
  Journal
FEBS. Lett. 561 (2004) 149-54.
Reference
5  [PMID:16147863]
  Authors
Jin Z, Grotewold E, Qu W, Fu G, Zhao D
  Title
Cloning and characterization of a flavanone 3-hydroxylase gene from Saussurea medusa.
  Journal
DNA. Seq. 16 (2005) 121-9.
Reference
6  [PMID:16779664]
  Authors
Shen G, Pang Y, Wu W, Deng Z, Zhao L, Cao Y, Sun X, Tang K
  Title
Cloning and characterization of a flavanone 3-hydroxylase gene from Ginkgo biloba.
  Journal
Biosci. Rep. 26 (2006) 19-29.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
75991-43-4

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